FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119067
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG151509814.972704499337736No Hit
CAACGGAATCCCAAAAGCAGCTG7125217.041370513704474No Hit
CCCCCACTGCTAAATTTGACTG3930263.884014208029258No Hit
AACATTCAACGCTGTCGGTGAGT2852972.8194002470781148No Hit
AAGCTGCCAGTTGAAGAACTGT2531392.501604149868758No Hit
TTCAAGTAATCCAGGATAGGCT2525622.49590204314291No Hit
TAGCAGCACGTAAATATTGGCG2509382.4798531327048234No Hit
AACATTCAACGCTGTCGGTGAG2110032.0852021238716967No Hit
TATTGCACTTGTCCCGGCCTGTAA1498071.4804428115754151No Hit
TATTGCACTTGTCCCGGCCTGTAT1488991.471469652291066No Hit
TAGCTTATCAGACTGATGTTGACT1236031.2214861310830336No Hit
TAGCTTATCAGACTGATGTTGA1196891.1828066757538023No Hit
TAGCTTATCAGACTGATGTTGAC1144471.1310034808545095No Hit
TATTGCACTTGTCCCGGCCTGT866370.8561757719362862No Hit
TATTGCACTTGTCCCGGCCTGTA783980.7747552219982337No Hit
TGAGGTAGTAGGTTGTATAGTT742000.7332691838091397No Hit
TTAATGCTAATCGTGATAGGGGTT605900.5987706178840401No Hit
TGAGGTAGTAGATTGTATAGTT581480.5746379582228283No Hit
GGAATACCGGGTGCTGTAGGCTTT548870.5424116670044778No Hit
TGTAAACATCCTTGACTGGAAGCT537900.5315707465915583No Hit
CATAAAGTAGAAAGCACTACT536700.5303848665099263No Hit
TCGTACGACTCTTAGCGG515660.5095924357453113No Hit
AGCAGCATTGTACAGGGCTATGA484400.4787002596187969No Hit
ACTGGACTTGGAGTCAGAAGGC462910.45746312382356996No Hit
CCCATAAAGTAGAAAGCACTAC452280.4469582027671128No Hit
TTCAAGTAATTCAGGATAGGTT449890.44459632493786233No Hit
TTCTCACTACTGCACTTGACTAG425080.42007825425012013No Hit
CAACGGAATCCCAAAAGCAGCTGT411170.4063319276372021No Hit
CCCCCACTGCTAAATTTGACTGGCTT399110.3944138328168002No Hit
TGTAAACATCCCCGACTGGAAGCT388270.38370138274605753No Hit
GGAATACCGGGTGCTGTAGGCTT380330.3758548095392589No Hit
CTTCTCACTACTGCACTTGACTAG362820.3585508426814448No Hit
CCCATAAAGTAGAAAGCACT332480.32856784128418165No Hit
CACCCGGCTGTGTGCACATGTGC328940.3250694950433672No Hit
CAAAGAATTCTCCTTTTGGGCT322380.31858668393044537No Hit
CTCCCACTGCTTCACTTGACTAG288350.28495710128216367No Hit
CATTGCACTTGTCTCGGTCTGA284900.2815476960474716No Hit
ACCCCCCACTGCTAAATTTGACTG284520.28117216735495476No Hit
CAACGGAATCCCAAAAGCAGCT281960.2786422898474731No Hit
ATCCCGGACGAGCCCCCA281700.27838534916311947No Hit
CCCCCCACTGCTAAATTTGACTGG274360.2711317159971369No Hit
CCCCCCACTGCTAAATTTGACTGGCT271830.268631485491696No Hit
CACTAGATTGTGAGCTCCTGGA259850.2567924493434029No Hit
CACTAGATTGTGAGCTCCTGGAA254030.25104093094748753No Hit
AACATTCAACGCTGTCGGTGAGTT253860.25087293126925636No Hit
