FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264505
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6329757.658958401017363No Hit
AACATTCAACGCTGTCGGTGAGT4285895.185900425978325No Hit
AAGCTGCCAGTTGAAGAACTGT2537733.0706376243949274No Hit
CAACGGAATCCCAAAAGCAGCTG2002992.423605527493782No Hit
CCCCCACTGCTAAATTTGACTG1891512.288715416107801No Hit
TTCAAGTAATCCAGGATAGGCT1796332.1735482040364182No Hit
CGCCTGTCTGAGCGTCGCT1652641.999684191612202No Hit
TAGCAGCACGTAAATATTGGCG1641811.986579958509312No Hit
TAGCTTATCAGACTGATGTTGAC1515711.8339997374313404No Hit
AACATTCAACGCTGTCGGTGAG1365811.6526216633664086No Hit
TCCACCACGTTCCCGTGG1338911.6200728295282054No Hit
TATTGCACTTGTCCCGGCCTGT1177271.4244894279814702No Hit
TATTGCACTTGTCCCGGCCTGTAA1089451.3182277704472318No Hit
TAGCTTATCAGACTGATGTTGACT1041481.2601843667588077No Hit
TATTGCACTTGTCCCGGCCTGTAT992711.2011729680119982No Hit
GCCTGTCTGAGCGTCGCT925271.1195709845901236No Hit
TAGCTTATCAGACTGATGTTGA903591.093338318507884No Hit
TGAGGTAGTAGGTTGTATAGTT860961.0417562818341812No Hit
TGAGGTAGTAGATTGTATAGTT705750.8539531405692173No Hit
ACCGGGTGCTGTAGGCTT693650.8393122153111409No Hit
ACCGGGTGCTGTAGGCTTT657350.7953894395369112No Hit
TATTGCACTTGTCCCGGCCTGTA606350.733679754564853No Hit
ACTGGACTTGGAGTCAGAAGGC557300.6743295575476087No Hit
ACCGGGTGCTGTAGGCTTA530490.6418896231534738No Hit
GCGTTCGCGCTTTCCCCTG498390.6030488214357665No Hit
TACGCCTGTCTGAGCGTCGCT474360.5739726698695203No Hit
CATAAAGTAGAAAGCACTACT467280.5654059136028111No Hit
AGCAGCATTGTACAGGGCTATGA459210.5556412634513501No Hit
ATCCCGGACGAGCCCCCA432750.5236248268952587No Hit
TCCTGTCTGAGCGTCGCT423900.5129163815618721No Hit
CTACGCCTGTCTGAGCGTCGCT391450.47365208200612136No Hit
AACATTCAACGCTGTCGGTGAGTT369930.4476130149355588No Hit
TGTAAACATCCTTGACTGGAAGCT360310.4359728743584764No Hit
AACCGGGCGGAAACACCA351720.425579027419065No Hit
TGTAAACATCCTACACTCTCAGCT310230.3753763837035612No Hit
ACGCCTGTCTGAGCGTCGCT305730.3699314115001443No Hit
TGTAAACATCCCCGACTGGAAGCT275760.3336678966253877No Hit
CTCCCACTGCTTCACTTGACTAG274010.33155040743517006No Hit
TTCTCACTACTGCACTTGACTAG264180.31965616815526154No Hit
TTAATGCTAATCGTGATAGGGGTT261890.31688528230063384No Hit
TCGTTCGCGCTTTCCCCTG247580.29957027069376807No Hit
TGAGGTAGTAGTTTGTGCTGTT243230.2943067975637984No Hit
TTCAAGTAATTCAGGATAGGTT228050.27593909133093875No Hit
TGAGGTAGTAGTTTGTACAGTT226920.2745717983109696No Hit
CTTCTCACTACTGCACTTGACTAG217430.26308895693087486No Hit
CCCATAAAGTAGAAAGCACT213870.2587813789210606No Hit
AACATTCAACGCTGTCGGTGA206430.249779024878078No Hit
AGCTCGGTCTGAGGCCCCTCAGT206140.24942812666941336No Hit
TAGCAGCACATCATGGTTTACA205870.24910142833720833No Hit
CATTGCACTTGTCTCGGTCTGA203570.24631844254435084No Hit
