FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9991068
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7530037.536761835671622No Hit
CAACGGAATCCCAAAAGCAGCTG5999006.004363097118346No Hit
AAGCTGCCAGTTGAAGAACTGT3968633.972177949344354No Hit
TAGCAGCACGTAAATATTGGCG3438273.441343808289564No Hit
AACATTCAACGCTGTCGGTGAGT3152943.1557587236920015No Hit
TTCAAGTAATCCAGGATAGGCT3112693.1154727402515925No Hit
CCCCCACTGCTAAATTTGACTG2251552.2535628823665297No Hit
TAGCTTATCAGACTGATGTTGAC1789551.7911498550505314No Hit
AACATTCAACGCTGTCGGTGAG1644031.6454997603859767No Hit
TAGCTTATCAGACTGATGTTGA1413251.4145134434076518No Hit
GCCTGTCTGAGCGTCGCT1409731.4109902965328631No Hit
TAGCTTATCAGACTGATGTTGACT1293461.2946163513250035No Hit
TATTGCACTTGTCCCGGCCTGTAA1071901.0728582770130282No Hit
CGCCTGTCTGAGCGTCGCT1051371.0523099232234232No Hit
TGAGGTAGTAGGTTGTATAGTT1047071.0480060790297894No Hit
TATTGCACTTGTCCCGGCCTGT995950.9968403778254737No Hit
CATAAAGTAGAAAGCACTACT986800.9876821977390204No Hit
TATTGCACTTGTCCCGGCCTGTAT926080.9269079141489177No Hit
TGTAAACATCCTTGACTGGAAGCT887910.8887037902254294No Hit
AGCAGCATTGTACAGGGCTATGA677030.6776352638176419No Hit
TGAGGTAGTAGATTGTATAGTT674810.6754132791409286No Hit
TTCAAGTAATTCAGGATAGGTT618730.6192831437039563No Hit
TATTGCACTTGTCCCGGCCTGTA573070.573582323731557No Hit
TCGTACGACTCTTAGCGG522200.5226668460268712No Hit
TTCTCACTACTGCACTTGACTAG486310.48674476042000714No Hit
ACTGGACTTGGAGTCAGAAGGC461930.46234296473610226No Hit
CCCATAAAGTAGAAAGCACTAC436980.4373706594730413No Hit
TGTAAACATCCCCGACTGGAAGCT430160.43054456240313843No Hit
CTACGCCTGTCTGAGCGTCGCT406340.4067032673584045No Hit
TACGCCTGTCTGAGCGTCGCT379910.3802496389775347No Hit
AACCGGGCGGAAACACCA366030.36635723027808437No Hit
CAACGGAATCCCAAAAGCAGCTGT350630.35094346270088445No Hit
CATTGCACTTGTCTCGGTCTGA342690.3429963643526398No Hit
TGTAAACATCCTACACTCTCAGCT323130.32341887774159883No Hit
ACGCCTGTCTGAGCGTCGCT299650.2999178866563615No Hit
CTTCTCACTACTGCACTTGACTAG295160.295423872603009No Hit
TGTCAGTTTGTCAAATACCCCAA292760.29302172700656226No Hit
CCCATAAAGTAGAAAGCACT289430.2896887499914924No Hit
CTCCCACTGCTTCACTTGACTAG287220.28747677425476437No Hit
GCGTTCGCGCTTTCCCCTG282040.28229214334243347No Hit
TGAGGTAGTAGTTTGTACAGTT276320.2765670296709021No Hit
AACATTCAACGCTGTCGGTGAGTT272800.27304388279611347No Hit
TTAATGCTAATCGTGATAGGGGTT259910.26014235915519746No Hit
CAACACCAGTCGATGGGCTGT254630.2548576388430146No Hit
GACCCGCGGGCGCTCTCCAGTCCT250180.2504036605496029No Hit
ACCGGGTGCTGTAGGCTT243740.24395790319913746No Hit
TGAGGTAGTAGTTTGTGCTGTT226960.22716290190398064No Hit
ACCGGGTGCTGTAGGCTTT222670.22286906665033207No Hit
CCCCCCACTGCTAAATTTGACTGG216330.21652339869971857No Hit
