FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795910
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5294759.135321286907491No Hit
CCCCCCACTGCTAAATTTGACTG3820556.59180353042059No Hit
AAGCTGCCAGTTGAAGAACTGT2288753.9489053487718064No Hit
AACATTCAACGCTGTCGGTGAGT2170243.7444335747104422No Hit
TAGCAGCACGTAAATATTGGCG1843353.1804324083707307No Hit
TTCAAGTAATCCAGGATAGGCT1783173.0766005683318065No Hit
AACATTCAACGCTGTCGGTGAG1159302.0002035918432135No Hit
CCCCCACTGCTAAATTTGACTG1041221.7964737202613565No Hit
TAGCTTATCAGACTGATGTTGAC938221.6187621961003535No Hit
TATTGCACTTGTCCCGGCCTGTAA903261.5584437991618227No Hit
TATTGCACTTGTCCCGGCCTGTAT768061.3251758567679623No Hit
TAGCTTATCAGACTGATGTTGACT757261.3065420270501096No Hit
TGTAAACATCCTTGACTGGAAGCT695241.1995355345407366No Hit
TATTGCACTTGTCCCGGCCTGT490110.8456135447237794No Hit
AGCAGCATTGTACAGGGCTATGA463640.7999434083690051No Hit
CGCCTGTCTGAGCGTCGCT415380.7166777952038593No Hit
CATAAAGTAGAAAGCACTACT411090.7092760239548233No Hit
TAGCTTATCAGACTGATGTTGA410800.7087756711198069No Hit
CAACGGAATCCCAAAAGCAGCTGT405260.6992172066163899No Hit
TGAGGTAGTAGGTTGTATAGTT394280.6802728130699062No Hit
TGTAAACATCCCCGACTGGAAGCT378010.6522012936708818No Hit
CCCATAAAGTAGAAAGCACTAC374140.6455241713553178No Hit
TATTGCACTTGTCCCGGCCTGTA345230.5956441697679915No Hit
TGTAAACATCCTACACTCTCAGCT291890.503613755217041No Hit
ACTGGACTTGGAGTCAGAAGGC282890.488085563785497No Hit
TTAATGCTAATCGTGATAGGGGTT279280.48185703366684435No Hit
TTCAAGTAATTCAGGATAGGTT267730.461929187996363No Hit
TGAGGTAGTAGATTGTATAGTT263770.45509678376648366No Hit
TGTCAGTTTGTCAAATACCCCAA239700.41356749846012103No Hit
CCCCCACTGCTAAATTTGACTGGCTT206170.3557163586046022No Hit
GCCTGTCTGAGCGTCGCT205750.35499170967113014No Hit
CCCATAAAGTAGAAAGCACT203290.35074733734650815No Hit
AACATTCAACGCTGTCGGTGAGTT188190.3246944828335844No Hit
TCGTACGACTCTTAGCGG178890.3086486850209889No Hit
CAAAGAATTCTCCTTTTGGGCT176850.305128961629839No Hit
CATTGCACTTGTCTCGGTCTGA158490.2734514511094893No Hit
TTCTCACTACTGCACTTGACTAG152730.26351340859330113No Hit
TAGCTTATCAGACTGATGTTGACA151890.26206411072635705No Hit
TGTCAGTTTGTCAAATACCCCA149370.2577162171255247No Hit
TACGCCTGTCTGAGCGTCGCT144670.2496070504890518No Hit
CCCCCCACTGCTAAATTTGACTGGCT136770.23597674912136318No Hit
CTACGCCTGTCTGAGCGTCGCT133860.23095596722516396No Hit
CTTCTCACTACTGCACTTGACTAG126600.21842989280371847No Hit
CAAAGAATTCTCCTTTTGGGCTT122640.21159748857383914No Hit
AGCTCGGTCTGAGGCCCCTCAGT122000.21049326162759602No Hit
AACGGAATCCCAAAAGCAGCTG121340.20935452758928277No Hit
AACCGGGCGGAAACACCA118600.2046270559756794No Hit
TGAGGTAGTAGTTTGTACAGTT117700.20307423683252498No Hit
