FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6763239
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG68355810.106962063591128No Hit
CAACGGAATCCCAAAAGCAGCTG4395326.498838796026578No Hit
AACATTCAACGCTGTCGGTGAGT2706954.002446165217584No Hit
TTCAAGTAATCCAGGATAGGCT2014892.9791790590277825No Hit
AAGCTGCCAGTTGAAGAACTGT1893162.799191334211315No Hit
CCCCCACTGCTAAATTTGACTG1802852.665660639820654No Hit
TAGCAGCACGTAAATATTGGCG1559712.306158336264621No Hit
AACATTCAACGCTGTCGGTGAG1458362.1563041022208442No Hit
TAGCTTATCAGACTGATGTTGACT1137571.6819899459415821No Hit
TAGCTTATCAGACTGATGTTGAC1103121.6310528136001108No Hit
TATTGCACTTGTCCCGGCCTGTAA982251.4523366688653174No Hit
TAGCTTATCAGACTGATGTTGA953631.4100196666124027No Hit
TATTGCACTTGTCCCGGCCTGTAT888471.3136752967032512No Hit
TGAGGTAGTAGGTTGTATAGTT813671.2030774012274297No Hit
TGTAAACATCCTTGACTGGAAGCT577510.8538955964738196No Hit
AGCAGCATTGTACAGGGCTATGA545870.8071132781201433No Hit
TATTGCACTTGTCCCGGCCTGT523260.7736825506240428No Hit
TGAGGTAGTAGATTGTATAGTT517820.7656390673167103No Hit
ACTGGACTTGGAGTCAGAAGGC481770.7123362045907294No Hit
TATTGCACTTGTCCCGGCCTGTA412430.6098113640520467No Hit
CGCCTGTCTGAGCGTCGCT370450.5477405130884773No Hit
TGTAAACATCCCCGACTGGAAGCT351430.51961789314262No Hit
TTCAAGTAATTCAGGATAGGTT345440.5107611900156123No Hit
CATAAAGTAGAAAGCACTACT317420.4693313366568888No Hit
GCCTGTCTGAGCGTCGCT303980.44945920142700857No Hit
CAACGGAATCCCAAAAGCAGCTGT276940.4094783579287971No Hit
GGAATACCGGGTGCTGTAGGCTTT270660.40019286616959715No Hit
CCCATAAAGTAGAAAGCACTAC263160.38910350499220864No Hit
AACCGGGCGGAAACACCA237610.3513257479145717No Hit
CTACGCCTGTCTGAGCGTCGCT237000.35042381320547744No Hit
TTCTCACTACTGCACTTGACTAG236600.34983238060935No Hit
AACATTCAACGCTGTCGGTGAGTT233780.3456627808066519No Hit
CCCCCACTGCTAAATTTGACTGGCTT229990.340058956958345No Hit
TGAGGTAGTAGTTTGTACAGTT224320.33167539990823924No Hit
CTTCTCACTACTGCACTTGACTAG219990.3252731420551603No Hit
CACCCGGCTGTGTGCACATGTGC218160.32256733792787745No Hit
CCCCCCACTGCTAAATTTGACTGGCT206370.3051348621570227No Hit
TCGTACGACTCTTAGCGG198770.2938976428306023No Hit
TACGCCTGTCTGAGCGTCGCT195960.2897428288428074No Hit
GGAATACCGGGTGCTGTAGGCTT193300.28580980207856027No Hit
CCCCCCACTGCTAAATTTGACTGG174990.2587369749908291No Hit
ACCCCCCACTGCTAAATTTGACTG174290.25770196794760614No Hit
CTCCCACTGCTTCACTTGACTAG170420.2519798575800737No Hit
CATTGCACTTGTCTCGGTCTGA164970.24392158845783804No Hit
CCCATAAAGTAGAAAGCACT162500.24026949217675142No Hit
TGTCAGTTTGTCAAATACCCCAA153180.22648911268698327No Hit
TAGCTTATCAGACTGATGTTGACA151720.22433038371111827No Hit
TGTAAACATCCTACACTCTCAGCT150170.2220385824011247No Hit
CGCGACCTCAGATCAGACGTGGCG146690.2168931188148164No Hit
ACTGGACTTGGAGTCAGAAGGCA133640.19759763036616035No Hit
TGAGGTAGTAGTTTGTGCTGTT132820.1963851935440992No Hit
ACTGGACTTGGAGTCAGAAGGCT128260.18964286194824698No Hit
CAAAGAATTCTCCTTTTGGGCT125180.1850888309580661No Hit
TTCAAGTAATCCAGGATAGGCTT122770.18152544956639857No Hit
CTCCCACTGCTTCACTTGACTAGCCT118620.17538933638157692No Hit
AGCAGCATTGTACAGGGCTATGT115670.17102752098513743No Hit
AGCTCGGTCTGAGGCCCCTCAGT111470.16481747872579985No Hit
AACGGAATCCCAAAAGCAGCTG111200.1644182617234139No Hit
TGAGGTAGTAGGTTGTGTGGTT110930.1640190447210279No Hit
CACTAGATTGTGAGCTCCTGGA110840.16388597238689925No Hit
TATTGCACTTGTCCCGGCCTGTAG110570.16348675538451324No Hit
CAACACCAGTCGATGGGCTGT107190.1584891499472368No Hit
TATTGCACTTGTCCCGGCCTGTT104390.1543491217743451No Hit
CAACGGAATCCCAAAAGCAGCT102240.15117017157016038No Hit
ACGCCTGTCTGAGCGTCGCT101690.15035695175048522No Hit
GACCCGCGGGCGCTCTCCAGTCCT101370.1498838056735833No Hit
TAGCAGCACGTAAATATTGGC100950.14926280144764958No Hit
CACTAGATTGTGAGCTCCTGGAA98790.14606906542856168No Hit
TGTAAACATCCCCGACTGGAAGC93690.1385282998279375No Hit
TGTCAGTTTGTCAAATACCCCA91770.135689423366526No Hit
CAAAGAATTCTCCTTTTGGGCTT90950.13447698654446485No Hit
TTATAAAGCAATGAGACTGATT90610.13397426883775657No Hit
TTCAAGTAATCCAGGATAGGC86710.12820780102551455No Hit
CCCCCCACTGCTAAATTTGACTGTA81540.12056353472056806No Hit
AACATTCAACGCTGTCGGTGAGA81320.120238246792698No Hit
ACTGGACTTGGAGTCAGAAGG78630.1162608625837413No Hit
TATTGCACTTGTCCCGGCCTGTAAT77410.11445699316555277No Hit
CCCCCCACTGCTAAATTTGACTGT77030.11389513219923177No Hit
TGAGGTAGTAGGTTGTATAGT74760.11053875221620883No Hit
AGCAGCATTGTACAGGGCTATG71120.1051567155914496No Hit
AACATTCAACGCTGTCGGTGA70080.10361899084151839No Hit
CCCCCACTGCTAAATTTGACTGGCT69700.10305712987519738No Hit
ACCGGGTGCTGTAGGCTT69400.10261355542810183No Hit
TATTGCACTTGTCCCGGCCTGTTT69360.10255441216848908No Hit
GGGCTACGCCTGTCTGAGCGTCGCT69020.10205169446178082No Hit
TAGCAGCACGTAAATATTGGCGT68490.10126804627191202No Hit
TTCACAGTGGCTAAGTTCCG68350.10106104486326743No Hit
GTGCGGTAACGCGACCGATCCCGGA67720.1001295385243668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)