FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11220853
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG123035810.96492396790155No Hit
CCCCCCACTGCTAAATTTGACTG11111229.902295306782827No Hit
TTCAAGTAATCCAGGATAGGCT3879843.4577050425667286No Hit
AACATTCAACGCTGTCGGTGAGT3346782.9826431199125416No Hit
TAGCAGCACGTAAATATTGGCG3141702.799876266091357No Hit
CCCCCACTGCTAAATTTGACTG2889732.5753211453710336No Hit
AAGCTGCCAGTTGAAGAACTGT2479482.2097072299227163No Hit
AACATTCAACGCTGTCGGTGAG2228331.9858828914343678No Hit
TATTGCACTTGTCCCGGCCTGTAA1797941.6023202514104766No Hit
TATTGCACTTGTCCCGGCCTGTAT1674301.4921325499941938No Hit
TAGCTTATCAGACTGATGTTGACT1392681.2411534132030781No Hit
TAGCTTATCAGACTGATGTTGAC1375811.2261189055769646No Hit
AACCGGGCGGAAACACCA1356701.2090881147805785No Hit
TCGTACGACTCTTAGCGG1007470.8978550917653052No Hit
TATTGCACTTGTCCCGGCCTGT915880.8162302812451068No Hit
TAGCTTATCAGACTGATGTTGA847500.7552901726811678No Hit
CAACGGAATCCCAAAAGCAGCTGT840110.7487042206149568No Hit
CCCATAAAGTAGAAAGCACTAC760290.677568808717127No Hit
CATAAAGTAGAAAGCACTACT758000.675527965654661No Hit
GCCTGTCTGAGCGTCGCT735440.6554225422969181No Hit
TATTGCACTTGTCCCGGCCTGTA684170.6097308288416219No Hit
AGCAGCATTGTACAGGGCTATGA674090.6007475545753964No Hit
TGTAAACATCCTTGACTGGAAGCT619340.5519544726234271No Hit
TTCAAGTAATTCAGGATAGGTT602150.5366347816872746No Hit
ACTGGACTTGGAGTCAGAAGGC561490.500398677355456No Hit
TGAGGTAGTAGGTTGTATAGTT479330.4271778625029666No Hit
CCCATAAAGTAGAAAGCACT466000.4152981952441584No Hit
CATTGCACTTGTCTCGGTCTGA446280.3977237737630107No Hit
CAACGGAATCCCAAAAGCAGCT443910.395611634873035No Hit
TGTAAACATCCCCGACTGGAAGCT426470.3800691444759146No Hit
TGAGGTAGTAGATTGTATAGTT381380.33988503369574485No Hit
TCCTGTCTGAGCGTCGCT361470.3221412846242616No Hit
TGTAAACATCCTACACTCTCAGCT338830.30196456543900896No Hit
CTCCCACTGCTTCACTTGACTAG337680.30093968791855663No Hit
ATACCGGGTGCTGTAGGCTTA317550.2829998753214216No Hit
CTTCTCACTACTGCACTTGACTAG316620.28217106132662106No Hit
CCCCCCACTGCTAAATTTGACTGGCT311770.2778487517838439No Hit
CAAAGAATTCTCCTTTTGGGCT311530.27763486430131473No Hit
AGCTCGGTCTGAGGCCCCTCAGT308010.2744978478908867No Hit
TTCTCACTACTGCACTTGACTAG287870.256549123315313No Hit
CGCCTGTCTGAGCGTCGCT285410.25435677661938894No Hit
CAACACCAGTCGATGGGCTGT285330.2542854807918792No Hit
CCCCCACTGCTAAATTTGACTGGCTT280690.25015032279631505No Hit
AACATTCAACGCTGTCGGTGAGTT270460.24103336885350873No Hit
CACCCGGCTGTGTGCACATGTGC270450.24102445687507No Hit
ATACCGGGTGCTGTAGGCTTT260860.23247786955234154No Hit
CTACGCCTGTCTGAGCGTCGCT248470.2214359282667726No Hit