ACGCCTGTCTGAGCGTCGCT114870.19819148330460618No Hit
CAACGGAATCCCAAAAGCAGCT114220.19707000281232798No Hit
TAGCAGCACGTAAATATTGGC114170.19698373508215275No Hit
GCGTTCGCGCTTTCCCCTG105290.18166258620302939No Hit
CCCCCCACTGCTAAATTTGACTGG104420.1801615276979801No Hit
TGTAAACATCCCCGACTGGAAGC103610.17876399046914118No Hit
TGTAAACATCCTACACTCAGCT102860.17746997451651253No Hit
GGAATACCGGGTGCTGTAGGCTTT102750.17728018551012695No Hit
ATCACATTGCCAGGGATTTCCA100830.1739675046713976No Hit
TATTGCACTTGTCCCGGCCTGTAG98740.1703615135500724No Hit
TTCAAGTAATCCAGGATAGGCTT98380.16974038589281062No Hit
CAACACCAGTCGATGGGCTGT93730.1617174869865129No Hit
TGAGGTAGTAGTTTGTGCTGTT93630.1615449515261624No Hit
TAGCAGCACGTAAATATTGGCGT93600.16149319088805725No Hit
CTCCCACTGCTTCACTTGACTAG91430.15774917139845165No Hit
AGCAGCATTGTACAGGGCTATGT87550.1510547955368527No Hit
TATTGCACTTGTCCCGGCCTGTT87120.1503128930573456No Hit
ACTGGACTTGGAGTCAGAAGGCA86820.14979528667629416No Hit
TATTGCACTTGTCCCGGCCTGTAAT86400.1490706377428221No Hit
TGAGAACTGAATTCCATGGGTT84060.14503330797062064No Hit
ATCACATTGCCAGGGATTTCC81010.13977097642993078No Hit
ATCCCGGACGAGCCCCCA78960.13623399949274576No Hit
CACCCGGCTGTGTGCACATGTGC77840.13430160233682029No Hit
TAGCAGCACATCATGGTTTACA77820.13426709524475017No Hit
TTCACAGTGGCTAAGTTCCG74890.12921180625648088No Hit
ACCCCCCACTGCTAAATTTGACTG74760.12898751015802523No Hit
ACCACAGGGTAGAACCACGGAC74580.12867694632939436No Hit
ATCCCACCGCTGCCACCA73950.12758997292918625No Hit
TATTGCACTTGTCCCGGCCTGTAAA73830.12738293037676568No Hit
TTCAAGTAATCCAGGATAGGC70940.12239665557263657No Hit
TTCACAGTGGCTAAGTTCCGC70840.12222412011228607No Hit
AACATTCAACGCTGTCGGTGAGA70520.12167200663916451No Hit
TCGTTTCCCGGCCAATGCACCA69890.12058503323895643No Hit
CAAAGTGCTGTTCGTGCAGGTAG68230.11772094459713833No Hit
GGAATACCGGGTGCTGTAGGCTT67890.11713432403194667No Hit
TGAGAACTGAATTCCATAGGCTGT67570.1165822105588251No Hit
TTCACAGTGGCTAAGTTCTGC67010.11561601198086235No Hit
AACATTCAACGCTGTCGGTGA66240.11428748893616361No Hit
ACTGGACTTGGAGTCAGAAGGCT65820.11356284000269155No Hit
AGCAGCATTGTACAGGGCTATG64960.11207903504367736No Hit
TGAGGTAGTAGGTTGTGTGGTT64770.11175121766901143No Hit
TTATAAAGCAATGAGACTGATT64610.11147516093245063No Hit
CCAAGTCCTTCTGATCGAGGCCC63240.10911142512564896No Hit
CTCCCACTGCTTCACTTGACTAGCCT61300.10576423719484947No Hit
ATGCACCTGGGCAAGGATTCTGA60700.10472902443274654No Hit
TATTGCACTTGTCCCGGCCTGTTT60340.10410789677548478No Hit
CCCATAAAGTAGAAAGCACTA59680.10296916273717156No Hit
TGTCAGTTTGTCAAATACCCC59230.10219275316559435No Hit
CCCCCACTGCTAAATTTGACTGGCTA59210.10215824607352425No Hit
CAAAGTGCTGTTCGTGCAGGT57980.10003605991121325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)