FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9232159
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG93568110.13501825520986No Hit
CCCCCCACTGCTAAATTTGACTG5965626.461782124852919No Hit
TCGTACGACTCTTAGCGG3409483.6930473142847733No Hit
TTCAAGTAATCCAGGATAGGCT2974283.2216516201681533No Hit
CCCCCACTGCTAAATTTGACTG2711072.9365503778693585No Hit
TAGCAGCACGTAAATATTGGCG2048772.219166719290688No Hit
TGAGGTAGTAGATTGTATAGTT2025422.1938746938825466No Hit
AACATTCAACGCTGTCGGTGAG1976652.1410484806425023No Hit
AAGCTGCCAGTTGAAGAACTGT1969162.1329355354473423No Hit
TAGCTTATCAGACTGATGTTGA1447791.5682030606275303No Hit
TGAGGTAGTAGGTTGTATAGTT1312061.4211843621844034No Hit
TAGCTTATCAGACTGATGTTGAC1309941.4188880412479898No Hit
TATTGCACTTGTCCCGGCCTGT1123121.2165301745777992No Hit
TGAGAACTGAATTCCATGGGTT897500.9721453020902261No Hit
AACATTCAACGCTGTCGGTGAGT826750.8955110066886846No Hit
TAGCTTATCAGACTGATGTTGACT818770.8868673080695425No Hit
TATTGCACTTGTCCCGGCCTGTAA733410.7944078952713011No Hit
TGAGGTAGTAGTTTGTACAGTT696650.7545905567700903No Hit
TATTGCACTTGTCCCGGCCTGTAT683730.7405959971010031No Hit
TTCAAGTAATTCAGGATAGGTT648250.7021651165236648No Hit
GCCTGTCTGAGCGTCGCT550270.5960360951322438No Hit
CATAAAGTAGAAAGCACTACT547760.5933173377971501No Hit
TTCTCACTACTGCACTTGACTAG511730.5542907135806479No Hit
TATTGCACTTGTCCCGGCCTGTA482680.5228246177302622No Hit
AGCAGCATTGTACAGGGCTATGA481100.5211132087304823No Hit
CAACGGAATCCCAAAAGCAGCTGT436920.4732587469518235No Hit
CATTGCACTTGTCTCGGTCTGA425300.4606723086116693No Hit
TGTAAACATCCTTGACTGGAAGCT417100.4517903125368617No Hit
CCCATAAAGTAGAAAGCACT392600.42525264133774127No Hit
CACCCGGCTGTGTGCACATGTGC386760.41892692706007334No Hit
TTAATGCTAATCGTGATAGGGGTT362450.3925950582090278No Hit
CCCATAAAGTAGAAAGCACTAC322800.3496473576765738No Hit
ACTGGACTTGGAGTCAGAAGGC318770.3452821815568818No Hit
CTCCCACTGCTTCACTTGACTAG296560.3212249702371894No Hit
CAACGGAATCCCAAAAGCAGCT284320.3079669663401594No Hit
TGATGTAGTAGATTGTATAGTT281410.30481494090385575No Hit
TGAGGTAGTAGATTGTATAGT276620.29962655539186445No Hit
ACCGGGTGCTGTAGGCTT268930.29129697614609973No Hit
CCCCCACTGCTAAATTTGACTGGCTT268250.2905604203740425No Hit
AACGGAATCCCAAAAGCAGCTG254670.27585096833795864No Hit
CTTCTCACTACTGCACTTGACTAG253360.2744320153064955No Hit
CAACACCAGTCGATGGGCTGT245870.2663190701113358No Hit
TGAGAACTGAATTCCATAGGCTGT232520.25185874723344776No Hit
TGTAAACATCCCCGACTGGAAGCT220060.23836244588075228No Hit
TTCACAGTGGCTAAGTTCCGC213230.23096439305258934No Hit
GGAATACCGGGTGCTGTAGGCTTT199670.216276604421566No Hit
TGAGGTAGTAGTTTGTGCTGTT189470.2052282678407077No Hit
TGATGTAGTAGGTTGTATAGTT186040.20151299387283084No Hit
ACCGGGTGCTGTAGGCTTT185060.20045148702486604No Hit
TAGCAGCACGTAAATATTGGC177600.19237103693729712No Hit
GGAATACCGGGTGCTGTAGGCTT175800.19042133048185153No Hit
TGGCTCAGTTCAGCAGGAAC171730.18601282755203846No Hit
CCCCCCACTGCTAAATTTGACTGG170640.18483217197624088No Hit
TAGTGGGTTATCAGAACT165840.17963295476171934No Hit
ACCCCCCACTGCTAAATTTGACTG161540.17497532267371046No Hit
TGAGGTAGTAAGTTGTATTGTT159370.1726248432246455No Hit
TCGTTTCCCGGCCAATGCACCA158250.17141169254125713No Hit
TGTAAACATCCCCGACTGGA154270.16710067493421638No Hit
TTCAAGTAATCCAGGATAGGC149040.16143569451089393No Hit
CCCATAAAGTAGAAAGCACTA147060.1592910174099038No Hit
TATTGCACTTGTCCCGGCCTGTT144200.15619315048625138No Hit
TTCACAGTGGCTAAGTTCTGC141630.1534094029359763No Hit
TAGCTTATCAGACTGATGTTGACA140930.15265118375885856No Hit
ACCGGGTGCTGTAGGCTTA135690.1469753716330059No Hit
TCCTGTCTGAGCGTCGCT134140.14529645774081665No Hit
CACTAGATTGTGAGCTCCTGGA132790.14383417789923245No Hit
AACCGGGCGGAAACACCA132280.14328176107018953No Hit
TACCACCCTGAACGCGCCCGA131220.1421336006019827No Hit
AGCTCGGTCTGAGGCCCCTCAGT129530.14030304287436993No Hit
TGTCAGTTTGTCAAATACCCCA124320.13465972585610797No Hit
TTCACAGTGGCTAAGTTCTG122000.13214677086908924No Hit
TGTAAACATCCTACACTCTCAGCT119810.12977462801496378No Hit
TGATAACTGAATTCCATGGGTT118530.12838817009109138No Hit
TTCAAGTAATCCAGGATAGGCTT115130.12470539123080528No Hit
CCCCCCACTGCTAAATTTGACTGGCT112740.12211661432607475No Hit
CTCCCACTGCTTCACTTGACTAGCCT112480.12183499006028817No Hit
CAAAGAATTCTCCTTTTGGGCT110590.1197877982820703No Hit
TGAGAACTGAATTCCATGGGTTG108710.11775143820638272No Hit
TTATAAAGCAATGAGACTGATT107120.11602919750407245No Hit
TTCACAGTGGCTAAGTTCCG105430.11419863977645967No Hit
TCCCACTGCTTCACTTGACTAG104490.11318045973861585No Hit
TAGCTTATCAGACTGATGTTG104210.11287717206776876No Hit
TGAGGTAGTAGGTTGTATAGT103340.11193481394763674No Hit
CGCCTGTCTGAGCGTCGCT100960.10935686874543647No Hit
CAAAGTGCTGTTCGTGCAGGTAG97140.10521915837887973No Hit
TGATGTAGTAGTTTGTACAGTT96160.10415765153091493No Hit
CTCGTACGACTCTTAGCGG95020.10292283744246607No Hit
ACTGGACTTGGAGTCAGAAGGCT94640.10251123274631643No Hit
AGCAGCATTGTACAGGGCTATGT94430.10228376699318112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)