FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8473687
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7577598.94249457172539No Hit
CCCCCCACTGCTAAATTTGACTG5646986.6641356944149575No Hit
TTCAAGTAATCCAGGATAGGCT3020743.564847273683817No Hit
AACATTCAACGCTGTCGGTGAGT2243682.6478202463697325No Hit
CCCCCACTGCTAAATTTGACTG2080092.454763788183349No Hit
TAGCAGCACGTAAATATTGGCG1820342.1482266220123543No Hit
AAGCTGCCAGTTGAAGAACTGT1726142.037058956744567No Hit
AACATTCAACGCTGTCGGTGAG1388581.6386963549633118No Hit
TCGTACGACTCTTAGCGG914981.079789706653078No Hit
CATAAAGTAGAAAGCACTACT868931.0254450040460545No Hit
TAGCTTATCAGACTGATGTTGA797510.9411605597421759No Hit
TAGCTTATCAGACTGATGTTGAC687670.8115357576931979No Hit
TATTGCACTTGTCCCGGCCTGTAA669340.7899040877955488No Hit
TATTGCACTTGTCCCGGCCTGT664250.7838972574748159No Hit
TATTGCACTTGTCCCGGCCTGTAT654180.7720134104552128No Hit
TTCAAGTAATTCAGGATAGGTT593020.699837036699609No Hit
TAGCTTATCAGACTGATGTTGACT541700.6392730814815322No Hit
ACCGGGTGCTGTAGGCTTA533340.6294072462199749No Hit
CGCCTGTCTGAGCGTCGCT519980.6136407917828449No Hit
AGCAGCATTGTACAGGGCTATGA486810.5744960841720965No Hit
CCCATAAAGTAGAAAGCACTAC483200.5702358371273331No Hit
ATCCCGGACGAGCCCCCA477320.5632967089768598No Hit
ACTGGACTTGGAGTCAGAAGGC452620.5341476502495313No Hit
CAACGGAATCCCAAAAGCAGCTGT451760.5331327437513328No Hit
ACCGGGTGCTGTAGGCTT425660.502331511654844No Hit
TTCTCACTACTGCACTTGACTAG417700.4929377259273325No Hit
CATTGCACTTGTCTCGGTCTGA402070.4744923903844926No Hit
CCCATAAAGTAGAAAGCACT400970.47319425416586663No Hit
CACCCGGCTGTGTGCACATGTGC396860.46834394520354594No Hit
TATTGCACTTGTCCCGGCCTGTA390250.4605433266534391No Hit
CAACACCAGTCGATGGGCTGT384850.4541706579438207No Hit
TGAGGTAGTAGGTTGTATAGTT379650.4480340140012252No Hit
CCCCCACTGCTAAATTTGACTGGCTT362170.4274054493634235No Hit
CTACGCCTGTCTGAGCGTCGCT350690.41385762773630885No Hit
TGAGGTAGTAGATTGTATAGTT347080.4095973806915455No Hit
CTCCCACTGCTTCACTTGACTAG333490.39355949777233923No Hit
ACCGGGTGCTGTAGGCTTT329030.3882961454677285No Hit
CTTCTCACTACTGCACTTGACTAG320330.37802906810223225No Hit
GCCTGTCTGAGCGTCGCT311190.3672427362492856No Hit
CAACGGAATCCCAAAAGCAGCT307840.363289321401652No Hit
TGTAAACATCCTTGACTGGAAGCT265900.3137949277569493No Hit
CCCCCCACTGCTAAATTTGACTGGCT254830.3007309569022316No Hit
CACTAGATTGTGAGCTCCTGGA241180.28462226655291845No Hit
TTAATGCTAATCGTGATAGGGGTT216690.255721033830964No Hit
AACCGGGCGGAAACACCA214050.2526055069062617No Hit
AGCTCGGTCTGAGGCCCCTCAGT213550.25201544498870443No Hit
