FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6838788
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG111334316.27982911591937No Hit
CCCCCCACTGCTAAATTTGACTG3310654.840989368291575No Hit
TTCAAGTAATCCAGGATAGGCT2404783.5163833123647055No Hit
TGAGGTAGTAGATTGTATAGTT1625662.3771171149039856No Hit
TAGCAGCACGTAAATATTGGCG1558402.2787663545060908No Hit
AACATTCAACGCTGTCGGTGAG1489382.1778420386770287No Hit
TCGTACGACTCTTAGCGG1466502.144385818071857No Hit
CCCCCACTGCTAAATTTGACTG1411852.0644739974393125No Hit
TAGCTTATCAGACTGATGTTGAC1249841.827575295505578No Hit
AAGCTGCCAGTTGAAGAACTGT1189281.7390215927149664No Hit
TGAGAACTGAATTCCATAGGCTGT955571.3972797519092564No Hit
TAGCTTATCAGACTGATGTTGA934921.367084343015166No Hit
TGAGAACTGAATTCCATGGGTT903641.321345244215788No Hit
TAGCTTATCAGACTGATGTTGACT794461.1616970726391869No Hit
TATTGCACTTGTCCCGGCCTGTAA786391.1498967360883243No Hit
TATTGCACTTGTCCCGGCCTGT766591.1209442374876952No Hit
TATTGCACTTGTCCCGGCCTGTAT741751.084622011970542No Hit
TGAGGTAGTAGGTTGTATAGTT693621.014244044412548No Hit
TTCAAGTAATTCAGGATAGGTT686481.0038035979474726No Hit
GCCTGTCTGAGCGTCGCT580020.8481327393099478No Hit
TTCTCACTACTGCACTTGACTAG489130.7152290727538271No Hit
TGAGGTAGTAGTTTGTACAGTT488570.7144102142075467No Hit
CACCCGGCTGTGTGCACATGTGC460160.672867765457856No Hit
TTAATGCTAATCGTGATAGGGGTT455240.6656735082298209No Hit
CAACGGAATCCCAAAAGCAGCTGT447170.6538731716789583No Hit
TGTAAACATCCTTGACTGGAAGCT443590.6486383259723799No Hit
CATAAAGTAGAAAGCACTACT427230.624715958441759No Hit
TATTGCACTTGTCCCGGCCTGTA403310.5897390005363523No Hit
AACATTCAACGCTGTCGGTGAGT399380.5839923682383487No Hit
CCCCCACTGCTAAATTTGACTGGCTT350660.5127516747119518No Hit
AGCAGCATTGTACAGGGCTATGA343860.502808392364261No Hit
CAACGGAATCCCAAAAGCAGCT333290.48735243730321803No Hit
CATTGCACTTGTCTCGGTCTGA328410.4802166699713458No Hit
CTTCTCACTACTGCACTTGACTAG268930.39324219437713237No Hit
AACGGAATCCCAAAAGCAGCTG259150.3789414147653064No Hit
CCCATAAAGTAGAAAGCACTAC255260.3732532723634656No Hit
TGATGTAGTAGATTGTATAGTT242710.3549020674423597No Hit
TGAGGTAGTAGTTTGTGCTGTT236200.3453828368418497No Hit
CCCATAAAGTAGAAAGCACT234770.34329182305402656No Hit
TGTAAACATCCCCGACTGGAAGCT224970.32896179849411916No Hit
ACTGGACTTGGAGTCAGAAGGC220180.32195763342861333No Hit
TCGTTTCCCGGCCAATGCACCA203000.29683622302665325No Hit
TTCACAGTGGCTAAGTTCCGC195870.2864103990356186No Hit
CTCCCACTGCTTCACTTGACTAG195130.2853283359566052No Hit
TGAGGTAGTAGATTGTATAGT174490.2551475495365553No Hit
TAGCAGCACGTAAATATTGGC164180.24007177880057112No Hit
ACCGGGTGCTGTAGGCTT160540.23474919824974833No Hit
CAACACCAGTCGATGGGCTGT159720.23355015537840917No Hit