TAGCTTATCAGACTGATGTTGACA215340.2155325136411843No Hit
TTCACAGTGGCTAAGTTCCG204210.20439256343766252No Hit
ACCGGGTGCTGTAGGCTTA202750.2029312581998241No Hit
ATCCCACCGCTGCCACCA201970.20215056088097888No Hit
ATCCCGGACGAGCCCCCA196930.1971060551284407No Hit
TAGCAGCACGTAAATATTGGC194840.19501418667153503No Hit
TAGCAGCACGTAAATATTGGCGT193210.19338272945394827No Hit
TGTCAGTTTGTCAAATACCCCA189590.18975949317930776No Hit
TGTAAACATCCTACACTCAGCT180180.18034108065323948No Hit
TTATAAAGCAATGAGACTGATT175850.17600720963965014No Hit
GGAATACCGGGTGCTGTAGGCTTT173190.17334483160358832No Hit
CAAAGAATTCTCCTTTTGGGCT172400.17255412534475795No Hit
CCCCCACTGCTAAATTTGACTGGCTT169590.1697416132089182No Hit
TTCAAGTAATCCAGGATAGGCTT162850.16299558765889693No Hit
GGGCTACGCCTGTCTGAGCGTCGCT162080.16222489928003692No Hit
CAACGGAATCCCAAAAGCAGCT158450.15859165406541123No Hit
TATTGCACTTGTCCCGGCCTGTT155810.1559492939093198No Hit
AACGGAATCCCAAAAGCAGCTG146970.14710139096240762No Hit
CAAAGAATTCTCCTTTTGGGCTT146560.14669102442301463No Hit
GGAATACCGGGTGCTGTAGGCTT143720.14384848546721932No Hit
TGTAAACATCCCCGACTGGAAGC142640.14276751994881828No Hit
TTCACAGTGGCTAAGTTCCGC142550.14267743948895154No Hit
TTCAAGTAATCCAGGATAGGC141720.14184669747018036No Hit
AGCTCGGTCTGAGGCCCCTCAGT141310.1414363309307874No Hit
TGAGGTAGTAGGTTGTGTGGTT136990.13711246885718323No Hit
AGCAGCATTGTACAGGGCTATGT136460.13658199503796792No Hit
CCCCCCACTGCTAAATTTGACTGGCT136040.1361616195585897No Hit
TCGTTTCCCGGCCAATGCACCA136000.13612158379864894No Hit
CCTCGACACAAGGGTTTGT129850.12996608570775417No Hit
AGCAGCATTGTACAGGGCTATG128400.1285147894099009No Hit
ACCACAGGGTAGAACCACGGAC128050.12816447651041912No Hit
ACCCCCCACTGCTAAATTTGACTG127510.12762399375121858No Hit
TTCACAGTGGCTAAGTTCTGC127200.12731371661167754No Hit
ACTGGACTTGGAGTCAGAAGGCA126390.12650299247287677No Hit
TTCACCACCTTCTCCACCCAGC121860.12196894265958354No Hit
CACTAGATTGTGAGCTCCTGGA121240.12134838838050147No Hit
CAAAGTGCTGTTCGTGCAGGT121200.12130835262056068No Hit
TACCTGGTTGATCCTGCC120390.12049762848175992No Hit
TGAGAACTGAATTCCATGGGTT114190.11429208569093915No Hit
TATTGCACTTGTCCCGGCCTGTAG113550.11365151353188668No Hit
ACTGGACTTGGAGTCAGAAGG111800.11189994903447759No Hit
TGATGTAGTAGGTTGTATAGTT110460.1105587510764615No Hit
TGAGGTAGTAGGTTGTATAGT109880.1099782325573202No Hit
CTCGACACAAGGGTTTGT109200.10929762463832696No Hit
CAAAGTGCTGTTCGTGCAGGTAG108820.10891728491888954No Hit
CGCGACCTCAGATCAGACGTGGCG108080.10817662335998514No Hit
CCAAGTCCTTCTGATCGAGGCCC106180.10627492476279812No Hit
TCGTTTCCCGGGCGGCGCACCA101730.10182094646938646No Hit
CGCGACCTCAGATCAGACGTGG101510.10160074978971217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)