FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10292156
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6854336.659761084072182No Hit
GCCTGTCTGAGCGTCGCT6673836.484384807226008No Hit
AACATTCAACGCTGTCGGTGAGT4607994.477186315481421No Hit
AAGCTGCCAGTTGAAGAACTGT3709773.604463437981313No Hit
TAGCAGCACGTAAATATTGGCG3132073.0431621907013455No Hit
TCCTGTCTGAGCGTCGCT2916282.8334976656008712No Hit
TTCAAGTAATCCAGGATAGGCT2703852.6270977626067853No Hit
CCCCCCACTGCTAAATTTGACTG2497862.4269550519832777No Hit
CGCCTGTCTGAGCGTCGCT1897761.843889657327386No Hit
AACATTCAACGCTGTCGGTGAG1774941.7245560599742173No Hit
TAGCTTATCAGACTGATGTTGAC1316591.2792169104315947No Hit
TATTGCACTTGTCCCGGCCTGT1238701.203537917614152No Hit
CCCCCACTGCTAAATTTGACTG1103291.0719717034992475No Hit
TAGCTTATCAGACTGATGTTGACT1009450.9807954718136802No Hit
TATTGCACTTGTCCCGGCCTGTAA948150.9212355506465313No Hit
TAGCTTATCAGACTGATGTTGA937340.9107324063102036No Hit
CATAAAGTAGAAAGCACTACT864080.8395519850262666No Hit
TCGTACGACTCTTAGCGG859790.8353837621582884No Hit
TGAGGTAGTAGGTTGTATAGTT858730.8343538516128205No Hit
TATTGCACTTGTCCCGGCCTGTAT852240.8280480785561354No Hit
ACTGGACTTGGAGTCAGAAGGC611900.594530436577137No Hit
AGCAGCATTGTACAGGGCTATGA600560.5835123369680755No Hit
TGTAAACATCCTTGACTGGAAGCT557260.5414414627994367No Hit
TATTGCACTTGTCCCGGCCTGTA556250.540460132940076No Hit
ACCGGGTGCTGTAGGCTT454280.44138468169351497No Hit
CAACGGAATCCCAAAAGCAGCTGT444000.43139649262992125No Hit
CCCATAAAGTAGAAAGCACTAC424910.4128483866742789No Hit
TACGCCTGTCTGAGCGTCGCT404060.3925902405676711No Hit
ACGCCTGTCTGAGCGTCGCT400130.3887717986396631No Hit
TGAGGTAGTAGATTGTATAGTT392060.3809308759019976No Hit
GGAATACCGGGTGCTGTAGGCTTT380000.3692132144129957No Hit
CAACACCAGTCGATGGGCTGT373810.3631989254729524No Hit
AACATTCAACGCTGTCGGTGAGTT371830.36127513030311625No Hit
TGTAAACATCCCCGACTGGAAGCT368670.35820483094115557No Hit
TTCAAGTAATTCAGGATAGGTT366920.35650450692741154No Hit
GCGTTCGCGCTTTCCCCTG364050.3537159755448713No Hit
CATTGCACTTGTCTCGGTCTGA348850.33894744696835144No Hit
GGAATACCGGGTGCTGTAGGCTT338420.32881351584643687No Hit
CGCGACCTCAGATCAGACGTGG328330.3190099333900497No Hit
CTACGCCTGTCTGAGCGTCGCT323670.3144822134448798No Hit
AACCGGGCGGAAACACCA311640.30279370036754205No Hit
CCCATAAAGTAGAAAGCACT296760.2883360881821069No Hit
ATCCCGGACGAGCCCCCA292020.2837306391391658No Hit
ACCGGGTGCTGTAGGCTTT274730.2669314378833745No Hit
ACCGGGTGCTGTAGGCTTA247900.2408630417183727No Hit
TGTAAACATCCTACACTCTCAGCT226760.2203231276323445No Hit
ATCCCACCGCTGCCACCA217030.2108693261159275No Hit
