FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7503707
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG86526111.531113888108905No Hit
CAACGGAATCCCAAAAGCAGCTG6025918.030577419933907No Hit
CCCCCACTGCTAAATTTGACTG2732723.6418266331561187No Hit
TTCAAGTAATCCAGGATAGGCT2543373.38948469070021No Hit
TAGCAGCACGTAAATATTGGCG2509143.343867237886554No Hit
TAGCTTATCAGACTGATGTTGAC2384323.1775227897357934No Hit
AAGCTGCCAGTTGAAGAACTGT2237652.982059400773511No Hit
TAGCTTATCAGACTGATGTTGACT2026152.7001987151150755No Hit
AACATTCAACGCTGTCGGTGAGT1459001.944372294920364No Hit
TAGCTTATCAGACTGATGTTGA1411141.8805904868087202No Hit
AACATTCAACGCTGTCGGTGAG1272111.6953087320706952No Hit
TGTAAACATCCTTGACTGGAAGCT1093771.4576395373646653No Hit
TATTGCACTTGTCCCGGCCTGTAA1006221.3409638729230766No Hit
TGAGGTAGTAGGTTGTATAGTT854621.1389303980019476No Hit
TATTGCACTTGTCCCGGCCTGTAT820721.093752727818397No Hit
TGAGGTAGTAGATTGTATAGTT647690.8631600354331532No Hit
CATAAAGTAGAAAGCACTACT600080.79971139598068No Hit
TTCAAGTAATTCAGGATAGGTT513470.6842884456975732No Hit
AGCAGCATTGTACAGGGCTATGA477300.6360856040887524No Hit
TGTAAACATCCCCGACTGGAAGCT450220.5999967749273792No Hit
GCCTGTCTGAGCGTCGCT426850.5688521686680996No Hit
TAGCTTATCAGACTGATGTTGACA403690.5379874240825234No Hit
TATTGCACTTGTCCCGGCCTGTA389600.5192100384516612No Hit
TATTGCACTTGTCCCGGCCTGT349820.4661962414044152No Hit
ACTGGACTTGGAGTCAGAAGGC343450.4577071039687451No Hit
TTCTCACTACTGCACTTGACTAG341290.45482852675351No Hit
CAACGGAATCCCAAAAGCAGCTGT338560.4511903249953656No Hit
CCCCCACTGCTAAATTTGACTGGCTT338410.45099042379986315No Hit
CAAAGAATTCTCCTTTTGGGCT324030.4318265625243629No Hit
CTTCTCACTACTGCACTTGACTAG282780.37685373376119297No Hit
TGTAAACATCCTACACTCTCAGCT266600.3552910581396635No Hit
CAACGGAATCCCAAAAGCAGCT251710.335447532799455No Hit
CCCATAAAGTAGAAAGCACTAC245840.32762473268212633No Hit
ACCCCCCACTGCTAAATTTGACTG236940.31576392841564843No Hit
TGAGGTAGTAGTTTGTACAGTT223300.29758624637129355No Hit
TAGCAGCACGTAAATATTGGC221490.2951741052788975No Hit
CAAAGAATTCTCCTTTTGGGCTT181750.24221361521711868No Hit
CATTGCACTTGTCTCGGTCTGA181480.24185379306521432No Hit
AACCGGGCGGAAACACCA173530.2312590297035852No Hit
CCCCCCACTGCTAAATTTGACTGGCT167170.22278321901428189No Hit
TCCTGTCTGAGCGTCGCT166700.22215686193504092No Hit
TTCACAGTGGCTAAGTTCCG160840.21434738856407906No Hit
TGTCAGTTTGTCAAATACCCCA160800.21429408157861174No Hit
CTCCCACTGCTTCACTTGACTAG154510.20591155811387624No Hit
TGTCAGTTTGTCAAATACCCCAA153540.20461886371629384No Hit
CCCATAAAGTAGAAAGCACT152750.20356605075331435No Hit
