FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9610581
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6021396.26537563129638No Hit
CCCCCCACTGCTAAATTTGACTG5743275.975986259311481No Hit
AACATTCAACGCTGTCGGTGAGT3384553.521691352479106No Hit
AAGCTGCCAGTTGAAGAACTGT3051163.174792450113058No Hit
TAGCAGCACGTAAATATTGGCG2950063.0695958964395595No Hit
TTCAAGTAATCCAGGATAGGCT2625592.7319784308565733No Hit
CGCCTGTCTGAGCGTCGCT1981892.0621958235407414No Hit
AACATTCAACGCTGTCGGTGAG1952452.0315629200773606No Hit
CCCCCACTGCTAAATTTGACTG1908001.9853118141348582No Hit
TAGCTTATCAGACTGATGTTGAC1620701.6863704702140276No Hit
TATTGCACTTGTCCCGGCCTGTAA1349141.4038069082399909No Hit
TAGCTTATCAGACTGATGTTGACT1161261.2083140446971936No Hit
TATTGCACTTGTCCCGGCCTGTAT1104211.1489523890387063No Hit
TGTAAACATCCTTGACTGGAAGCT1068621.1119202886901427No Hit
TGAGGTAGTAGGTTGTATAGTT934920.9728027889260806No Hit
TATTGCACTTGTCCCGGCCTGT923320.9607327590288246No Hit
TAGCTTATCAGACTGATGTTGA835950.8698225424664752No Hit
AGCAGCATTGTACAGGGCTATGA811720.8446107472586725No Hit
CATAAAGTAGAAAGCACTACT708060.7367504628492284No Hit
TGAGGTAGTAGATTGTATAGTT664800.6917375754910136No Hit
TATTGCACTTGTCCCGGCCTGTA655130.6816757488439044No Hit
GCCTGTCTGAGCGTCGCT640900.6668691518233913No Hit
TACGCCTGTCTGAGCGTCGCT609470.6341656139207401No Hit
ACTGGACTTGGAGTCAGAAGGC550360.5726604874356712No Hit
GCGTTCGCGCTTTCCCCTG535630.5573336305057935No Hit
TGTAAACATCCCCGACTGGAAGCT535610.557312820109419No Hit
TTCAAGTAATTCAGGATAGGTT498500.5186991296363872No Hit
TCGTACGACTCTTAGCGG469680.48871134846061853No Hit
CTACGCCTGTCTGAGCGTCGCT418520.43547835453444494No Hit
ACGCCTGTCTGAGCGTCGCT411950.4286421393253957No Hit
CAACGGAATCCCAAAAGCAGCTGT374270.3894353525556884No Hit
CCCATAAAGTAGAAAGCACTAC373870.3890191446281968No Hit
TGTCAGTTTGTCAAATACCCCAA371580.38663635424330745No Hit
AACATTCAACGCTGTCGGTGAGTT354910.36929088886509565No Hit
AACCGGGCGGAAACACCA347380.36145577463006656No Hit
CATTGCACTTGTCTCGGTCTGA340060.3538391695569706No Hit
CCCATAAAGTAGAAAGCACT317620.3304899048246927No Hit
TGTAAACATCCTACACTCTCAGCT283480.29496655821328593No Hit
TGAGGTAGTAGTTTGTACAGTT269850.2807842730840102No Hit
CAACGGAATCCCAAAAGCAGCT257590.26802750010639315No Hit
CAACACCAGTCGATGGGCTGT243150.2530023939239469No Hit
TTCTCACTACTGCACTTGACTAG239600.24930854856745913No Hit
TTCACAGTGGCTAAGTTCCG227980.2372177082738286No Hit
CCTCGACACAAGGGTTTGT222110.23110985693788963No Hit
TGAGGTAGTAGTTTGTGCTGTT214280.22296258675724182No Hit
CCAAGTCCTTCTGATCGAGGCCC211210.21976819091374394No Hit
CCCCCACTGCTAAATTTGACTGGCTT210160.21867564510407853No Hit
TGTCAGTTTGTCAAATACCCCA197520.20552347459534442No Hit