TACGCCTGTCTGAGCGTCGCT209980.24780240289734562No Hit
TTCACAGTGGCTAAGTTCCG208050.24552476389557462No Hit
CCCCCCACTGCTAAATTTGACTGG194030.22897942772726912No Hit
CACTAGATTGTGAGCTCCTGGAA192400.22705582587603246No Hit
TGTAAACATCCCCGACTGGAAGCT189600.22375147913771182No Hit
TGTAAACATCCTACACTCTCAGCT189190.22326762836531489No Hit
AACATTCAACGCTGTCGGTGAGTT188010.22187508223987978No Hit
ATCCCACCGCTGCCACCA183210.21621048783133007No Hit
ACGCCTGTCTGAGCGTCGCT172690.20379558508592538No Hit
CAAAGAATTCTCCTTTTGGGCT161520.1906136018476963No Hit
AACGGAATCCCAAAAGCAGCTG156140.1842645356147802No Hit
TTCAAGTAATCCAGGATAGGCTT147970.17462292388189463No Hit
CCCATAAAGTAGAAAGCACTA136780.16141733816696321No Hit
CGCGACCTCAGATCAGACGT136050.16055584776732962No Hit
ACTGGACTTGGAGTCAGAAGG132150.155953364810383No Hit
TTCAAGTAATCCAGGATAGGC128280.15138628556848985No Hit
ACCACAGGGTAGAACCACGGAC125250.14781051034809287No Hit
ACTGGACTTGGAGTCAGAAGGCT124370.1467720013731921No Hit
TCCTGTCTGAGCGTCGCT121940.14390430045386382No Hit
CGCGACCTCAGATCAGACGTGGC119320.1408123760058638No Hit
CCCCCACTGCTAAATTTGACTGGCT118540.13989187941447448No Hit
CAAAGAATTCTCCTTTTGGGCTT116670.13768504784281033No Hit
CGCGACCTCAGATCAGACGTGGCG116270.13721299830876452No Hit
CAAAGTGCTGTTCGTGCAGGT115860.13672914753636758No Hit
TATTGCACTTGTCCCGGCCTGTT113560.1340148627156042No Hit
ACTGGACTTGGAGTCAGAAGGCA110170.130014242914566No Hit
TCGTTTCCCGGGCGGCGCACCA109460.12917635499163468No Hit
TTCACAGTGGCTAAGTTCTG108300.12780741134290186No Hit
AGCAGCATTGTACAGGGCTATGT107910.1273471630472072No Hit
TCGTTTCCCGGCCAATGCACCA106490.12567138720134458No Hit
TAGCAGCACGTAAATATTGGC105990.12508132528378735No Hit
AGCTACATCTGGCTACTGGGTCTC104420.12322853086265753No Hit
TGTCAGTTTGTCAAATACCCCAA102770.1212813265347186No Hit
TTCACAGTGGCTAAGTTCTGC101090.1192987184917262No Hit
AGCTACATTGTCTGCTGGGTTT100700.11883847019603154No Hit
GGAATACCGGGTGCTGTAGGCTTT99430.11733971292543612No Hit
CAAAGTGCTGTTCGTGCAGGTAG95560.11277263368354294No Hit
ACCCCCCACTGCTAAATTTGACTG95520.11272542873013837No Hit
CGCGACCTCAGATCAGACGTGG94530.11155710613337501No Hit
AGCAGCATTGTACAGGGCTATG93780.11067201325703911No Hit
TTCACAGTGGCTAAGTTCCGC92860.10958629932873376No Hit
CTCCCACTGCTTCACTTGACTAGCCT92730.10943288323016888No Hit
TGAGGTAGTAGTTTGTACAGTT90450.10674220088610778No Hit
TGAGGTAGTAGTTTGTGCTGTT89320.10540866095242837No Hit
TATTGCACTTGTCCCGGCCTGTAG87600.10337884795603142No Hit
TGAGAACTGAATTCCATAGGCTGT85700.10113661266931386No Hit
TGAGAACTGAATTCCATGGGTT85430.10081797923383291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)