FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9289014
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8363449.003582080939914No Hit
CAACGGAATCCCAAAAGCAGCTG4806165.174026005343516No Hit
AACATTCAACGCTGTCGGTGAGT3659003.939061777708592No Hit
CCCCCACTGCTAAATTTGACTG3225333.4721984486189816No Hit
AAGCTGCCAGTTGAAGAACTGT3064613.2991768555844567No Hit
TTCAAGTAATCCAGGATAGGCT2987573.216240173607231No Hit
TAGCAGCACGTAAATATTGGCG2499032.6903070659598534No Hit
AACATTCAACGCTGTCGGTGAG1939542.087993408127063No Hit
TAGCTTATCAGACTGATGTTGAC1425661.534780763598806No Hit
TAGCTTATCAGACTGATGTTGA1392161.4987166560412117No Hit
CGCCTGTCTGAGCGTCGCT1370721.4756356272043514No Hit
GCCTGTCTGAGCGTCGCT1365381.4698869008056183No Hit
TGAGGTAGTAGGTTGTATAGTT1293951.3929896111686344No Hit
TAGCTTATCAGACTGATGTTGACT1211231.3039381790144788No Hit
CATAAAGTAGAAAGCACTACT1055301.1360732150904285No Hit
TATTGCACTTGTCCCGGCCTGTAA826180.8894162502069649No Hit
TATTGCACTTGTCCCGGCCTGT820740.8835598697558212No Hit
TGAGGTAGTAGATTGTATAGTT790400.8508976302544059No Hit
TGTAAACATCCTTGACTGGAAGCT734940.7911926927874154No Hit
TATTGCACTTGTCCCGGCCTGTAT667140.7182032452529408No Hit
TCCTGTCTGAGCGTCGCT542130.5836249143342879No Hit
AGCAGCATTGTACAGGGCTATGA518730.5584338660701771No Hit
TATTGCACTTGTCCCGGCCTGTA500780.5391099636624511No Hit
TCGTACGACTCTTAGCGG485390.5225420049964399No Hit
ACTGGACTTGGAGTCAGAAGGC477250.5137789651302065No Hit
TTCAAGTAATTCAGGATAGGTT476220.512670128390376No Hit
ACCGGGTGCTGTAGGCTT451290.4858319731243811No Hit
TACGCCTGTCTGAGCGTCGCT419770.45189941580451914No Hit
CCCATAAAGTAGAAAGCACTAC407610.4388086830313745No Hit
TTCTCACTACTGCACTTGACTAG397020.4274081188810782No Hit
CTACGCCTGTCTGAGCGTCGCT396300.4266330097037209No Hit
TGTAAACATCCCCGACTGGAAGCT384030.4134238574729244No Hit
CCCATAAAGTAGAAAGCACT377510.4064048132557449No Hit
CAACACCAGTCGATGGGCTGT360160.38772683516248335No Hit
ACGCCTGTCTGAGCGTCGCT345250.37167561594804355No Hit
TGAGGTAGTAGTTTGTACAGTT337640.363483142559587No Hit
ACCGGGTGCTGTAGGCTTT328530.3536758583849696No Hit
CATTGCACTTGTCTCGGTCTGA327450.3525131946189337No Hit
ACCGGGTGCTGTAGGCTTA296410.31909737675064326No Hit
AACATTCAACGCTGTCGGTGAGTT288710.3108080147150171No Hit
TTCACAGTGGCTAAGTTCCG267560.2880391826301478No Hit
CAACGGAATCCCAAAAGCAGCTGT260860.28082636111862896No Hit
TGTCAGTTTGTCAAATACCCCAA257750.27747832008865525No Hit
AACCGGGCGGAAACACCA251110.27033009100858285No Hit
CTCCCACTGCTTCACTTGACTAG250970.2701793753352078No Hit
TGAGGTAGTAGTTTGTGCTGTT236500.25460183395137526No Hit