ATCCCGGACGAGCCCCCA248090.2210972730861014No Hit
TCGTTTCCCGGGCGGCGCACCA232760.20743521013955No Hit
CCCCCCACTGCTAAATTTGACTGG231060.2059201738049683No Hit
TTCAAGTAATCCAGGATAGGCTT229530.20455664110384475No Hit
AACGGAATCCCAAAAGCAGCTG227830.20304160476926308No Hit
CAAAGAATTCTCCTTTTGGGCTT214600.19125105729484204No Hit
ATACCGGGTGCTGTAGGCTT213130.1899409964643508No Hit
TATTGCACTTGTCCCGGCCTGTAG205930.18352437198847538No Hit
GGAATACCGGGTGCTGTAGGCTTT201410.17949615773417582No Hit
AACTTAACTTGACCGCTCTGACCA185900.16567367917572753No Hit
TAGCAGCACGTAAATATTGGC184260.16421211471177816No Hit
TATTGCACTTGTCCCGGCCTGTAAT182990.16308029345006123No Hit
TCGTTTCCCGGCCAATGCACCA181990.16218909560618966No Hit
TATTGCACTTGTCCCGGCCTGTT177070.15780440221434144No Hit
TACGCCTGTCTGAGCGTCGCT171710.15302758177118977No Hit
CACTAGATTGTGAGCTCCTGGAA166190.1481081696730186No Hit
TTCAAGTAATCCAGGATAGGC165760.14772495460015383No Hit
CCCATAAAGTAGAAAGCACTA163200.14544348811984256No Hit
CACTAGATTGTGAGCTCCTGGA162490.14481073765069374No Hit
CCCCCCACTGCTAAATTTGACTGT161420.14385715595775114No Hit
TGTCAGTTTGTCAAATACCCCAA156740.13968635004843213No Hit
TAGCTTATCAGACTGATGTTGACA155140.13826043349823763No Hit
ACCCCCCACTGCTAAATTTGACTG153940.1371909960855917No Hit
AGCAGCATTGTACAGGGCTATGT151450.13497191345435147No Hit
TATTGCACTTGTCCCGGCCTGTTT145340.12952669462829608No Hit
TTCACAGTGGCTAAGTTCCGC144010.1283414014959469No Hit
CCCCCCACTGCTAAATTTGACTGA141650.12623817458440992No Hit
TTCACAGTGGCTAAGTTCCG141030.12568563192120955No Hit
ACTGGACTTGGAGTCAGAAGGCT139810.12459837055168621No Hit
ACCACAGGGTAGAACCACGGAC137830.12283379882082049No Hit
CAACGGAATCCCAAAAGCAGCTGA136650.121782185365052No Hit
TTCACAGTGGCTAAGTTCTGC130720.11649738215089353No Hit
CCCCCCACTGCTAAATTTGACTGTA127810.11390399642522721No Hit
TGTAAACATCCCCGACTGGAAGC127580.11369902092113675No Hit
ACGCCTGTCTGAGCGTCGCT126190.11246025591815523No Hit
GGAATACCGGGTGCTGTAGGCTT126130.11240678404752294No Hit
TCAGTGCACTACAGAACTTTGT125770.11208595282372917No Hit
TGAGGTAGTAGTTTGTACAGTT125730.11205030490997431No Hit
ACTGGACTTGGAGTCAGAAGGCA122570.1092341197233401No Hit
ATCCCACCGCTGCCACCA121920.10865484112482357No Hit
ATCACATTGCCAGGGATTTCC121490.1082716260519588No Hit
CAAAGTGCTGTTCGTGCAGGTAG118250.10538414503781486No Hit
ACTTGACTTGGAGTCAGAAGGC117090.10435035553892381No Hit
CAAAGTGCTGTTCGTGCAGGT116940.10421667586234308No Hit
TATTGCACTTGTCCCGGCCTGTAAA116110.10347698165192967No Hit
TGAGGTAGTAGTTTGTGCTGTT116020.10339677384598121No Hit
TGTCAGTTTGTCAAATACCCCA113690.10132028286976044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)