FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8334997
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6998578.396607701238525No Hit
CAACGGAATCCCAAAAGCAGCTG3920114.703193054538592No Hit
AAGCTGCCAGTTGAAGAACTGT2961313.5528627064892766No Hit
TTCAAGTAATCCAGGATAGGCT2797323.3561139854039537No Hit
AACATTCAACGCTGTCGGTGAGT2689043.2262039206492816No Hit
CCCCCACTGCTAAATTTGACTG2375232.849707084477655No Hit
TAGCAGCACGTAAATATTGGCG2259962.711410693969056No Hit
TAGCTTATCAGACTGATGTTGAC1623191.9474392132354696No Hit
TAGCTTATCAGACTGATGTTGA1621901.9458915222165047No Hit
GGAATACCGGGTGCTGTAGGCTTT1430781.7165932993137252No Hit
AACATTCAACGCTGTCGGTGAG1370861.6447036513630418No Hit
TAGCTTATCAGACTGATGTTGACT1303121.5634318764601836No Hit
GGAATACCGGGTGCTGTAGGCTT1145651.374505593703273No Hit
GCCTGTCTGAGCGTCGCT857421.0286986306053858No Hit
TGAGGTAGTAGGTTGTATAGTT823230.987678819800415No Hit
TATTGCACTTGTCCCGGCCTGTAA736200.883263665241871No Hit
TATTGCACTTGTCCCGGCCTGTAT704660.8454232197084175No Hit
CATAAAGTAGAAAGCACTACT683590.8201442663986561No Hit
TGTAAACATCCTTGACTGGAAGCT670730.8047153466281992No Hit
TATTGCACTTGTCCCGGCCTGT650240.7801322543967322No Hit
TGAGGTAGTAGATTGTATAGTT624180.7488664962926802No Hit
TTCTCACTACTGCACTTGACTAG591600.7097782998602159No Hit
CGCCTGTCTGAGCGTCGCT540940.6489984339526457No Hit
TTCAAGTAATTCAGGATAGGTT512350.6146972818346546No Hit
AGCAGCATTGTACAGGGCTATGA456910.5481825608335552No Hit
TATTGCACTTGTCCCGGCCTGTA415210.4981525488251526No Hit
CTTCTCACTACTGCACTTGACTAG400990.48109195480214334No Hit
CCCATAAAGTAGAAAGCACTAC374790.4496582302309167No Hit
ACTGGACTTGGAGTCAGAAGGC326240.391409858935762No Hit
TGTAAACATCCCCGACTGGAAGCT325950.3910619283966149No Hit
AACCGGGCGGAAACACCA313960.3766768002435994No Hit
CCCATAAAGTAGAAAGCACT303740.36441524813986137No Hit
TGTAAACATCCTACACTCTCAGCT292280.35066599304114926No Hit
CTCCCACTGCTTCACTTGACTAG287500.34493113794762015No Hit
TGAGGTAGTAGTTTGTACAGTT266380.319592196613868No Hit
GGAATACCGGGTGCTGTAGGCTTA266180.31935224451790445No Hit
CTACGCCTGTCTGAGCGTCGCT265790.31888433793077553No Hit
TTCACAGTGGCTAAGTTCCG248240.29782854150997295No Hit
AACATTCAACGCTGTCGGTGAGTT246570.2958249415086772No Hit
CATTGCACTTGTCTCGGTCTGA233690.2803720265286238No Hit
TCGTACGACTCTTAGCGG233210.2797961414983113No Hit
CAACGGAATCCCAAAAGCAGCTGT230330.276340831316436No Hit
TACGCCTGTCTGAGCGTCGCT225580.27064196903730137No Hit
CCCCCACTGCTAAATTTGACTGGCTT204250.24505107800278753No Hit
CCCCCCACTGCTAAATTTGACTGG188360.22598688397848254No Hit
TGTCAGTTTGTCAAATACCCCAA187110.22448718337871026No Hit
ACGCCTGTCTGAGCGTCGCT172060.20643078815745225No Hit