TTATAAAGCAATGAGACTGATT196800.23812678436276585No Hit
GGAATACCGGGTGCTGTAGGCTTT194240.2350292001759331No Hit
TGTAAACATCCTACACTCAGCT182660.22101747170580693No Hit
CAACACCAGTCGATGGGCTGT181840.2200252767709621No Hit
TTCACAGTGGCTAAGTTCCG178810.21635899548732804No Hit
TCGTACGACTCTTAGCGG170310.206074047991985No Hit
CCCATAAAGTAGAAAGCACTAC170100.20581994928915887No Hit
CACTAGATTGTGAGCTCCTGGA166810.20183906961154965No Hit
TATTGCACTTGTCCCGGCCTGTT161240.1950994040175425No Hit
CCAAGTCCTTCTGATCGAGGCCC160240.19388941019456096No Hit
TTCAAGTAATCCAGGATAGGCTT155060.1876216421915166No Hit
CCTCGACACAAGGGTTTGT152860.18495965578095724No Hit
GACCCGCGGGCGCTCTCCAGTCCT150640.18227346949393824No Hit
TCAGGCTCAGTCCCCTCCCGAT149600.18101507591803742No Hit
ACTGGACTTGGAGTCAGAAGG148430.17959938314514903No Hit
GGAATACCGGGTGCTGTAGGCTT144020.17426331038580048No Hit
TATTGCACTTGTCCCGGCCTGTAG140760.17031873052288068No Hit
CAAGTCCTTCTGATCGAGGCCC130250.15760169544334476No Hit
CTGCGTTCGCGCTTTCCCCTG130130.15745649618458699No Hit
AACATTCAACGCTGTCGGTGAGA128850.1559077040911706No Hit
ACCACAGGGTAGAACCACGGAC125800.15221722293107695No Hit
ACTGGACTTGGAGTCAGAAGGCA123800.14979723528511388No Hit
TAGCTTATCAGACTGATGTTGACA120070.14528395832539273No Hit
GAATACCGGGTGCTGTAGGCTTT114380.13839909347262783No Hit
AACATTCATTGCTGTCGGTGGGT112550.13618480477657163No Hit
CAACGGAATCCCAAAAGCAGCTGT112200.1357613069385281No Hit
TGAGGTAGTAGGTTGTGTGGTT111120.134454513609708No Hit
CCCCCCACTGCTAAATTTGACTGG108010.13069143282023546No Hit
CAAAGAATTCTCCTTTTGGGCTT107370.12991703677352726No Hit
ATACCGGGTGCTGTAGGCTTT106200.12850134400063887No Hit
CTCGACACAAGGGTTTGT102610.12415746617613517No Hit
CAAAGAATTCTCCTTTTGGGCT102140.12358876907933385No Hit
CACTAGATTGTGAGCTCCTGGAA101330.12260867408271882No Hit
ATACCGGGTGCTGTAGGCTT100370.12144708001265653No Hit
AGCAGCATTGTACAGGGCTATG99340.12020078637498556No Hit
ATCCCACCGCTGCCACCA98870.11963208927818425No Hit
TCTCCCAACCCTTGTACCAGTG97560.11804699737007843No Hit
TGAGGTAGTAGGTTGTATAGT94110.11387251868079212No Hit
TGAATACCGGGTGCTGTAGGCTTT94100.11386041874256234No Hit
AATGACACGATCACTCCCGTTG91590.11082333424687868No Hit
TCCCACTGCTTCACTTGACTAG90640.10967384011504622No Hit
TTCACAGTGGCTAAGTTCTG90210.10915354277116415No Hit
CCTGTCTGAGCGTCGCTT89040.10773784999827576No Hit
TAGCAGCACATAATGGTTTGT88900.10756845086305836No Hit
TGAGGTAGTAGTTTGTGCTGTTA88280.10681825469280978No Hit
TGAGGTAGTAGATTGTATAGT86960.10522106284647417No Hit
CAAAGTGCTGTTCGTGCAGGT86320.104446666799766No Hit
CCCCCCACTGCTAAATTTGACTGGCT85630.10361177106190872No Hit
ACTGGACTTGGAGTCAGAAGGCT84950.1027889752622813No Hit
TGTAAACATCCCCGACTGGAAGC83020.10045368718392693No Hit
TTCAAGTAATCCAGGATAGGC82690.10005438922234301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)