FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10655077
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6576376.172053003464921No Hit
CCCCCCACTGCTAAATTTGACTG5090374.777412683174416No Hit
TAGCAGCACGTAAATATTGGCG3585683.3652314291112115No Hit
AAGCTGCCAGTTGAAGAACTGT3532263.3150957050803105No Hit
AACATTCAACGCTGTCGGTGAGT3295193.0926008324482313No Hit
TTCAAGTAATCCAGGATAGGCT3198153.0015268777503907No Hit
TAGCTTATCAGACTGATGTTGAC2655802.492520701633597No Hit
AACATTCAACGCTGTCGGTGAG1844461.731062103070677No Hit
TAGCTTATCAGACTGATGTTGACT1817191.7054686700058572No Hit
CCCCCACTGCTAAATTTGACTG1547851.4526877656538757No Hit
TATTGCACTTGTCCCGGCCTGTAA1395261.3094790398980694No Hit
TAGCTTATCAGACTGATGTTGA1376151.291543927838344No Hit
TGTAAACATCCTTGACTGGAAGCT1323771.2423842643277003No Hit
TATTGCACTTGTCCCGGCCTGTAT1288751.2095173033475026No Hit
CGCCTGTCTGAGCGTCGCT1251691.1747357621160315No Hit
GCCTGTCTGAGCGTCGCT1155971.0849006534631331No Hit
AGCAGCATTGTACAGGGCTATGA999190.9377595300343676No Hit
TATTGCACTTGTCCCGGCCTGT970460.9107958581622638No Hit
TGAGGTAGTAGGTTGTATAGTT959810.9008006230269382No Hit
ACTGGACTTGGAGTCAGAAGGC832260.7810924313357848No Hit
TTCAAGTAATTCAGGATAGGTT785930.7376108121977908No Hit
CATAAAGTAGAAAGCACTACT742860.6971887673829105No Hit
TGAGGTAGTAGATTGTATAGTT734010.6888828677634146No Hit
TATTGCACTTGTCCCGGCCTGTA671640.6303473921399161No Hit
TGTAAACATCCCCGACTGGAAGCT620720.5825579674365563No Hit
TACGCCTGTCTGAGCGTCGCT538950.5058152090313378No Hit
TCCTGTCTGAGCGTCGCT525610.49329535581957784No Hit
TTCTCACTACTGCACTTGACTAG443090.415848707616097No Hit
CTACGCCTGTCTGAGCGTCGCT416140.3905556008652026No Hit
TGTCAGTTTGTCAAATACCCCAA397900.37343700097146176No Hit
CAACGGAATCCCAAAAGCAGCTGT396970.3725641776216164No Hit
CCCATAAAGTAGAAAGCACTAC394090.36986124079628896No Hit
CTTCTCACTACTGCACTTGACTAG389850.36588191713677903No Hit
TGTAAACATCCTACACTCTCAGCT359020.3369473538295406No Hit
CATTGCACTTGTCTCGGTCTGA355420.3335686827978812No Hit
GCGTTCGCGCTTTCCCCTG351620.33000230782001855No Hit
AACATTCAACGCTGTCGGTGAGTT333410.31291186351820827No Hit
CCCCCACTGCTAAATTTGACTGGCTT327530.3073933674998313No Hit
AACCGGGCGGAAACACCA319580.2999321356382502No Hit
CGCGACCTCAGATCAGACGTGGCG304770.28603265842189596No Hit
CACCCGGCTGTGTGCACATGTGC302880.2842588561302748No Hit
ACGCCTGTCTGAGCGTCGCT282140.26479395690899277No Hit
TGAGGTAGTAGTTTGTACAGTT273550.2567320724195611No Hit
TAGCTTATCAGACTGATGTTGACA264500.24823846885386186No Hit
CAACACCAGTCGATGGGCTGT261200.24514135374150747No Hit
CCCCCCACTGCTAAATTTGACTGGCT253450.23786782582612964No Hit
TGTCAGTTTGTCAAATACCCCA241980.22710300451137053No Hit
TTCAAGTAATCCAGGATAGGCTT230740.21655404273474513No Hit
