FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5545336
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG127762223.039577764088595No Hit
CCCCCCACTGCTAAATTTGACTG3647416.577437327512706No Hit
TTCAAGTAATCCAGGATAGGCT1302772.349307598313249No Hit
CCCCCACTGCTAAATTTGACTG1133022.0431944971413816No Hit
TCGTACGACTCTTAGCGG1100741.9849834166946783No Hit
TAGCTTATCAGACTGATGTTGAC1014981.830330930353003No Hit
TGAGAACTGAATTCCATGGGTT949601.712430049324333No Hit
TATTGCACTTGTCCCGGCCTGTAA942691.699969127208883No Hit
TAGCAGCACGTAAATATTGGCG891831.6082524124778013No Hit
AACATTCAACGCTGTCGGTGAG885041.5960078884309263No Hit
TATTGCACTTGTCCCGGCCTGTAT789051.4229074667432235No Hit
TGAGAACTGAATTCCATAGGCTGT745281.343976271230454No Hit
TGAGGTAGTAGATTGTATAGTT657291.185302387447758No Hit
AAGCTGCCAGTTGAAGAACTGT566311.0212365851230656No Hit
TAGCTTATCAGACTGATGTTGACT557911.0060887203228084No Hit
CAACGGAATCCCAAAAGCAGCTGT525740.9480760047722987No Hit
TTAATGCTAATCGTGATAGGGGTT521780.9409348685093202No Hit
TATTGCACTTGTCCCGGCCTGT505890.9122801575955001No Hit
TGAGGTAGTAGGTTGTATAGTT476280.858883934174593No Hit
CAACGGAATCCCAAAAGCAGCT450730.8128091787404766No Hit
TGTAAACATCCTTGACTGGAAGCT377820.681329318908719No Hit
TAGCTTATCAGACTGATGTTGA356010.6419989699451936No Hit
TATTGCACTTGTCCCGGCCTGTA355700.6414399416013745No Hit
TCGTTTCCCGGCCAATGCACCA348010.6275724320401865No Hit
CCCCCACTGCTAAATTTGACTGGCTT321720.5801632218498572No Hit
AGCAGCATTGTACAGGGCTATGA283470.5111863374915424No Hit
AACATTCAACGCTGTCGGTGAGT258500.46615750605553924No Hit
AACGGAATCCCAAAAGCAGCTG241420.4353568476283493No Hit
TAGCTTATCAGACTGATGTTGACA224180.4042676584430592No Hit
TTCAAGTAATTCAGGATAGGTT218320.39370021942764155No Hit
TGTAAACATCCCCGACTGGAAGCT214480.3867754812332382No Hit
CCCATAAAGTAGAAAGCACTAC204630.3690128064376983No Hit
CAAAGAATTCTCCTTTTGGGCT202690.3655143709957341No Hit
TGAGGTAGTAGTTTGTACAGTT201860.3640176176880896No Hit
CACCCGTAGAACCGACCTTGCG160950.2902439094763599No Hit
CACCCGGCTGTGTGCACATGTGC160400.28925208499539073No Hit
ACTGGACTTGGAGTCAGAAGGC156190.2816601194228808No Hit
TGAGAACTGAATTCCATGGGTTG152930.27578130522659045No Hit
CATTGCACTTGTCTCGGTCTGA143950.25958751642822003No Hit
TTAATGCTAATCGTGATAGGGGTTT137100.24723479334705778No Hit
CAAAGAATTCTCCTTTTGGGC133700.24110351473742977No Hit
AATGCACCCGGGCAAGGATTCT132960.23976905998121667No Hit
ATGCACCTGGGCAAGGATTCTGA131460.23706408412402782No Hit
CATAAAGTAGAAAGCACTACT128390.23152790020298136No Hit
TAGCAGCACGTAAATATTGGC124340.2242244653885716No Hit
TATTGCACTTGTCCCGGCCTGTAAA122660.22119489242852008No Hit
TGAGGTAGTAGTTTGTGCTGTT122020.22004076939611955No Hit
TTCACAGTGGCTAAGTTCCGC114420.20633555838636286No Hit
TTCTCACTACTGCACTTGACTAG111790.20159283405009185No Hit
TATTGCACTTGTCCCGGCCTGTAAT111540.20114200474056038No Hit
TCGTTTCCCGGCCAACGCACCA109290.19708454095477712No Hit
GCCTGTCTGAGCGTCGCT106850.19268444689375No Hit
AATCCTTGGAACCTAGGTGTGAGT104700.18880731483177934No Hit
GGAATACCGGGTGCTGTAGGCTTT103380.1864269360774532No Hit
TATTGCACTTGTCCCGGCCTGTAG102620.18505641497647754No Hit
TGTAAACATCCTACACTCTCAGCT100450.1811432165697444No Hit
CCCATAAAGTAGAAAGCACT97260.17539063458012283No Hit
TGAGAACTGAATTCCATGGGTTT96340.17373158272104702No Hit
CAAAGAATTCTCCTTTTGGGCTT95640.1724692606543589No Hit
CTTCTCACTACTGCACTTGACTAG94350.17014298141717654No Hit
ACCCCCCACTGCTAAATTTGACTG94230.16992658334860142No Hit
CCCCCCACTGCTAAATTTGACTGGCT88750.1600444048836716No Hit
CCCATAAAGTAGAAAGCACTA87380.15757386026743916No Hit
CTCCCACTGCTTCACTTGACTAGCCT85450.15409345799785623No Hit
AACCGGGCGGAAACACCA81500.14697035490725902No Hit
TATTGCACTTGTCCCGGCCTGTT79120.14267845988051941No Hit
ATCACATTGCCAGGGATTTCC78690.1419030334681253No Hit
AGCTCGGTCTGAGGCCCCTCAGT77980.14062267822905591No Hit
TGATAACTGAATTCCATGGGTT72780.13124542859080135No Hit
CACTAGATTGTGAGCTCCTGGAA72750.13119132907365758No Hit
CAACGGAATCCCAAAAGCAGCTGA68270.12311246784685365No Hit
GGAATACCGGGTGCTGTAGGCTT66090.11918123626773923No Hit
TGAGGTAGTAGTTTGTGCTGTTA65750.11856810840677644No Hit
NAACGGAATCCCAAAAGCAGCTG65150.11748611806390091No Hit
TGTCAGTTTGTCAAATACCCCA64480.11627789551435658No Hit
TAGCAGCACATCATGGTTTACA63550.11460081048289951No Hit
TCCTGTACTGAGCTGCCCCGAGA62130.11204010000476075No Hit
TGAGGTAGTAGATTGTATAGTTA60140.10845149870089026No Hit
ACTGGACTTGGAGTCAGAAGGCA59540.10736950835801473No Hit
CCCCCCACTGCTAAATTTGACTGG58930.10626948484275796No Hit
TCGTTTCCCGGGCGGCGCACCA56690.10223005422935598No Hit
CAAAGTGCTGTTCGTGCAGGTAG56520.10192349029887458No Hit
TTCACAGTGGCTAAGTTCTGC56510.10190545712649333No Hit
ACTGGACTTGGAGTCAGAAGGCT56380.10167102588553695No Hit
TGATAACTGAATTCCATAGGCTGT55720.10048083650837389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)