TGAGGTAGTAGTTTGTGCTGTT143920.19179853371140426No Hit
CCCCCCACTGCTAAATTTGACTGG141960.18918649142350574No Hit
TTCAAGTAATCCAGGATAGGCTT141460.1885201541051643No Hit
TTATAAAGCAATGAGACTGATT136050.18131038432070973No Hit
AACGGAATCCCAAAAGCAGCTG132820.17700584524422397No Hit
TTAATGCTAATCGTGATAGGGGTT129370.1724081177476679No Hit
AACATTCAACGCTGTCGGTGAGTT128350.17104878961825135No Hit
TCGTTTCCCGGCCAATGCACCA123500.16458531763033926No Hit
TGTAAACATCCCCGACTGGAAGC122690.16350585117462607No Hit
ATCACATTGCCAGGGATTTCC121310.16166676017600368No Hit
CCCCCCACTGCTAAATTTGACTGTA117480.15656261631750815No Hit
GGGAATACCGGGTGCTGTAGGCTTT117420.15648265583930718No Hit
GGAATACCGGGTGCTGTAGGCTTT116860.15573635804276473No Hit
ATCACATTGCCAGGGATTTCCA112800.15032569901783213No Hit
CAACACCAGTCGATGGGCTGT111820.14901967787388287No Hit
CACTAGATTGTGAGCTCCTGGAA110850.14772698347630045No Hit
CACCCGGCTGTGTGCACATGTGC109030.14530151563753754No Hit
TATTGCACTTGTCCCGGCCTGTAG108550.14466183181192976No Hit
TTCAAGTAATCCAGGATAGGC107060.14267614660327224No Hit
TTCACAGTGGCTAAGTTCTGC104840.13971760890983617No Hit
AGCTCGGTCTGAGGCCCCTCAGT102730.13690566542643523No Hit
TTCACAGTGGCTAAGTTCCGC100600.13406706845030064No Hit
TAGCAGCACGTAAATATTGGCGT99200.13220132395894457No Hit
TATTGCACTTGTCCCGGCCTGTAAA98600.13140171917693483No Hit
ATGCACCTGGGCAAGGATTCTGA98170.13082866908316115No Hit
CAGTGCAATAGTATTGTCAAAGC98100.13073538185859337No Hit
TATTGCACTTGTCCCGGCCTGTAAT93640.12479165297898757No Hit
ACTGGACTTGGAGTCAGAAGGCT93470.12456509829075149No Hit
TGTAAACATCCTACACTCAGCT93380.12444515757345004No Hit
AGCAGCATTGTACAGGGCTATGT91310.1216865210755164No Hit
TGTAAACATCCTTGACTGGAAGC89050.11867467639661304No Hit
AATGCACCCGGGCAAGGATTCT88960.11855473567931157No Hit
CACTAGATTGTGAGCTCCTGGA88740.11826154725924133No Hit
TTCACAGTGGCTAAGTTCTG87650.11680893190525697No Hit
CTCCCACTGCTTCACTTGACTAGCCT87630.11678227841252331No Hit
TATTGCACTTGTCCCGGCCTGTT87430.11651574348518672No Hit
TAGCAGCACATCATGGTTTACA87010.1159560201377799No Hit
CCCCCCACTGCTAAATTTGACTGT83270.11097181699658581No Hit
AGCTCTAGAATTACTCTGAGACCT83000.11061199484468144No Hit
ACTGGACTTGGAGTCAGAAGGCA82850.110412093649179No Hit
CTACGCCTGTCTGAGCGTCGCT82600.11007892499000826No Hit
CAACGGAATCCCAAAAGCAGCTGA82140.10946589465713413No Hit
GGGAATACCGGGTGCTGTAGGCTT80520.10730696174570782No Hit
CAAAGAATTCTCCTTTTGGGC78020.10397527515400055No Hit
AGAATTACTCTGAGACCT77640.10346885879206104No Hit
TGAGGTAGTAAGTTGTATTGTT77270.10297576917648837No Hit
TTTAAACATCCTTGACTGGAAGCT75060.10003055822941913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)