FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031031
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4976296.196327719317731No Hit
CCCCCCACTGCTAAATTTGACTG3982704.959139119248824No Hit
AACATTCAACGCTGTCGGTGAGT3348254.169140923500358No Hit
AAGCTGCCAGTTGAAGAACTGT2547783.1724195809977576No Hit
TAGCAGCACGTAAATATTGGCG2542023.1652474009874947No Hit
TTCAAGTAATCCAGGATAGGCT2464093.068211291925034No Hit
AACATTCAACGCTGTCGGTGAG1758882.190104857022716No Hit
TAGCTTATCAGACTGATGTTGAC1630612.0303868830788976No Hit
TAGCTTATCAGACTGATGTTGACT1231181.5330285737908371No Hit
TATTGCACTTGTCCCGGCCTGTAA1207371.50338107274147No Hit
CCCCCACTGCTAAATTTGACTG1139281.4185974378632082No Hit
GCCTGTCTGAGCGTCGCT1113241.3861732074001458No Hit
CGCCTGTCTGAGCGTCGCT967851.2051379206480464No Hit
TATTGCACTTGTCCCGGCCTGTAT963111.1992358141812676No Hit
TGTAAACATCCTTGACTGGAAGCT856981.0670859071518963No Hit
TGAGGTAGTAGGTTGTATAGTT770320.9591794627613814No Hit
TAGCTTATCAGACTGATGTTGA759950.9462670484026273No Hit
AGCAGCATTGTACAGGGCTATGA596550.7428062474170501No Hit
TATTGCACTTGTCCCGGCCTGT585360.7288727935429461No Hit
TATTGCACTTGTCCCGGCCTGTA503010.6263330324587216No Hit
ACTGGACTTGGAGTCAGAAGGC479910.5975696022092307No Hit
TCCTGTCTGAGCGTCGCT471390.586960752610717No Hit
TGAGGTAGTAGATTGTATAGTT450640.561123472191802No Hit
TACGCCTGTCTGAGCGTCGCT423370.5271676824557145No Hit
TGTAAACATCCCCGACTGGAAGCT420310.5233574618252625No Hit
CTACGCCTGTCTGAGCGTCGCT384520.4787928224906615No Hit
CATAAAGTAGAAAGCACTACT381680.47525653929115697No Hit
CCCCCACTGCTAAATTTGACTGGCTT367920.4581229981555295No Hit
TTCAAGTAATTCAGGATAGGTT333390.4151272731981734No Hit
AACATTCAACGCTGTCGGTGAGTT321190.399936197481992No Hit
TTCTCACTACTGCACTTGACTAG290070.36118650270432273No Hit
CAACGGAATCCCAAAAGCAGCTGT273630.340715905591698No Hit
CTCCCACTGCTTCACTTGACTAG259650.3233084270251229No Hit
CCCATAAAGTAGAAAGCACTAC254930.31743122396115764No Hit
ACGCCTGTCTGAGCGTCGCT250860.3123633814886283No Hit
TGTAAACATCCTACACTCTCAGCT235200.2928640170857266No Hit
CTTCTCACTACTGCACTTGACTAG232030.28891682773980076No Hit
TAGCTTATCAGACTGATGTTGACA212070.2640632317320155No Hit
CCCCCCACTGCTAAATTTGACTGGCT209710.26112463020003285No Hit
TGTCAGTTTGTCAAATACCCCAA197130.2454603898303966No Hit
GTCAGGATGGCCGAGCGGTCTAAGG195700.24367979652923766No Hit
TAGCAGCACATCATGGTTTACA193880.24141358687321718No Hit
TGAGGTAGTAGTTTGTACAGTT191590.23856214725108144No Hit
GCGTTCGCGCTTTCCCCTG189290.2356982559275391No Hit
CATTGCACTTGTCTCGGTCTGA188170.234303665369988No Hit
CAAAGAATTCTCCTTTTGGGCT182380.22709413025550515No Hit
TTCACAGTGGCTAAGTTCCG177250.22070640743386497No Hit
AACCGGGCGGAAACACCA174080.21675921808793916No Hit
CTCCCACTGCTTCACTTGACTAGCCT170250.2119902164491707No Hit
CACCCGGCTGTGTGCACATGTGC169200.21068278780146657No Hit
