FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10610675
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8196407.724673500978967No Hit
CCCCCCACTGCTAAATTTGACTG5502875.186163933962731No Hit
AACATTCAACGCTGTCGGTGAGT4396024.143016349101259No Hit
AAGCTGCCAGTTGAAGAACTGT3641263.4316949675680384No Hit
TTCAAGTAATCCAGGATAGGCT3382043.1873938274426465No Hit
TAGCAGCACGTAAATATTGGCG2825542.662922010145443No Hit
AACATTCAACGCTGTCGGTGAG2515692.370904772787782No Hit
GCCTGTCTGAGCGTCGCT2391482.2538434171247355No Hit
CCCCCACTGCTAAATTTGACTG2028941.9121686414860506No Hit
CGCCTGTCTGAGCGTCGCT1957701.84502870929512No Hit
TATTGCACTTGTCCCGGCCTGTAA1293991.2195171372226554No Hit
TATTGCACTTGTCCCGGCCTGT1255601.1833365926295925No Hit
TCGTACGACTCTTAGCGG1149561.0833995009742547No Hit
CATAAAGTAGAAAGCACTACT1141671.0759635932681004No Hit
AACCGGGCGGAAACACCA1125291.0605263095891637No Hit
TAGCTTATCAGACTGATGTTGAC1103491.039980962568357No Hit
TATTGCACTTGTCCCGGCCTGTAT1098411.035193331244242No Hit
TCCTGTCTGAGCGTCGCT1005330.9474703541480632No Hit
TGAGGTAGTAGGTTGTATAGTT978920.9225803259453333No Hit
TAGCTTATCAGACTGATGTTGACT976440.9202430571099387No Hit
TAGCTTATCAGACTGATGTTGA843060.7945394614385984No Hit
TATTGCACTTGTCCCGGCCTGTA799190.7531943066770022No Hit
TGTAAACATCCTTGACTGGAAGCT791890.7463144427663649No Hit
TTAATGCTAATCGTGATAGGGGTT748840.7057420946358267No Hit
AGCAGCATTGTACAGGGCTATGA687960.6483659145153348No Hit
CCCATAAAGTAGAAAGCACTAC583900.5502948681398685No Hit
CCCATAAAGTAGAAAGCACT560010.5277798066569752No Hit
ACTGGACTTGGAGTCAGAAGGC553220.5213805907729715No Hit
CAACGGAATCCCAAAAGCAGCTGT548330.5167720243999557No Hit
TACGCCTGTCTGAGCGTCGCT529270.4988089824634154No Hit
TGAGGTAGTAGATTGTATAGTT517430.4876504086686285No Hit
TGTAAACATCCCCGACTGGAAGCT481920.45418411175537843No Hit
ATCCCGGACGAGCCCCCA478180.4506593595600657No Hit
TTCAAGTAATTCAGGATAGGTT445330.41969997196219844No Hit
CAACACCAGTCGATGGGCTGT415900.39196375348410917No Hit
ACGCCTGTCTGAGCGTCGCT399260.3762814335563006No Hit
CATTGCACTTGTCTCGGTCTGA380460.3585634278686323No Hit
CTACGCCTGTCTGAGCGTCGCT379210.35738536897982454No Hit
AACATTCAACGCTGTCGGTGAGTT378160.3563957995132261No Hit
TTCACAGTGGCTAAGTTCCG297140.280038734576264No Hit
TGTAAACATCCTACACTCTCAGCT294640.27768261679864853No Hit
AACGGAATCCCAAAAGCAGCTG256880.2420958138855445No Hit
GCGTTCGCGCTTTCCCCTG247760.23350069623280328No Hit
TGAGGTAGTAGTTTGTACAGTT247440.2331991131572685No Hit
CAACGGAATCCCAAAAGCAGCT238510.22478306045562607No Hit
ACCGGGTGCTGTAGGCTT236020.22243636714912104No Hit
TGTCAGTTTGTCAAATACCCCAA234150.22067399105146468No Hit