AACCGGGCGGAAACACCA242470.23961695282776566No Hit
AGCTCGGTCTGAGGCCCCTCAGT233780.231029204569947No Hit
TTCACAGTGGCTAAGTTCCG226640.2239732180842364No Hit
CGCCTGTCTGAGCGTCGCT223690.22105792955022435No Hit
TATTGCACTTGTCCCGGCCTGTAG209230.20676807456655838No Hit
TGAGGTAGTAGTTTGTACAGTT208820.20636289887200074No Hit
GACCCGCGGGCGCTCTCCAGTCCT206930.2044951377434303No Hit
TGTAAACATCCTACACTCTCAGCT198570.19623350650806048No Hit
TATTGCACTTGTCCCGGCCTGTT195500.19319962996588522No Hit
CCCCCCACTGCTAAATTTGACTGT193670.19139116284139635No Hit
TCGTTTCCCGGGCGGCGCACCA186960.18476011671827058No Hit
TGAGAACTGAATTCCATGGGTT184030.1818645928522857No Hit
CAACACCAGTCGATGGGCTGT180560.17843542294956638No Hit
ACTGGACTTGGAGTCAGAAGGCA170070.1680688545692997No Hit
ACCGGGTGCTGTAGGCTTA168970.16698179782780367No Hit
CTACGCCTGTCTGAGCGTCGCT168520.16653709279719167No Hit
AACGGAATCCCAAAAGCAGCTG163280.16135874977406514No Hit
GCCTGTCTGAGCGTCGCT161910.1600048700142019No Hit
CAAAGAATTCTCCTTTTGGGCTT151520.1497371249740712No Hit
ACTGGACTTGGAGTCAGAAGGCT148130.14638701374346075No Hit
TGAATACCGGGTGCTGTAGGCTTT146130.14441054694074068No Hit
TATTGCACTTGTCCCGGCCTGTTT145580.14386701856999268No Hit
TAGCAGCACGTAAATATTGGC144660.14295784384074145No Hit
CCCATAAAGTAGAAAGCACTA144110.14241431546999345No Hit
TCGTTTCCCGGCCAATGCACCA142040.14036867232917818No Hit
TAGCTTATCAGACTGATGTTGACA140570.13891596922917893No Hit
TGAGGTAGTAGTTTGTGCTGTT138870.1372359724468669No Hit
TTCAAGTAATCCAGGATAGGC130770.12923128189585067No Hit
CCCCCCACTGCTAAATTTGACTGA129580.12805528414823225No Hit
TGTCAGTTTGTCAAATACCCCAA128670.1271559917529946No Hit
ACTGGACTTGGAGTCAGAAGG123430.12197764872986809No Hit
CCCCCCACTGCTAAATTTGACTGTA122740.12129576768292966No Hit
TTCACAGTGGCTAAGTTCTG120240.1188251841795296No Hit
CCCCCACAACCGCGCTTGACTAG119660.11825200880674078No Hit
ACCGGGTGCTGTAGGCTT118760.11736259874551676No Hit
TGTAAACATCCCCGACTGGAAGC118510.11711554039517676No Hit
TTCAAGTAATCCAGGATAGGCTT117910.11652260035436074No Hit
TACGCCTGTCTGAGCGTCGCT117520.1161371893278303No Hit
TATTGCACTTGTCCCGGCCTGA114280.11293531310742383No Hit
GGAATACCGGGTGCTGTAGGCTTA104090.10286521474756516No Hit
TGTCAGTTTGTCAAATACCCCA103940.10271697973736117No Hit
ACCGGGTGCTGTAGGCTTT103880.10265768573327956No Hit
TATTGCACTTGTCCCGGCCTGTAAT103690.10246992138702114No Hit
TGAATACCGGGTGCTGTAGGCTT102320.10111604162715791No Hit
CTCCCACTGCTTCACTTGACTAGCCT102240.1010369829550491No Hit
CCCCCACTGCTAAATTTGACTGGCT101390.1001969845638931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)