CCCCCCACTGCTAAATTTGACTGG166860.20776908967229737No Hit
CCAAGTCCTTCTGATCGAGGCCC161230.20075878178032186No Hit
ACTGGACTTGGAGTCAGAAGGCA151360.18846895249190296No Hit
GGGCTACGCCTGTCTGAGCGTCGCT146890.18290304196310536No Hit
TAGCAGCACGTAAATATTGGC146280.18214348817729628No Hit
TTCACAGTGGCTAAGTTCCGC136740.1702645650352987No Hit
TCCACCACGTTCCCGTGG134350.16728860839909596No Hit
CGCGACCTCAGATCAGACGTGGCG133780.1665788614189137No Hit
CCCATAAAGTAGAAAGCACT133330.16601853485561194No Hit
TATTGCACTTGTCCCGGCCTGTAG130050.16193437679421233No Hit
ACCGGGTGCTGTAGGCTT129830.16166043936326482No Hit
TAGCAGCACGTAAATATTGGCGT129340.16105030599433623No Hit
ATCACATTGCCAGGGATTTCCA128870.16046507602822102No Hit
TGAGGTAGTAGTTTGTGCTGTT127770.15909538887348337No Hit
CAACACCAGTCGATGGGCTGT126980.1581117044623536No Hit
TTCAAGTAATCCAGGATAGGCTT126130.15705330984278357No Hit
GGAATACCGGGTGCTGTAGGCTTT124670.1552353614374045No Hit
TGTAAACATCCTACACTCAGCT124300.15477464848535635No Hit
TCGTACGACTCTTAGCGG123300.15352947834468575No Hit
TGTCAGTTTGTCAAATACCCCA120510.1500554536522148No Hit
CAAAGAATTCTCCTTTTGGGCTT120470.15000564684658793No Hit
TATTGCACTTGTCCCGGCCTGTT118430.1474654997596199No Hit
TGTAAACATCCCCGACTGGAAGC118340.14735343444695956No Hit
AACGGAATCCCAAAAGCAGCTG115080.1432941797883734No Hit
ACTGGACTTGGAGTCAGAAGG114970.1431572110728996No Hit
AGCAGCATTGTACAGGGCTATGT109680.13657026102875208No Hit
TTATAAAGCAATGAGACTGATT106560.1326853301898598No Hit
ACTGGACTTGGAGTCAGAAGGCT106370.13244874786313238No Hit
AACATTCAACGCTGTCGGTGAGA105750.13167674237591662No Hit
TGAGGTAGTAGGTTGTGTGGTT104520.13014518310289178No Hit
CAAGTCCTTCTGATCGAGGCCC104030.12953504973396318No Hit
TTCAAGTAATCCAGGATAGGC103430.1287879476495608No Hit
ATCACATTGCCAGGGATTTCC101110.125899152923205No Hit
GGAATACCGGGTGCTGTAGGCTT98780.12299790649544248No Hit
AGCTCGGTCTGAGGCCCCTCAGT98630.12281113097434189No Hit
TCAGGCTCAGTCCCCTCCCGAT98130.1221885459040066No Hit
CACTAGATTGTGAGCTCCTGGAA96030.11957368860859832No Hit
CCTCGACACAAGGGTTTGT95210.11855264909324842No Hit
CAAAGTGCTGTTCGTGCAGGTAG94250.11735728575820464No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT92200.1148046869698299No Hit
ACCCCCCACTGCTAAATTTGACTG91820.11433152231637507No Hit
CAACGGAATCCCAAAAGCAGCT90600.11281241474475692No Hit
ACCACAGGGTAGAACCACGGAC88080.109674585990267No Hit
CTGCGTTCGCGCTTTCCCCTG87110.1084667709538165No Hit
TCGTTTCCCGGCCAATGCACCA86590.1078192824806678No Hit
CACTAGATTGTGAGCTCCTGGA85040.10588926876262837No Hit
CAAAGTGCTGTTCGTGCAGGT84640.10539120070636011No Hit
TCGTTCGCGCTTTCCCCTG81540.10153117327028123No Hit
TATTGCACTTGTCCCGGCCTGTAAT80990.1008463296929124No Hit
TTCACAGTGGCTAAGTTCTGC80800.10060974736618498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)