TACCTGGTTGATCCTGCC197500.1918937101225438No Hit
TGAGGTAGTAGTTTGTACAGTT195340.1897950244827226No Hit
ACCACAGGGTAGAACCACGGAC192310.18685103490464No Hit
CACCCGGCTGTGTGCACATGTGC187850.182517637703898No Hit
TGAGGTAGTAGTTTGTGCTGTT185250.1799914420263354No Hit
TGATGTAGTAGGTTGTATAGTT181070.17593009666779244No Hit
CAACGGAATCCCAAAAGCAGCT173740.16880816808451019No Hit
TCGTTCGCGCTTTCCCCTG168760.16396953174825565No Hit
AGCTCGGTCTGAGGCCCCTCAGT166390.16166680722678514No Hit
TATTGCACTTGTCCCGGCCTGTT166020.16130731014959354No Hit
TGAATACCGGGTGCTGTAGGCTTT164900.16021910278079735No Hit
TGTCAGTTTGTCAAATACCCCAA159510.15498210481846564No Hit
TGAGGTAGTAGGTTGTGTGGTT157560.15308745806029367No Hit
TTCAAGTAATCCAGGATAGGCTT157380.15291256759030858No Hit
AACGGAATCCCAAAAGCAGCTG156410.1519701022798333No Hit
CACTAGATTGTGAGCTCCTGGA155890.15146486314432078No Hit
CGCGACCTCAGATCAGACGTGGCG155440.15102763696935803No Hit
TTATAAAGCAATGAGACTGATT151030.14674282045472298No Hit
TTCTCACTACTGCACTTGACTAG148480.14426520546326735No Hit
TGAATACCGGGTGCTGTAGGCTT147740.14354621130888418No Hit
ACTTGACTTGGAGTCAGAAGGC142930.13887274930539337No Hit
CCCTGTCTGAGCGTCGCT142590.13854240063986592No Hit
TTCACAGTGGCTAAGTTCCG141980.13794971626936084No Hit
CCTCGACACAAGGGTTTGT141040.1370363993705498No Hit
CAAAGTGCTGTTCGTGCAGGT138940.1349960105540569No Hit
TTCAAGTAATCCAGGATAGGC131320.12759231399135418No Hit
AACATTCAACGCTGTCGGTGA129290.12561993813541109No Hit
TAGCAGCACGTAAATATTGGC125600.12203468350071647No Hit
ACTGGACTTGGAGTCAGAAGGCA125220.12166547028630346No Hit
AGCAGCATTGTACAGGGCTATGT123690.12017890129143009No Hit
TAGCAGCACGTAAATATTGGCGT121950.11848829341490742No Hit
AGCAGCATTGTACAGGGCTATG120530.11710860192946938No Hit
TTCACAGTGGCTAAGTTCCGC118440.11507792925019793No Hit
ACTGGACTTGGAGTCAGAAGG117690.11434921895859332No Hit
CGCGACCTCAGATCAGACGT116150.11285293382649855No Hit
CCAAGTCCTTCTGATCGAGGCCC115780.11249343674930695No Hit
ACTGGACTTGGAGTCAGAAGGCT114990.11172586190881678No Hit
AACATTCAACGCTGTCGGTGAGA114830.11157040371327445No Hit
TGTAAACATCCTACACTCAGCT113660.11043361565837129No Hit
TGTAAACATCCCCGACTGGAAGC112370.10918023395681138No Hit
TTCACAGTGGCTAAGTTCTGC111910.10873329164462722No Hit
CTCCCACTGCTTCACTTGACTAG110940.10779082633415193No Hit
CGCGACCTCAGATCAGACGTG109250.10614879914373626No Hit
CAAAGTGCTGTTCGTGCAGGTAG108460.10538122430324608No Hit
TATTGCACTTGTCCCGGCCTGTAG108220.10514803700993262No Hit
CGCGACCTCAGATCAGACGTGGC105670.10267042201847698No Hit
CCCCCCACTGCTAAATTTGACTGGCT103410.10047457500644179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)