FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4799494
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG65810413.711945467584707No Hit
TCGTACGACTCTTAGCGG3105256.469952874198821No Hit
TTCAAGTAATCCAGGATAGGCT1259742.6247350241504623No Hit
AACATTCAACGCTGTCGGTGAG1167672.432902301784313No Hit
GCCTGTCTGAGCGTCGCT1059092.2066701198084635No Hit
CCCCCCACTGCTAAATTTGACTG963722.007961672626323No Hit
TAGCAGCACGTAAATATTGGCG861971.7959601574665998No Hit
TGAGGTAGTAGATTGTATAGTT826011.7210355925020429No Hit
AAGCTGCCAGTTGAAGAACTGT777531.6200249442962111No Hit
TATTGCACTTGTCCCGGCCTGT636281.3257230866420502No Hit
TGAGAACTGAATTCCATGGGTT516691.0765509864164846No Hit
TATTGCACTTGTCCCGGCCTGTAA501001.0438600402459093No Hit
CCCCCACTGCTAAATTTGACTG481041.0022723228740362No Hit
TGAGGTAGTAGGTTGTATAGTT468200.9755195026809075No Hit
TAGCTTATCAGACTGATGTTGAC465760.9704356334230233No Hit
TATTGCACTTGTCCCGGCCTGTAT417420.8697166826336277No Hit
TAGCTTATCAGACTGATGTTGA399930.8332753411088751No Hit
GGAATACCGGGTGCTGTAGGCTT383950.7999801645756823No Hit
GGAATACCGGGTGCTGTAGGCTTT383940.7999593290459369No Hit
AACATTCAACGCTGTCGGTGAGT349660.7285351330786121No Hit
CACCCGGCTGTGTGCACATGTGC330960.6895726924546629No Hit
TTCAAGTAATTCAGGATAGGTT282710.5890412614329761No Hit
TAGCTTATCAGACTGATGTTGACT276070.5752064696820123No Hit
TATTGCACTTGTCCCGGCCTGTA274060.5710185282031814No Hit
TGAGGTAGTAGTTTGTACAGTT272290.5673306394382408No Hit
ACCGGGTGCTGTAGGCTT268780.5600173684975958No Hit
CAACGGAATCCCAAAAGCAGCTGT245640.511803952666677No Hit
CATTGCACTTGTCTCGGTCTGA214830.4476096855210153No Hit
CATAAAGTAGAAAGCACTACT214680.4472971525748339No Hit
CAACGGAATCCCAAAAGCAGCT206240.42971196546969326No Hit
AACGGAATCCCAAAAGCAGCTG192610.40131313842667576No Hit
AGCAGCATTGTACAGGGCTATGA190090.39606258493082813No Hit
TTAATGCTAATCGTGATAGGGGTT181420.3779981806415426No Hit
CTCCCACTGCTTCACTTGACTAG169550.3532664068337204No Hit
TGAGAACTGAATTCCATAGGCTGT163270.340181694153592No Hit
CCCATAAAGTAGAAAGCACT162250.3380564701195584No Hit
ACTGGACTTGGAGTCAGAAGGC161950.3374314042271956No Hit
TGTAAACATCCTTGACTGGAAGCT161240.3359520816152703No Hit
TTCTCACTACTGCACTTGACTAG159500.33232669943956594No Hit
ACCGGGTGCTGTAGGCTTT157500.32815959349048046No Hit
ACCGGGTGCTGTAGGCTTA138130.2878011723735877No Hit
TACCACCCTGAACGCGCCCGA135450.28221725040181317No Hit
TCGTTTCCCGGCCAATGCACCA131540.27407055827135113No Hit
CCCATAAAGTAGAAAGCACTAC130480.27186199211833584No Hit
CAACACCAGTCGATGGGCTGT128190.26709065580663294No Hit
TTCACAGTGGCTAAGTTCCGC119870.24975549505843742No Hit
AACCGGGCGGAAACACCA118960.2478594618516035No Hit
TGAGGTAGTAGATTGTATAGT113490.23646242708085474No Hit
TGTAAACATCCCCGACTGGAAGCT99540.20739686308598365No Hit
CCACCCTGAACGCGCCCGA96640.2013545594598097No Hit
TGGCTCAGTTCAGCAGGAAC96510.20108369757311917No Hit
TAGCAGCACGTAAATATTGGC88760.184936162020413No Hit
ACCACCCTGAACGCGCCCGA83610.17420586420151793No Hit
CTCGTACGACTCTTAGCGG79130.1648715468755665No Hit
TGATGTAGTAGATTGTATAGTT78800.16418397439396737No Hit
TGTAAACATCCCCGACTGGA77380.16122532917011667No Hit
AGCTCGGTCTGAGGCCCCTCAGT74280.1547663149490342No Hit
CACTAGATTGTGAGCTCCTGGA74110.15441211094336194No Hit
TCCCACTGCTTCACTTGACTAG74110.15441211094336194No Hit
TCGTTTCCCGGCCAACGCACCA73230.15257858432576435No Hit
GGAATACCGGGTGCTGTAGGCT71420.148807353441842No Hit
CTTCTCACTACTGCACTTGACTAG71390.1487448468526057No Hit
CCCATAAAGTAGAAAGCACTA70240.14634876093188157No Hit
CCCCCACTGCTAAATTTGACTGGCTT68300.14230666816126866No Hit
TATTGCACTTGTCCCGGCCTGTT66660.1388896412830186No Hit
GGAATACCGGGTGCTGTAGGCTTA64980.1353892722857868No Hit
TTCACAGTGGCTAAGTTCTGC64630.13466002874469685No Hit
TGAGAACTGAATTCCATGGGTTG61960.12909694230266774No Hit
TTCAAGTAATCCAGGATAGGC61120.12734675780405183No Hit
TGAGGTAGTAGTTTGTGCTGTT58220.12130445417787791No Hit
TATTGCACTTGTCCCGGCCTGTAG56440.11759572988319185No Hit
AATGCACCCGGGCAAGGATTCT56320.11734570352624674No Hit
AGGTCCCTGTTCGGGCGCCA55510.11565802561686711No Hit
ATCCCGGACGAGCCCCCA54990.11457457807010488No Hit
TACCACCCTGAACGCGCCCG54980.11455374254035947No Hit
CTCCCACTGCTTCACTTGACTAGCCT53910.11232434085759874No Hit
TAGCTTATCAGACTGATGTTGACA52690.10978240622865659No Hit
CACTAGATTGTGAGCTCCTGGAA52270.10890731397934865No Hit
ATCCCACCGCTGCCACCA52260.10888647844960322No Hit
TGAGGTAGTAAGTTGTATTGTT51490.10728214265920533No Hit
TTCACAGTGGCTAAGTTCTG50820.10588616216626169No Hit
TCGGACGACTCTTAGCGG49810.10378177366197354No Hit
CAAAGTGCTGTTCGTGCAGGTAG49540.103219214358847No Hit
ACTGGACTTGGAGTCAGAAGGCT49120.10234412210953904No Hit
TCCCCGGCACCTCCACCA48170.10036474678372345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)