FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11237781
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG186248316.573405372466326No Hit
CAACGGAATCCCAAAAGCAGCTG128627911.446023018245329No Hit
CCCCCACTGCTAAATTTGACTG5821915.180657996449655No Hit
TTCAAGTAATCCAGGATAGGCT3732523.3214030421130296No Hit
AACATTCAACGCTGTCGGTGAGT2199851.9575483807701892No Hit
TAGCAGCACGTAAATATTGGCG1603201.426616162034124No Hit
AAGCTGCCAGTTGAAGAACTGT1489651.3255730824439451No Hit
AACATTCAACGCTGTCGGTGAG1280041.1390504940432635No Hit
TCGTACGACTCTTAGCGG1141281.015574160058823No Hit
CATAAAGTAGAAAGCACTACT1077310.9586501107291555No Hit
CCCATAAAGTAGAAAGCACTAC865440.7701164491459658No Hit
CTCCCACTGCTTCACTTGACTAG793650.7062337306626637No Hit
CAACGGAATCCCAAAAGCAGCTGT739630.6581637424683752No Hit
TAGCTTATCAGACTGATGTTGA649880.5782992211718666No Hit
CGCCTGTCTGAGCGTCGCT623260.5546112706770135No Hit
CAACACCAGTCGATGGGCTGT618570.5504378488956139No Hit
CCCATAAAGTAGAAAGCACT595620.5300156676838603No Hit
ACTGGACTTGGAGTCAGAAGGC567650.5051264124118454No Hit
CATTGCACTTGTCTCGGTCTGA562090.500178816440719No Hit
AGCAGCATTGTACAGGGCTATGA559310.4977050184551558No Hit
TAGCTTATCAGACTGATGTTGAC546370.4861902897022108No Hit
CAACGGAATCCCAAAAGCAGCT516200.459343352571117No Hit
TTCAAGTAATTCAGGATAGGTT459860.4092088998708909No Hit
ATCCCGGACGAGCCCCCA449930.4003726358433217No Hit
ACCGGGTGCTGTAGGCTTA442040.3933516768123529No Hit
CCCCCCACTGCTAAATTTGACTGG406440.3616728249108966No Hit
CCCCCACTGCTAAATTTGACTGGCTT399120.35515908345250724No Hit
CTACGCCTGTCTGAGCGTCGCT398910.35497221382050426No Hit
GCCTGTCTGAGCGTCGCT375490.3341318005752203No Hit
GACCCGCGGGCGCTCTCCAGTCCT373380.3322542057012857No Hit
TATTGCACTTGTCCCGGCCTGTAA364600.32444127537278045No Hit
TATTGCACTTGTCCCGGCCTGT359840.32020556371404635No Hit
TTCTCACTACTGCACTTGACTAG336210.2991782808367595No Hit
CCCCCCACTGCTAAATTTGACTGGCT335010.2981104543681711No Hit
TGAGGTAGTAGGTTGTATAGTT334790.2979146861822632No Hit
TAGCTTATCAGACTGATGTTGACT334460.2976210339034014No Hit
CACTAGATTGTGAGCTCCTGGA329190.29293149599551727No Hit
CTTCTCACTACTGCACTTGACTAG317540.28256468069630475No Hit
AGCTCGGTCTGAGGCCCCTCAGT300940.26779308121416495No Hit
TATTGCACTTGTCCCGGCCTGTAT294740.26227597779312484No Hit
CACTAGATTGTGAGCTCCTGGAA285140.2537333660444175No Hit
ACCGGGTGCTGTAGGCTTT280030.2491862049990118No Hit
ACCGGGTGCTGTAGGCTT272430.24242330403128515No Hit
TACGCCTGTCTGAGCGTCGCT266350.2370129832571039No Hit
CAAAGAATTCTCCTTTTGGGCT261310.23252811208903254No Hit
TGTAAACATCCTACACTCTCAGCT258350.22989414013318107No Hit
TGAGGTAGTAGATTGTATAGTT243440.21662639626097002No Hit
AACCGGGCGGAAACACCA234700.2088490601480844No Hit
ACCCCCCACTGCTAAATTTGACTG226020.20112511535862818No Hit
AACGGAATCCCAAAAGCAGCTG222670.1981440998004855No Hit
CACCCGGCTGTGTGCACATGTGC216950.19305412696688076No Hit
ATCCCACCGCTGCCACCA211120.18786627004032203No Hit
TTCACAGTGGCTAAGTTCCG207310.1844759210025538No Hit
TTCAAGTAATCCAGGATAGGCTT206890.18410218173854784No Hit
CAAAGAATTCTCCTTTTGGGCTT205560.1829186740691957No Hit
CCCATAAAGTAGAAAGCACTA194480.17305907634256265No Hit
ACGCCTGTCTGAGCGTCGCT193980.17261414864731747No Hit
TATTGCACTTGTCCCGGCCTGTA191400.17031832173985237No Hit
CCCCCCACTGCTAAATTTGACTGT182940.16279014513630405No Hit
AACATTCAACGCTGTCGGTGAGTT178200.15857223058537978No Hit
ACCACAGGGTAGAACCACGGAC172460.1534644606439652No Hit
ACTGGACTTGGAGTCAGAAGGCT169380.15072370604125493No Hit
TTCACAGTGGCTAAGTTCCGC159620.14203871743006916No Hit
TCGTTTCCCGGCCAATGCACCA155510.13838141177515384No Hit
TCCTGTCTGAGCGTCGCT152260.1354893817560602No Hit
TCGTTTCCCGGGCGGCGCACCA151310.13464401913509438No Hit
CCCCCCACTGCTAAATTTGACTGTA148540.13217911970343613No Hit
TCCCACTGCTTCACTTGACTAG147640.1313782498519948No Hit
TGTAAACATCCTTGACTGGAAGCT141680.12607471172467233No Hit
CGCGACCTCAGATCAGACGTGGCG140840.12532723319666045No Hit
TTCAAGTAATCCAGGATAGGC137320.12219494222213442No Hit
ACTGGACTTGGAGTCAGAAGG136220.12121610129259505No Hit
ACTGGACTTGGAGTCAGAAGGCA131740.11722954914319829No Hit
CAACGGAATCCCAAAAGCAGCTGA131540.11705157806510022No Hit
CCCCCACTGCTAAATTTGACTGGCT130950.11652656338471092No Hit
CGCGACCTCAGATCAGACGTGGC130680.11628630242927851No Hit
CTCCCACTGCTTCACTTGACTA129010.11480024392715965No Hit
CTCCCACTGCTTCACTTGACTAGCCT124240.11055563371452069No Hit
CCCCCACTGCTAAATTTGACTGG122670.10915856075145085No Hit
CGCGACCTCAGATCAGACGT120180.10694282082912988No Hit
TGTAAACATCCTACACTCAGCT117340.10441563152013729No Hit
AGCAGCATTGTACAGGGCTATGT116670.10381942840850876No Hit
CAAAGTGCTGTTCGTGCAGGT116350.10353467468355185No Hit
GACCCGCGGGCGCTCTCCAGTCCTT113060.10060705044883861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)