FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7406550
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG81028810.940154322862872No Hit
CCCCCCACTGCTAAATTTGACTG6975329.4177721071214No Hit
CCCCCACTGCTAAATTTGACTG3364394.542452288852434No Hit
TCGTACGACTCTTAGCGG2958103.9938972936117354No Hit
TTCAAGTAATCCAGGATAGGCT2300393.1058860063052296No Hit
AACATTCAACGCTGTCGGTGAG1786242.4117031546401497No Hit
TAGCAGCACGTAAATATTGGCG1571282.1214735605646355No Hit
TGAGGTAGTAGATTGTATAGTT1567312.116113440130695No Hit
AAGCTGCCAGTTGAAGAACTGT1272111.7175473061006812No Hit
TAGCTTATCAGACTGATGTTGAC962981.3001734950820556No Hit
TAGCTTATCAGACTGATGTTGA952851.2864964119596844No Hit
TGAGGTAGTAGGTTGTATAGTT868621.1727727484456325No Hit
TGAGAACTGAATTCCATGGGTT842531.137547171085053No Hit
TATTGCACTTGTCCCGGCCTGT838291.1318225084553537No Hit
GCCTGTCTGAGCGTCGCT793991.0720105852252397No Hit
AACATTCAACGCTGTCGGTGAGT588300.7942969398707901No Hit
TAGCTTATCAGACTGATGTTGACT570900.7708042205885332No Hit
CATAAAGTAGAAAGCACTACT553550.7473790091203056No Hit
TGAGGTAGTAGTTTGTACAGTT537040.7250879289277734No Hit
TATTGCACTTGTCCCGGCCTGTAA530960.7168789787417894No Hit
ACCGGGTGCTGTAGGCTT520630.7029318643633No Hit
TATTGCACTTGTCCCGGCCTGTAT427880.577704869338626No Hit
ACCGGGTGCTGTAGGCTTT423170.5713456332570495No Hit
TATTGCACTTGTCCCGGCCTGTA421780.5694689160270302No Hit
TTCAAGTAATTCAGGATAGGTT420490.5677272144250697No Hit
TTCTCACTACTGCACTTGACTAG385080.5199181805293962No Hit
CATTGCACTTGTCTCGGTCTGA372190.502514666072598No Hit
TTAATGCTAATCGTGATAGGGGTT367940.4967765018800926No Hit
TGTAAACATCCTTGACTGGAAGCT323190.4363570083237135No Hit
TGAGGTAGTAGTTTGTGCTGTT320120.4322120285423038No Hit
CCCATAAAGTAGAAAGCACT306470.41378239531225736No Hit
AGCAGCATTGTACAGGGCTATGA301450.4070046107836982No Hit
CAACGGAATCCCAAAAGCAGCTGT301070.4064915513970742No Hit
CAACACCAGTCGATGGGCTGT293430.3961763574133706No Hit
ACCGGGTGCTGTAGGCTTA282940.38201321802998695No Hit
CAACGGAATCCCAAAAGCAGCT267400.3610317894296265No Hit
CCCATAAAGTAGAAAGCACTAC230460.31115701642465116No Hit
AACGGAATCCCAAAAGCAGCTG228840.3089697632500962No Hit
CTCCCACTGCTTCACTTGACTAG215490.29094517690422667No Hit
TGAGGTAGTAGATTGTATAGT211400.2854230377166157No Hit
TGAGAACTGAATTCCATAGGCTGT190510.2572182730151015No Hit
ACTGGACTTGGAGTCAGAAGGC190110.2566782105028657No Hit
TCGTTTCCCGGCCAATGCACCA186710.2520876791488615No Hit
TGTAAACATCCCCGACTGGAAGCT172020.23225388338700206No Hit
CCCCCACTGCTAAATTTGACTGGCTT167070.22557060979808413No Hit
TCCTGTCTGAGCGTCGCT161890.2185768002646306No Hit
TGATGTAGTAGATTGTATAGTT154720.20889617973280405No Hit
CTTCTCACTACTGCACTTGACTAG151480.20452167338369415No Hit
TAGCAGCACGTAAATATTGGC147430.19905354044730678No Hit
CCCATAAAGTAGAAAGCACTA147340.1989320263820537No Hit
TTCACAGTGGCTAAGTTCTG140070.1891163902221682No Hit
TTCACAGTGGCTAAGTTCCGC138520.18702364798725452No Hit
CCCCCCACTGCTAAATTTGACTGG134070.18101545253863136No Hit
TGTAAACATCCCCGACTGGA120890.16322039276046202No Hit
TTCACAGTGGCTAAGTTCCG118200.15958847236567633No Hit
TTCACAGTGGCTAAGTTCTGC118100.15945345673761738No Hit
TAGCTTATCAGACTGATGTTGACA116740.1576172441960157No Hit
TGAGGTAGTAAGTTGTATTGTT114830.1550384457000898No Hit
TGGCTCAGTTCAGCAGGAAC110330.14896274243743712No Hit
CACCCGGCTGTGTGCACATGTGC109450.1477746049105184No Hit
GGAATACCGGGTGCTGTAGGCTTT108910.14704552051900008No Hit
CACTAGATTGTGAGCTCCTGGA107100.14460173765113313No Hit
AGCTACATTGTCTGCTGGGTTT105860.14292754386320217No Hit
TTCAAGTAATCCAGGATAGGC104670.14132085788930068No Hit
CAAAGAATTCTCCTTTTGGGCT103860.14022723130202322No Hit
ACCCCCCACTGCTAAATTTGACTG101070.13646029527917858No Hit
TATTGCACTTGTCCCGGCCTGTT100950.13629827652550783No Hit
TCCCACTGCTTCACTTGACTAG96350.13008755763479624No Hit
TGTAAACATCCTACACTCTCAGCT96100.12975001856464888No Hit
GACCCGCGGGCGCTCTCCAGTCCT95010.12827834821880635No Hit
GGAATACCGGGTGCTGTAGGCTT93000.12556453409482146No Hit
TGAGGTAGTAGTTTGTGCTGTTA89740.12116302462009977No Hit
TGAGAACTGAATTCCATGGGTTG89020.12019091209807535No Hit
TGTCAGTTTGTCAAATACCCCA88540.1195428370833924No Hit
ATCCCACCGCTGCCACCA87280.11784164016984966No Hit
AGCTCGGTCTGAGGCCCCTCAGT86500.11678851827098986No Hit
TTATAAAGCAATGAGACTGATT84900.11462826822204669No Hit
TGATGTAGTAGGTTGTATAGTT83260.11241401192187996No Hit
CTCCCACTGCTTCACTTGACTAGCCT82780.11176593690719701No Hit
TGATAACTGAATTCCATGGGTT78400.1058522523982151No Hit
CCCCCCACTGCTAAATTTTACTG78300.10571723677015614No Hit
CACTAGATTGTGAGCTCCTGGAA75180.10150474917471697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)