TTATAAAGCAATGAGACTGATT193180.20100761858206076No Hit
CTTCTCACTACTGCACTTGACTAG189740.19742823040563312No Hit
TAGCTTATCAGACTGATGTTGACA186470.19402573059838943No Hit
TCCTGTCTGAGCGTCGCT186360.19391127341832923No Hit
AACGGAATCCCAAAAGCAGCTG174340.18140422519720711No Hit
TCGTTCGCGCTTTCCCCTG173320.18034289498210357No Hit
TCGTTTCCCGGCCAATGCACCA171500.17844914891201685No Hit
GGAATACCGGGTGCTGTAGGCTTT167960.17476570875371633No Hit
TAGCAGCACGTAAATATTGGC167280.17405815527698065No Hit
TTCAAGTAATCCAGGATAGGCTT163090.1696983772365063No Hit
CAAGTCCTTCTGATCGAGGCCC162460.16904284975070705No Hit
CTGCGTTCGCGCTTTCCCCTG160180.16667046456400503No Hit
TATTGCACTTGTCCCGGCCTGTT159030.16547386677246673No Hit
ACCACAGGGTAGAACCACGGAC158570.1649952276558514No Hit
ACTGGACTTGGAGTCAGAAGGCA156970.16333039594588508No Hit
AGCAGCATTGTACAGGGCTATGT156590.16293499841476808No Hit
ACTGGACTTGGAGTCAGAAGG155190.1614782706685475No Hit
CTCCCACTGCTTCACTTGACTAG154970.16124935630842716No Hit
TAGCAGCACGTAAATATTGGCGT154030.1602712676788219No Hit
CCCCCCACTGCTAAATTTGACTGGCT148180.1541842267392575No Hit
CTCGACACAAGGGTTTGT147980.1539761227755117No Hit
CAAAGAATTCTCCTTTTGGGCT146870.15282114577672257No Hit
TGTAAACATCCCCGACTGGAAGC144420.1502718722208366No Hit
TATTGCACTTGTCCCGGCCTGTAG143160.1489608172492381No Hit
AGCAGCATTGTACAGGGCTATG140230.14591209418036225No Hit
ATCCCGGACGAGCCCCCA139420.1450692731271918No Hit
AGCTCGGTCTGAGGCCCCTCAGT139400.1450484627308172No Hit
CAAAGAATTCTCCTTTTGGGCTT138280.14388308053384077No Hit
TACCTGGTTGATCCTGCC137390.142957017895172No Hit
TGTAAACATCCTACACTCAGCT136990.1425408099676804No Hit
ACCGGGTGCTGTAGGCTT130700.13599594030787523No Hit
TTCACAGTGGCTAAGTTCTG129520.13476812692177506No Hit
ATCCCACCGCTGCCACCA128980.13420624621966143No Hit
CCCCCCACTGCTAAATTTGACTGG127090.1322396637622637No Hit
GGAATACCGGGTGCTGTAGGCTT124190.1292221562879497No Hit
CAAAGTGCTGTTCGTGCAGGT119400.12423806635623799No Hit
TGAGGTAGTAGGTTGTGTGGTT119210.12404036759067949No Hit
TTCAAGTAATCCAGGATAGGC119090.12391550521243201No Hit
TGAGGTAGTAGGTTGTATAGT117280.12203216434053259No Hit
CAAAGTGCTGTTCGTGCAGGTAG116280.12099164452180361No Hit
GTCAGGATGGCCGAGCGGTCTAAGG113510.11810940462392439No Hit
TTCACAGTGGCTAAGTTCTGC111990.11652781449945637No Hit
ACTGGACTTGGAGTCAGAAGGCT111440.11595552859915545No Hit
CACCCGGCTGTGTGCACATGTGC105200.10946268493028674No Hit
AACATTCAACGCTGTCGGTGA103890.10809960396775178No Hit
AACATTCAACGCTGTCGGTGAGA103310.10749610247288899No Hit
AATGACACGATCACTCCCGTTG100550.10462426777319705No Hit
CAACGGAATCCCAAAAGCAGCTGA99720.10376063632365203No Hit
TTCACAGTGGCTAAGTTCCGC99440.10346929077440792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)