FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10721559
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG152668614.239403056962145No Hit
CAACGGAATCCCAAAAGCAGCTG5009244.672119045373905No Hit
CCCCCACTGCTAAATTTGACTG3667093.420295499936157No Hit
AACATTCAACGCTGTCGGTGAGT3381783.154186811824661No Hit
AAGCTGCCAGTTGAAGAACTGT3351593.126028593416312No Hit
TTCAAGTAATCCAGGATAGGCT2677062.4968943415784963No Hit
TAGCTTATCAGACTGATGTTGAC2591912.417474921324408No Hit
TAGCAGCACGTAAATATTGGCG2515022.345759604550047No Hit
TAGCTTATCAGACTGATGTTGACT2125391.9823516337502782No Hit
AACATTCAACGCTGTCGGTGAG1657651.546090451957593No Hit
TATTGCACTTGTCCCGGCCTGTAA1543941.4400331145871603No Hit
TATTGCACTTGTCCCGGCCTGTAT1470881.3718900394989197No Hit
TAGCTTATCAGACTGATGTTGA1297681.210346368471227No Hit
TGTAAACATCCTTGACTGGAAGCT1096301.022519206395264No Hit
TGAGGTAGTAGGTTGTATAGTT827570.7718746872539711No Hit
AGCAGCATTGTACAGGGCTATGA761520.7102698404215282No Hit
TATTGCACTTGTCCCGGCCTGT757890.7068841387712365No Hit
ACTGGACTTGGAGTCAGAAGGC644130.6007801663918465No Hit
TGAGGTAGTAGATTGTATAGTT610510.5694227863690345No Hit
TTAATGCTAATCGTGATAGGGGTT592130.5522797570763729No Hit
TGTAAACATCCCCGACTGGAAGCT590580.5508340717987001No Hit
TATTGCACTTGTCCCGGCCTGTA584870.5455083537757895No Hit
CGCCTGTCTGAGCGTCGCT577460.5385970454483345No Hit
TTCAAGTAATTCAGGATAGGTT477920.4457560696163683No Hit
TGTAAACATCCTACACTCTCAGCT431370.402338876277228No Hit
CTTCTCACTACTGCACTTGACTAG415240.3872944223876397No Hit
TTCTCACTACTGCACTTGACTAG375970.3506672863526657No Hit
CATAAAGTAGAAAGCACTACT364930.340370276374919No Hit
AACATTCAACGCTGTCGGTGAGTT361190.33688197770492145No Hit
CCCATAAAGTAGAAAGCACTAC356470.33247963285936305No Hit
CAACGGAATCCCAAAAGCAGCTGT347790.32438379530439554No Hit
GGAATACCGGGTGCTGTAGGCTTT328540.3064293168558789No Hit
CCCCCACTGCTAAATTTGACTGGCTT311400.29044283578535546No Hit
TAGCTTATCAGACTGATGTTGACA295760.27585540498354766No Hit
CCCCCCACTGCTAAATTTGACTGG294200.27440039270408345No Hit
CCCCCCACTGCTAAATTTGACTGGCT292740.27303865044253356No Hit
TACGCCTGTCTGAGCGTCGCT273930.25549456007284016No Hit
CTACGCCTGTCTGAGCGTCGCT270550.25234203346733436No Hit
ACCCCCCACTGCTAAATTTGACTG264480.24668054337993198No Hit
AACCGGGCGGAAACACCA259200.24175588643405313No Hit
CAAAGAATTCTCCTTTTGGGCT258130.24075789724236932No Hit
TGAGGTAGTAGTTTGTACAGTT245050.2285581788991694No Hit
CACCCGGCTGTGTGCACATGTGC242760.22642229548893028No Hit
AGCTCGGTCTGAGGCCCCTCAGT234020.21827049592321413No Hit
CTCCCACTGCTTCACTTGACTAG232410.21676884863479276No Hit
CAAAGAATTCTCCTTTTGGGCTT228850.2134484359970411No Hit
GGAATACCGGGTGCTGTAGGCTT225870.21066898946319282No Hit
TTCAAGTAATCCAGGATAGGCTT225720.21052908443632123No Hit
CCCATAAAGTAGAAAGCACT204370.19061593561160275No Hit
TATTGCACTTGTCCCGGCCTGTAG199210.18580320268722114No Hit
CATTGCACTTGTCTCGGTCTGA193190.18018834760877592No Hit
ACTGGACTTGGAGTCAGAAGGCA188120.17545955770051724No Hit
GCCTGTCTGAGCGTCGCT178930.16688804305418642No Hit
CACTAGATTGTGAGCTCCTGGAA177960.165983323880417No Hit
CCAAGTCCTTCTGATCGAGGCCC170810.1593145175995394No Hit
CCCCCCACTGCTAAATTTGACTGT169930.1584937414418929No Hit
CACTAGATTGTGAGCTCCTGGA165300.1541753396124575No Hit
CAACGGAATCCCAAAAGCAGCT165260.15413803160529174No Hit
GCGTTCGCGCTTTCCCCTG165190.15407274259275167No Hit
TGAGGTAGTAGTTTGTGCTGTT159100.1483925985017664No Hit
AGCAGCATTGTACAGGGCTATGT158780.1480941344444404No Hit
ACGCCTGTCTGAGCGTCGCT151350.14116417211340254No Hit
TATTGCACTTGTCCCGGCCTGTT151010.14084705405249368No Hit
CGCGACCTCAGATCAGACGTGGCG149880.13979310285006127No Hit
TGTAAACATCCCCGACTGGAAGC149040.13900963469958053No Hit
TGAATACCGGGTGCTGTAGGCTTT143480.13382382170354146No Hit
TTATAAAGCAATGAGACTGATT140220.1307832191195329No Hit
TGTAAACATCCTACACTCAGCT140180.13074591111236714No Hit
CCCCCCACTGCTAAATTTGACTGTA136280.12710838041370662No Hit
ACTGGACTTGGAGTCAGAAGGCT134470.12542019308945648No Hit
GACCCGCGGGCGCTCTCCAGTCCT131620.12276199757889689No Hit
TAGCAGCACGTAAATATTGGC129740.12100852124210668No Hit
TATTGCACTTGTCCCGGCCTGTTT127910.11930167991427366No Hit
TTCACAGTGGCTAAGTTCCG127570.11898456185336481No Hit
TATTGCACTTGTCCCGGCCTGTAAT125360.11692329445745717No Hit
TTCAAGTAATCCAGGATAGGC125160.11673675442162841No Hit
AACGGAATCCCAAAAGCAGCTG121350.11318316673909085No Hit
CGCCCCACTGCTAAATTTGACTG118190.11023583417299668No Hit
ACTGGACTTGGAGTCAGAAGG116410.10857562785412084No Hit
CAACACCAGTCGATGGGCTGT115920.10811860476634041No Hit
CTCCCACTGCTTCACTTGACTAGCCT111340.10384683794586218No Hit
TGTCAGTTTGTCAAATACCCCA111160.10367895191361629No Hit
ATCACATTGCCAGGGATTTCCA110310.10288615676134413No Hit
GCCCCCACTGCTAAATTTGACTG109670.10228922864669214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)