CCCATAAAGTAGAAAGCACT230420.21625371642081986No Hit
TTATAAAGCAATGAGACTGATT221410.20779765364436126No Hit
ACTGGACTTGGAGTCAGAAGGCA213660.20052412572898348No Hit
CAACGGAATCCCAAAAGCAGCT210830.1978681148902068No Hit
TGAGGTAGTAGTTTGTGCTGTT210720.19776487771979498No Hit
TAGCAGCACGTAAATATTGGC207950.1951651780648793No Hit
CTCCCACTGCTTCACTTGACTAG207750.194977474118676No Hit
AGCAGCATTGTACAGGGCTATGT205190.1925748636072738No Hit
TAGCAGCACGTAAATATTGGCGT204850.1922557668987282No Hit
CAAAGAATTCTCCTTTTGGGCTT194060.18212913900106023No Hit
TCGTACGACTCTTAGCGG187040.17554073048932448No Hit
CCCCCCACTGCTAAATTTGACTGG186410.1749494630587841No Hit
TGTAAACATCCTACACTCAGCT183450.17217144465497528No Hit
AACGGAATCCCAAAAGCAGCTG180860.16974067855164257No Hit
CCAAGTCCTTCTGATCGAGGCCC180520.16942158184309694No Hit
CAAAGAATTCTCCTTTTGGGCT178000.1670565121209354No Hit
TATTGCACTTGTCCCGGCCTGTT173020.16238268386047328No Hit
ACTGGACTTGGAGTCAGAAGG171550.16100305985587904No Hit
TCGTTCGCGCTTTCCCCTG170150.15968913223245595No Hit
CACTAGATTGTGAGCTCCTGGA165910.15570980857294603No Hit
TGTAAACATCCCCGACTGGAAGC163150.1531194941153405No Hit
ACTGGACTTGGAGTCAGAAGGCT161090.15118614346944653No Hit
AGCAGCATTGTACAGGGCTATG160020.1501819273572589No Hit
CGCGACCTCAGATCAGACGTGG159270.14947803755899652No Hit
CCTCGACACAAGGGTTTGT155100.14556441028065775No Hit
TATTGCACTTGTCCCGGCCTGTAG155080.14554563988603744No Hit
ACCACAGGGTAGAACCACGGAC151140.14184787214583244No Hit
CAAAGTGCTGTTCGTGCAGGTAG150780.1415100050426665No Hit
CACTAGATTGTGAGCTCCTGGAA146150.13716465868806016No Hit
GGAATACCGGGTGCTGTAGGCTTT143400.1345837294277648No Hit
CAAGTCCTTCTGATCGAGGCCC142760.13398307679991425No Hit
TTCAAGTAATCCAGGATAGGC141750.13303517187158762No Hit
CTGCGTTCGCGCTTTCCCCTG141090.13241574884911672No Hit
TTCACAGTGGCTAAGTTCCG138360.1298535899834417No Hit
TTCACAGTGGCTAAGTTCTGC134220.12596811829703344No Hit
TTCACAGTGGCTAAGTTCCGC133220.12502959856601695No Hit
GGGCTACGCCTGTCTGAGCGTCGCT133080.12489820580367463No Hit
ACCGGGTGCTGTAGGCTT130000.12200756503214383No Hit
ATCCCACCGCTGCCACCA127820.1199615920185279No Hit
AGCTCGGTCTGAGGCCCCTCAGT123520.11592595717515697No Hit
ATAGGCGATAGAAATTGAAACCT119460.11211556706723003No Hit
TACCTGGTTGATCCTGCC116460.10930000787418054No Hit
CTCCCACTGCTTCACTTGACTAGCCT112710.10578055888286869No Hit
CAAAGTGCTGTTCGTGCAGGT112470.10555531414742474No Hit
ATCACATTGCCAGGGATTTCCA111110.1042789273132423No Hit
GGAATACCGGGTGCTGTAGGCTT107940.10130381976592004No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT107880.10124750858205905No Hit
TGTAAACATCCTTGACTGGAAGC107810.1011818122008879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)