ATCCCACCGCTGCCACCA228070.21494391261630388No Hit
AGCTCGGTCTGAGGCCCCTCAGT220600.20790383269678886No Hit
TGAGGTAGTAGTTTGTGCTGTT210510.19839454134633283No Hit
TATTGCACTTGTCCCGGCCTGTT208650.1966415897197869No Hit
TGAGGTAGTAGGTTGTGTGGTT192580.1814964646452747No Hit
TTCTCACTACTGCACTTGACTAG188190.17735912182778193No Hit
ACCACAGGGTAGAACCACGGAC181010.1705923515704703No Hit
TCGTTTCCCGGCCAATGCACCA166510.1569268684603006No Hit
ACTGGACTTGGAGTCAGAAGG166430.15685147269141692No Hit
TTCAAGTAATCCAGGATAGGC165160.15565456486038826No Hit
ACCGGGTGCTGTAGGCTTT164550.1550796721226501No Hit
ACTGGACTTGGAGTCAGAAGGCA156010.14703117379431563No Hit
TTCACAGTGGCTAAGTTCTG152690.14390224938564228No Hit
TAGCAGCACGTAAATATTGGC151660.14293152886126473No Hit
AGCAGCATTGTACAGGGCTATG151020.14232836271019517No Hit
TTATAAAGCAATGAGACTGATT150950.14226239141242192No Hit
TATTGCACTTGTCCCGGCCTGTAG147500.1390109488793126No Hit
TTCAAGTAATCCAGGATAGGCTT145480.13710720571499926No Hit
CAAAGAATTCTCCTTTTGGGCT144450.13613648519062171No Hit
ACTGGACTTGGAGTCAGAAGGCT140480.13239497015976834No Hit
AGCAGCATTGTACAGGGCTATGT140470.13238554568865787No Hit
CCCATAAAGTAGAAAGCACTA137680.12975611824883904No Hit
TGAGGTAGTAGGTTGTATAGT137220.12932259257775777No Hit
TGTCAGTTTGTCAAATACCCCA132920.12527007000025916No Hit
AACATTCAACGCTGTCGGTGA132260.1246480549069687No Hit
TGAGAACTGAATTCCATGGGTT130840.1233097800092831No Hit
AACATTCAACGCTGTCGGTGAGA130060.12257467126266708No Hit
CCTCGACACAAGGGTTTGT129620.12215999453380674No Hit
CAAAGTGCTGTTCGTGCAGGT129430.12198092958270797No Hit
CTTCTCACTACTGCACTTGACTAG128610.12120812295165011No Hit
ACCGGGTGCTGTAGGCTTA124090.11694826200972135No Hit
TAGCTTATCAGACTGATGTTGACA122680.11561941158314622No Hit
TGTAAACATCCTACACTCAGCT121350.11436595692545477No Hit
TACCTGGTTGATCCTGCC120060.11315020015220521No Hit
CCCCCCACTGCTAAATTTGACTGG118260.11145379535232207No Hit
TCGTTTCCCGGGCGGCGCACCA117960.11117106121900822No Hit
CCCCCACTGCTAAATTTGACTGGCTT116520.10981393737910172No Hit
CACCCGGCTGTGTGCACATGTGC114630.10803271233922442No Hit
CCCCCCACTGCTAAATTTGACTGGCT113630.10709026522817823No Hit
AATGACACGATCACTCCCGTTG112520.10604414893491695No Hit
AGAGGTAGTAGGTTGCATAGTT111520.10510170182387077No Hit
TGTAAACATCCCCGACTGGAAGC110780.1044042909616966No Hit
TATTGCACTTGTCCCGGCCTGA110500.10414040577060367No Hit
TATTGCACTTGTCCCGGCCTG109570.1032639299573307No Hit
TCGTTCGCGCTTTCCCCTG108180.1019539284729765No Hit
AGCTACATTGTCTGCTGGGTTT107780.10157694962855802No Hit
TTCACAGTGGCTAAGTTCTGC106940.10078529405527924No Hit
TAGCAGCACGTAAATATTGGCGT106330.10021040131754105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)