FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11103928
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6952496.261288797982119No Hit
AAGCTGCCAGTTGAAGAACTGT4099283.6917386351928796No Hit
TTCAAGTAATCCAGGATAGGCT3555403.2019299836958592No Hit
AACATTCAACGCTGTCGGTGAGT3147162.8342763029443274No Hit
TAGCAGCACGTAAATATTGGCG3146572.8337449594413795No Hit
CCCCCCACTGCTAAATTTGACTG3051972.7485498825280565No Hit
TAGCTTATCAGACTGATGTTGAC1942161.7490747418391042No Hit
AACATTCAACGCTGTCGGTGAG1733531.5611862757035169No Hit
CGCCTGTCTGAGCGTCGCT1639611.4766035946918963No Hit
GCCTGTCTGAGCGTCGCT1565321.4096993424308948No Hit
TAGCTTATCAGACTGATGTTGACT1348211.2141739391681934No Hit
TAGCTTATCAGACTGATGTTGA1327711.195712003896279No Hit
TATTGCACTTGTCCCGGCCTGT1278511.1514033592436839No Hit
TATTGCACTTGTCCCGGCCTGTAA1253741.1290959379419607No Hit
TATTGCACTTGTCCCGGCCTGTAT1196471.0775195948676901No Hit
CCCCCACTGCTAAATTTGACTG1097340.9882448805503782No Hit
TGAGGTAGTAGGTTGTATAGTT1067820.9616596937588212No Hit
TGTAAACATCCTTGACTGGAAGCT1010710.9102274438378923No Hit
CATAAAGTAGAAAGCACTACT981680.8840835423284445No Hit
TTCAAGTAATTCAGGATAGGTT875970.7888829970799522No Hit
AGCAGCATTGTACAGGGCTATGA824670.7426831297897465No Hit
TGAGGTAGTAGATTGTATAGTT772830.6959969481070122No Hit
TATTGCACTTGTCCCGGCCTGTA687220.6188981052470801No Hit
TTCTCACTACTGCACTTGACTAG594860.535720332480542No Hit
ACTGGACTTGGAGTCAGAAGGC565830.5095764309710942No Hit
CTACGCCTGTCTGAGCGTCGCT548340.4938252481464217No Hit
TCGTACGACTCTTAGCGG520050.46834777747117956No Hit
TGTAAACATCCCCGACTGGAAGCT503560.45349717685489316No Hit
CCCATAAAGTAGAAAGCACTAC477660.43017209765769376No Hit
TACGCCTGTCTGAGCGTCGCT469610.42292241088018584No Hit
CAACGGAATCCCAAAAGCAGCTGT450840.4060184828287791No Hit
CATTGCACTTGTCTCGGTCTGA423680.3815586700490133No Hit
AACCGGGCGGAAACACCA418290.3767045319458123No Hit
ACGCCTGTCTGAGCGTCGCT408530.36791484959196424No Hit
CTTCTCACTACTGCACTTGACTAG386520.34809303518538665No Hit
ATCCCACCGCTGCCACCA350840.3159602619901714No Hit
TGTAAACATCCTACACTCTCAGCT327640.29506675475561445No Hit
CAACACCAGTCGATGGGCTGT327630.29505774893353054No Hit
GCGTTCGCGCTTTCCCCTG326230.29379693384179006No Hit
CCCATAAAGTAGAAAGCACT318920.28721367789848784No Hit
CACCCGGCTGTGTGCACATGTGC315880.2844759079849941No Hit
CCCCCACTGCTAAATTTGACTGGCTT313750.2825576678811318No Hit
TGAGGTAGTAGTTTGTACAGTT307400.2768389708578802No Hit
TTAATGCTAATCGTGATAGGGGTT303290.2731375779814134No Hit
GGAATACCGGGTGCTGTAGGCTTT298950.26922905119701784No Hit
CTCCCACTGCTTCACTTGACTAG293930.26470812851091974No Hit
ACCGGGTGCTGTAGGCTT287050.25851212291722353No Hit
AACATTCAACGCTGTCGGTGAGTT279490.2517037214218248No Hit
ATCCCGGACGAGCCCCCA272330.24525555280978045No Hit