AGCTCGGTCTGAGGCCCCTCAGT169180.20297547797557694No Hit
TGTAAACATCCTACACTCAGCT164620.19750457018760775No Hit
GACCCGCGGGCGCTCTCCAGTCCT157180.18857835221776326No Hit
TTCAAGTAATCCAGGATAGGC155220.18622682167732033No Hit
TGTCAGTTTGTCAAATACCCCA154040.18481110431113532No Hit
CCCCCCACTGCTAAATTTGACTGGCT153890.18463114023916266No Hit
CAAAGAATTCTCCTTTTGGGCT153170.18376731269369384No Hit
GCGTTCGCGCTTTCCCCTG151850.18218362886033435No Hit
GGAATACCGGGTGCTGTAGGCT151720.182027659997958No Hit
TGAGGTAGTAGTTTGTGCTGTT151610.18189568634517803No Hit
TAGCTTATCAGACTGATGTTGACA151270.18148776778204No Hit
TTATAAAGCAATGAGACTGATT150210.18021602167343312No Hit
CACCCGGCTGTGTGCACATGTGC148970.17872831867845904No Hit
TTCAAGTAATCCAGGATAGGCTT144610.17349736298645338No Hit
CAACACCAGTCGATGGGCTGT143080.17166172945233216No Hit
TGATGTAGTAGGTTGTATAGTT140180.16818242406086048No Hit
ACCCCCCACTGCTAAATTTGACTG138330.16596286717319753No Hit
TGAATACCGGGTGCTGTAGGCTTT124960.14992206955803344No Hit
GGGCTACGCCTGTCTGAGCGTCGCT123830.1485663402158393No Hit
TAGCAGCACGTAAATATTGGC122410.146862680334498No Hit
TGTCAGTTTGTCAAATACC116810.1401440216475183No Hit
TATTGCACTTGTCCCGGCCTGTT116750.14007203601872922No Hit
TAGCTTATCAGACTGATGTTG114150.1369526587712029No Hit
ACCGGGTGCTGTAGGCTT109070.13085787553372843No Hit
AGCAGCATTGTACAGGGCTATGT107410.12886627313723087No Hit
TTCACAGTGGCTAAGTTCTG105930.12709062762710052No Hit
TGTAAACATCCCCGACTGGAAGC105870.12701864199831148No Hit
TGATGTAGTAGATTGTATAGTT105780.12691066355512787No Hit
TAGCAGCACGTAAATATTGGCGT104750.1256749102609155No Hit
TGAATACCGGGTGCTGTAGGCTT101320.12155973181514042No Hit
CAAAGAATTCTCCTTTTGGGCTT100260.12028798570653354No Hit
AACATTCAACGCTGTCGGTGA100040.12002403840097363No Hit
GTCAGGATGGCCGAGCGGTCTAAGG99690.1196041222330374No Hit
ACTGGACTTGGAGTCAGAAGG98620.11832037851963234No Hit
TGAGGTAGTAGGTTGTATAGT97830.11737256774057628No Hit
AACGGAATCCCAAAAGCAGCTG95510.11458912342739895No Hit
TGTCAGTTTGTCAAATACCCC94610.11350933899556293No Hit
AGCAGCATTGTACAGGGCTATG91310.10955012941216416No Hit
TTCACAGTGGCTAAGTTCTGC90150.1081584072555755No Hit
CAACGGAATCCCAAAAGCAGCT90010.107990440788401No Hit
TAGCAGCACATAATGGTTTGT89990.10796644557880465No Hit
TCCCACTGCTTCACTTGACTAG89500.10737856294369393No Hit
GCTTGACTAGCTTGCTGTTT89190.10700663719495039No Hit
CACTAGATTGTGAGCTCCTGGA89110.10691065635656496No Hit
TGAGGTAGTAGGTTGTGTGGTT88280.1059148551583162No Hit
TATTGCACTTGTCCCGGCCTGTAG86530.10381527431863502No Hit
ATCCCGGACGAGCCCCCA84720.10164370785016479No Hit
TAGTGGGTTATCAGAACT84210.10103183000545772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)