TGTAAACATCCTACACTCTCAGCT236400.2544941798989645No Hit
CTTCTCACTACTGCACTTGACTAG225330.24257687629709676No Hit
GACCCGCGGGCGCTCTCCAGTCCT219420.23621452179962266No Hit
ATCCCGGACGAGCCCCCA217800.23447052615056885No Hit
GCGTTCGCGCTTTCCCCTG175240.18865296144456237No Hit
TTATAAAGCAATGAGACTGATT172910.18614462202339235No Hit
CCCCCCACTGCTAAATTTGACTGG164410.17699402756848034No Hit
TAGCTTATCAGACTGATGTTGACA164150.17671412703221245No Hit
TTCACAGTGGCTAAGTTCTG157890.1699749833513008No Hit
CCCCCACTGCTAAATTTGACTGGCTT153360.16509825477709475No Hit
TTCAAGTAATCCAGGATAGGC150610.16213776833579968No Hit
CAAAGAATTCTCCTTTTGGGCT147700.15900503541064745No Hit
TGTCAGTTTGTCAAATACCCCA145440.15657205382616496No Hit
TGAGGTAGTAGGTTGTATAGT143660.154655811693254No Hit
ATCCCACCGCTGCCACCA142770.1536976906267985No Hit
AACGGAATCCCAAAAGCAGCTG142040.15291181604420018No Hit
TTCAAGTAATCCAGGATAGGCTT141750.15259961929220905No Hit
TGAGGTAGTAGGTTGTGTGGTT141530.15236278037690543No Hit
ACTGGACTTGGAGTCAGAAGG137040.14752911342366368No Hit
CAACGGAATCCCAAAAGCAGCT132660.14281386592807374No Hit
TATTGCACTTGTCCCGGCCTGTT130410.14039164974883234No Hit
ACTGGACTTGGAGTCAGAAGGCA128910.1387768389626714No Hit
TAGCAGCACGTAAATATTGGC128140.13794790275910876No Hit
GGAATACCGGGTGCTGTAGGCTTT126780.13648380764632284No Hit
AACATTCAACGCTGTCGGTGA126460.13613931467860851No Hit
CAAAGTGCTGTTCGTGCAGGT125450.13505200874926016No Hit
ACCACAGGGTAGAACCACGGAC123800.13327571688448311No Hit
TGTAAACATCCCCGACTGGAAGC123370.13281280445911697No Hit
CTCGACACAAGGGTTTGT121760.13107957421530425No Hit
TTCACAGTGGCTAAGTTCTGC120900.13015374936457194No Hit
TGTAAACATCCTACACTCAGCT119590.1287434812779914No Hit
CCCCCCACTGCTAAATTTGACTGGCT118720.12780689102201806No Hit
AGCAGCATTGTACAGGGCTATG111710.12026034194802591No Hit
ACTGGACTTGGAGTCAGAAGGCT108850.11718143604907906No Hit
CAAAGAATTCTCCTTTTGGGCTT108340.11663240038178432No Hit
CACTAGATTGTGAGCTCCTGGA107490.11571734093629314No Hit
AACATTCAACGCTGTCGGTGAGA106600.11475921986983763No Hit
CCTCGACACAAGGGTTTGT104360.11234776909583728No Hit
TTCACAGTGGCTAAGTTCCGC102990.1108729085778103No Hit
ACCCCCCACTGCTAAATTTGACTG101850.10964565238032799No Hit
AGCTCGGTCTGAGGCCCCTCAGT101750.10953799832791726No Hit
GGAATACCGGGTGCTGTAGGCTT101570.10934422103357794No Hit
AGAGGTAGTAGGTTGCATAGTT100870.10859064266670283No Hit
AGCAGCATTGTACAGGGCTATGT98480.10601771081408641No Hit
TAGCAGCACGTAAATATTGGCGT96530.10391845679207717No Hit
TGAGGTAGTAGATTGTATAGT94900.10216369573778229No Hit
CCCATAAAGTAGAAAGCACTA94720.10196991844344297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)