TGTCAGTTTGTCAAATACCCCAA252370.2272799319303944No Hit
AGCTCGGTCTGAGGCCCCTCAGT244290.22000322768663486No Hit
TGAGGTAGTAGTTTGTGCTGTT232760.20961951482394336No Hit
GGAATACCGGGTGCTGTAGGCTT226900.20434210308280096No Hit
CCCCCCACTGCTAAATTTGACTGGCT218930.19716446288196393No Hit
ACCGGGTGCTGTAGGCTTA216560.19503008304808891No Hit
ACCGGGTGCTGTAGGCTTT205820.18535783013002247No Hit
GGGCTACGCCTGTCTGAGCGTCGCT205030.18464637018539745No Hit
TTCAAGTAATCCAGGATAGGCTT203190.18298929892196705No Hit
TATTGCACTTGTCCCGGCCTGTT200620.18067480264641486No Hit
TAGCTTATCAGACTGATGTTGACA200400.1804766745605699No Hit
TGTCAGTTTGTCAAATACCCCA197600.1779550443770889No Hit
CGCGACCTCAGATCAGACGTGGCG195880.17640604297866486No Hit
TAGCAGCACGTAAATATTGGC195080.175685577211956No Hit
TAGCAGCACGTAAATATTGGCGT194390.17506417548816958No Hit
AGCAGCATTGTACAGGGCTATGT190360.17143482918837372No Hit
TTCACAGTGGCTAAGTTCCG185040.16664373183975978No Hit
TGATGTAGTAGGTTGTATAGTT183740.16547297496885788No Hit
TTCAAGTAATCCAGGATAGGC180840.16286128656453824No Hit
TTATAAAGCAATGAGACTGATT178080.16037567966939267No Hit
TGTAAACATCCTACACTCAGCT175280.15785404948591167No Hit
TTCACAGTGGCTAAGTTCCGC174300.1569714789216933No Hit
TTCACAGTGGCTAAGTTCTGC173490.15624200733290058No Hit
AACGGAATCCCAAAAGCAGCTG163270.14703805716319487No Hit
AGCAGCATTGTACAGGGCTATG161640.14557010816352556No Hit
ACCACAGGGTAGAACCACGGAC159490.14363385641549548No Hit
CAACGGAATCCCAAAAGCAGCT157270.1416345639128784No Hit
CAAAGTGCTGTTCGTGCAGGTAG157230.14159854062454294No Hit
TACCTGGTTGATCCTGCC155270.13983339949610624No Hit
CCCCCCACTGCTAAATTTGACTGG154440.1390859162631458No Hit
TGTAAACATCCCCGACTGGAAGC149750.1348621857058151No Hit
CCTCGACACAAGGGTTTGT146140.13161108393354135No Hit
CGCGACCTCAGATCAGACGTGG145680.13119681611768377No Hit
CAAAGTGCTGTTCGTGCAGGT143680.1293956517009116No Hit
CAAAGAATTCTCCTTTTGGGCT143500.1292335469034021No Hit
TATTGCACTTGTCCCGGCCTGTAG142740.1285491044250287No Hit
ACTGGACTTGGAGTCAGAAGG136140.1226052618496806No Hit
TGATGTAGTAGATTGTATAGTT133850.12054292859247646No Hit
TGAGGTAGTAGGTTGTGTGGTT129110.11627416892472646No Hit
CAAAGAATTCTCCTTTTGGGCTT127830.11512142369799226No Hit
CTCCCACTGCTTCACTTGACTAGCCT127110.1144730045079543No Hit
ACTGGACTTGGAGTCAGAAGGCA124560.11217651987656979No Hit
CCAAGTCCTTCTGATCGAGGCCC124190.11184330445946696No Hit
GTCAGGATGGCCGAGCGGTCTAAGG124110.11177125788279607No Hit
AGAGGTAGTAGGTTGCATAGTT123100.11086166985232614No Hit
TTCACCACCTTCTCCACCCAGC122880.11066354176648119No Hit
CACTAGATTGTGAGCTCCTGGA121960.10983500613476602No Hit
ACTGGACTTGGAGTCAGAAGGCT121950.10982600031268215No Hit
CTCGACACAAGGGTTTGT116580.1049898738536489No Hit
TGAGGTAGTAGGTTGTATAGT112180.10102731213675016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)