FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388897
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4833366.541382292918686No Hit
AACATTCAACGCTGTCGGTGAGT3185884.311712560074934No Hit
AAGCTGCCAGTTGAAGAACTGT2724923.6878576058104477No Hit
TTCAAGTAATCCAGGATAGGCT2305753.120560484196762No Hit
TAGCAGCACGTAAATATTGGCG1858632.5154363364383077No Hit
GCCTGTCTGAGCGTCGCT1579162.137206676449814No Hit
CGCCTGTCTGAGCGTCGCT1562042.1140367770724104No Hit
CCCCCCACTGCTAAATTTGACTG1542952.0882007152082376No Hit
AACATTCAACGCTGTCGGTGAG1525592.0647060041573186No Hit
TGAGGTAGTAGGTTGTATAGTT1175801.5913065238289288No Hit
TAGCTTATCAGACTGATGTTGAC937371.268619660011501No Hit
TATTGCACTTGTCCCGGCCTGT861441.1658573668031913No Hit
TATTGCACTTGTCCCGGCCTGTAA798591.0807973098014494No Hit
TAGCTTATCAGACTGATGTTGA771061.0435387040853323No Hit
TAGCTTATCAGACTGATGTTGACT724890.9810530583928831No Hit
TCGTACGACTCTTAGCGG697110.9434561071835215No Hit
TATTGCACTTGTCCCGGCCTGTAT678620.9184320745031362No Hit
TCCTGTCTGAGCGTCGCT642580.8696561881969664No Hit
CCCCCACTGCTAAATTTGACTG599350.8111494855050762No Hit
CATAAAGTAGAAAGCACTACT593120.8027179158134158No Hit
TGAGGTAGTAGATTGTATAGTT543290.7352788920998627No Hit
ACTGGACTTGGAGTCAGAAGGC479190.6485271076318968No Hit
AGCAGCATTGTACAGGGCTATGA469540.6354669715926478No Hit
TGTAAACATCCTTGACTGGAAGCT452410.6122835383955142No Hit
TATTGCACTTGTCCCGGCCTGTA423340.5729407244410093No Hit
ACCGGGTGCTGTAGGCTT380760.5153137200315554No Hit
TACGCCTGTCTGAGCGTCGCT357560.4839152582584383No Hit
TTCAAGTAATTCAGGATAGGTT340740.4611513734729284No Hit
ACGCCTGTCTGAGCGTCGCT317920.43026719684954334No Hit
CAACGGAATCCCAAAAGCAGCTGT309200.41846570604516475No Hit
TGTAAACATCCCCGACTGGAAGCT282780.3827093543190547No Hit
CTACGCCTGTCTGAGCGTCGCT280560.37970484633904084No Hit
AACCGGGCGGAAACACCA276270.3738988376749601No Hit
CCCATAAAGTAGAAAGCACTAC275420.37274846299792785No Hit
CAACACCAGTCGATGGGCTGT268400.3632477215476139No Hit
TGAGGTAGTAGTTTGTACAGTT259870.3517033733181015No Hit
CACCCGGCTGTGTGCACATGTGC254910.3449905987321247No Hit
CCCATAAAGTAGAAAGCACT249180.33723572002695396No Hit
CATTGCACTTGTCTCGGTCTGA242690.32845227102231905No Hit
AACATTCAACGCTGTCGGTGAGTT236700.3203455130041737No Hit
CGCGACCTCAGATCAGACGTGG223070.3018989167124674No Hit
ACCGGGTGCTGTAGGCTTT219460.2970132077900125No Hit
ATCCCGGACGAGCCCCCA218160.29525381122513955No Hit
ACCGGGTGCTGTAGGCTTA205660.2783365365628997No Hit
GCGTTCGCGCTTTCCCCTG201170.27225985150422316No Hit
TGAGGTAGTAGGTTGTGTGGTT183830.24879220809276406No Hit
CGCGACCTCAGATCAGACGTGGCG183330.24811551710627444No Hit
CTCCCACTGCTTCACTTGACTAG182090.24643732345978028No Hit
TTCTCACTACTGCACTTGACTAG179360.2427425906735471No Hit