GGAATACCGGGTGCTGTAGGCTTT149630.21879607907132081No Hit
CTCCCACTGCTTCACTTGACTAGCCT142440.20828252023604182No Hit
TAGCTTATCAGACTGATGTTGACA140200.20500708605092013No Hit
TGTAAACATCCTACACTCTCAGCT137950.20171702939175773No Hit
TTCACAGTGGCTAAGTTCTGC137150.20054723146849998No Hit
TGTCAGTTTGTCAAATACCCCA137070.20043025167617418No Hit
CAAAGAATTCTCCTTTTGGGCT133480.19518078349555504No Hit
TGATAACTGAATTCCATAGGCTGT130570.190925643549705No Hit
CCCATAAAGTAGAAAGCACTA128590.18803039368964208No Hit
CACTAGATTGTGAGCTCCTGGA127810.18688984071446577No Hit
GGAATACCGGGTGCTGTAGGCTT124910.18264932324265645No Hit
TGATAACTGAATTCCATGGGTT122610.17928615421329042No Hit
TGAGAACTGAATTCCATGGGTTG120060.17555742333290636No Hit
CACCCGTAGAACCGACCTTGCG119420.17462158499430017No Hit
TGTAAACATCCCCGACTGGA116490.17033720010036865No Hit
CCCCCCACTGCTAAATTTGACTGGCT113420.16584810056986704No Hit
CACTAGATTGTGAGCTCCTGGAA113200.16552640614097117No Hit
CCCCCCACTGCTAAATTTGACTGG109610.16027693796035206No Hit
TGGCTCAGTTCAGCAGGAAC108620.15882931303032058No Hit
ACCGGGTGCTGTAGGCTTT107700.15748404541857416No Hit
TTAATGCTAATCGTGATAGGGGTTT103950.1520006176533035No Hit
TGATGTAGTAGGTTGTATAGTT103620.15151807600995967No Hit
TCGTTTCCCGGCCAACGCACCA102660.15011431850205037No Hit
CAAAGTGCTGTTCGTGCAGGTAG102380.14970488922891015No Hit
TTCAAGTAATCCAGGATAGGC99580.14561059649750804No Hit
TATTGCACTTGTCCCGGCCTGTT99260.14514267732820493No Hit
AATCCTTGGAACCTAGGTGTGAGT98870.1445724008406168No Hit
TATTGCACTTGTCCCGGCCTGTAG98060.14338798044331832No Hit
ACCCCCCACTGCTAAATTTGACTG96250.14074131264194767No Hit
ATGCACCTGGGCAAGGATTCTGA91650.13401497458321562No Hit
ACCGGGTGCTGTAGGCTTA91090.13319611603693518No Hit
AGCTCGGTCTGAGGCCCCTCAGT89590.13100274493082692No Hit
TTCACAGTGGCTAAGTTCTG87420.1278296680639903No Hit
ATCACATTGCCAGGGATTTCC86910.12708392188791348No Hit
TTCAAGTAATCCAGGATAGGCTT86060.1258410115944521No Hit
TAGCAGCACATCATGGTTTACA83170.1216151165966835No Hit
TATTGCACTTGTCCCGGCCTGTAC82550.12070852320615875No Hit
TTATAAAGCAATGAGACTGATT81860.11969957249734894No Hit
TCCCACTGCTTCACTTGACTAG81450.11910005106167934No Hit
ACTGGACTTGGAGTCAGAAGGCT81040.11850052962600974No Hit
TGAGGTAGTAAGTTGTATTGTT78980.11548829997362106No Hit
TGAGGTAGTAGATTGTATAGTTA77470.11328030639347206No Hit
CAAAGAATTCTCCTTTTGGGCTT76880.11241758042506947No Hit
TGAGGTAGTAGTTTGTGCTGTTA73900.10806008316093436No Hit
AGCAGCATTGTACAGGGCTATGT72760.10639312112029208No Hit
TGATGTAGTAGTTTGTACAGTT71850.10506247598258639No Hit
CAAAGAATTCTCCTTTTGGGC71420.10443370959883536No Hit
TGTCAGTTTGTCAAATACCCCAA68500.10016394717894457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)