AGCTCGGTCTGAGGCCCCTCAGT163080.22070953215344594No Hit
TGAGGTAGTAGTTTGTGCTGTT162530.21996517206830735No Hit
TGTAAACATCCTACACTCTCAGCT156840.21226442864205577No Hit
AACGGAATCCCAAAAGCAGCTG152910.20694563748824757No Hit
ATCCCACCGCTGCCACCA149980.20298022830741855No Hit
CAACGGAATCCCAAAAGCAGCT124690.1687531982107749No Hit
ACCACAGGGTAGAACCACGGAC124180.1680629734045555No Hit
TATTGCACTTGTCCCGGCCTGTT123340.166926132547253No Hit
TGAGGTAGTAGGTTGTATAGT121630.16461184937345857No Hit
CTTCTCACTACTGCACTTGACTAG118280.1600780197639783No Hit
CCCCCACTGCTAAATTTGACTGGCTT114520.15498930354557655No Hit
TTCAAGTAATCCAGGATAGGCTT113700.15387953032773363No Hit
TTCAAGTAATCCAGGATAGGC110890.1500765269836621No Hit
ACTGGACTTGGAGTCAGAAGGCA108250.14650359857499706No Hit
CACTAGATTGTGAGCTCCTGGA106430.14404044338417493No Hit
CAAAGTGCTGTTCGTGCAGGT105390.14263292613227657No Hit
ACTGGACTTGGAGTCAGAAGG104520.14145548381578468No Hit
CCCCCCACTGCTAAATTTGACTGGCT103160.139614884332533No Hit
TTCACAGTGGCTAAGTTCCG103140.1395878166930734No Hit
CGCGACCTCAGATCAGACGTG103040.1394524784957755No Hit
TTATAAAGCAATGAGACTGATT101450.13730060115873857No Hit
AGAGGTAGTAGGTTGCATAGTT101440.13728706733900878No Hit
CGCGACCTCAGATCAGACGTGGC99870.13516225764143144No Hit
TAGCTTATCAGACTGATGTTGACA98440.13322692142007123No Hit
AGCAGCATTGTACAGGGCTATGT96820.13103444262384495No Hit
CGCGACCTCAGATCAGACGT95900.1297893312087041No Hit
TCGTTCGCGCTTTCCCCTG95510.1292615122392422No Hit
ACTGGACTTGGAGTCAGAAGGCT93760.12689309378652863No Hit
TACCACCCTGAACGCGCCCGA92230.12482241936787046No Hit
TACCTGGTTGATCCTGCC91760.12418632984057025No Hit
TAGCAGCACGTAAATATTGGC91560.12391565344597442No Hit
CTCCCACTGCTTCACTTGACTAGCCT91360.12364497705137857No Hit
TTCACAGTGGCTAAGTTCCGC90300.12221039216002064No Hit
CCTCGACACAAGGGTTTGT89130.120626935251635No Hit
CTCGACACAAGGGTTTGT88650.11997731190460498No Hit
TATTGCACTTGTCCCGGCCTGTAG88190.11935475619703456No Hit
AACATTCAACGCTGTCGGTGAGA86800.11747355525459349No Hit
AACATTCAACGCTGTCGGTGA85240.11536227937674595No Hit
AGCAGCATTGTACAGGGCTATG83900.11354874753295384No Hit
AATGACACGATCACTCCCGTTG83800.11341340933565593No Hit
CCCTGTCTGAGCGTCGCT83490.11299386092403235No Hit
CTTGACTAGCTTGCTGTTT83230.11264198161105778No Hit
CCCCCCACTGCTAAATTTGACTGG81670.11053070573321025No Hit
CACTAGATTGTGAGCTCCTGGAA78820.10667356711021955No Hit
GTCCGGCCGTGTCGGCGG78770.1066058980115706No Hit
TGTAAACATCCCCGACTGGAAGC77560.10496830582426579No Hit
TTCACAGTGGCTAAGTTCTGC76840.10399387080372077No Hit
CAAAGTGCTGTTCGTGCAGGTAG76600.10366905913020577No Hit
TAGTGGGTTATCAGAACT76220.10315477398047368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)