FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347166
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG77068612.142206458756553No Hit
CCCCCCACTGCTAAATTTGACTG4309636.789849201990306No Hit
AACATTCAACGCTGTCGGTGAGT2075363.269742748180841No Hit
TATTGCACTTGTCCCGGCCTGTAT1726942.720804844240721No Hit
AACATTCAACGCTGTCGGTGAG1658342.612725112278456No Hit
TAGCAGCACGTAAATATTGGCG1611932.539605865042761No Hit
TATTGCACTTGTCCCGGCCTGTAA1590022.505086522079303No Hit
TTCAAGTAATCCAGGATAGGCT1548342.4394194196275945No Hit
AAGCTGCCAGTTGAAGAACTGT1491882.3504663341087975No Hit
CCCCCACTGCTAAATTTGACTG1429282.251839639927489No Hit
TAGCTTATCAGACTGATGTTGACT1096401.7273851038400445No Hit
TAGCTTATCAGACTGATGTTGAC1029941.6226769553529874No Hit
TATTGCACTTGTCCCGGCCTGT734061.1565161522481058No Hit
TATTGCACTTGTCCCGGCCTGTA685761.080419198111409No Hit
TAGCTTATCAGACTGATGTTGA546730.8613765576636879No Hit
TGAGGTAGTAGGTTGTATAGTT476990.7515007485230416No Hit
TGTAAACATCCTTGACTGGAAGCT442490.697145781282544No Hit
AGCAGCATTGTACAGGGCTATGA421910.6647218616938647No Hit
CAACGGAATCCCAAAAGCAGCTGT401410.6324239826089313No Hit
TGTAAACATCCCCGACTGGAAGCT343300.5408713117003715No Hit
TTCAAGTAATTCAGGATAGGTT327640.5161988830920761No Hit
ACTGGACTTGGAGTCAGAAGGC323830.510196204101169No Hit
CCCCCACTGCTAAATTTGACTGGCTT310220.48875356340136683No Hit
TGAGGTAGTAGATTGTATAGTT292350.46059926587708594No Hit
CCCATAAAGTAGAAAGCACTAC278360.43855793278448996No Hit
CATAAAGTAGAAAGCACTACT247890.3905522559202012No Hit
GCCTGTCTGAGCGTCGCT245250.38639291929658054No Hit
CACCCGGCTGTGTGCACATGTGC237920.3748444581408458No Hit
CACTAGATTGTGAGCTCCTGGAA237820.37468690751116324No Hit
TATTGCACTTGTCCCGGCCTGTAG227360.35820711164636315No Hit
CACTAGATTGTGAGCTCCTGGA209080.32940685654038354No Hit
TCGTACGACTCTTAGCGG194770.30686136143280324No Hit
CATTGCACTTGTCTCGGTCTGA192740.3036630836502464No Hit
CAACGGAATCCCAAAAGCAGCT184660.2909329927718922No Hit
ACCGGGTGCTGTAGGCTTA179720.2831499916655717No Hit
TTAATGCTAATCGTGATAGGGGTT170970.26936431156834406No Hit
TATTGCACTTGTCCCGGCCTGTT170510.2686395786718041No Hit
AGCTCGGTCTGAGGCCCCTCAGT168110.26485836355942166No Hit
TATTGCACTTGTCCCGGCCTGTTT163430.25748499409027586No Hit
TGTAAACATCCTACACTCTCAGCT162740.2563978947454659No Hit
CAAAGAATTCTCCTTTTGGGCT162640.25624034411578334No Hit
CCCATAAAGTAGAAAGCACT160740.2532468821518139No Hit
TAGCTTATCAGACTGATGTTGACA155830.24551114623439815No Hit
CCCCCCACTGCTAAATTTGACTGGCT154320.2431321317261909No Hit
AACATTCAACGCTGTCGGTGAGTT153350.24160389061826962No Hit
TATTGCACTTGTCCCGGCCTGTAAT149090.2348922337937908No Hit
AACGGAATCCCAAAAGCAGCTG140810.22184704165607141No Hit
CTCCCACTGCTTCACTTGACTAG138750.2186014986846098No Hit
TATTGCACTTGTCCCGGCCTGTAAA135300.21316600196056001No Hit
ACCGGGTGCTGTAGGCTT127460.20081403259344408No Hit
TTCTCACTACTGCACTTGACTAG127220.20043591108220585No Hit
ATCCCGGACGAGCCCCCA123060.19388180488740958No Hit
ACCCCCCACTGCTAAATTTGACTG122850.19355094856507613No Hit
CAACACCAGTCGATGGGCTGT119260.18789488095947074No Hit
TGAGGTAGTAGTTTGTACAGTT115980.1827272203058814No Hit
TGAGAACTGAATTCCATGGGTT115640.18219154816496055No Hit
TCGTTTCCCGGGCGGCGCACCA110600.17425099642895744No Hit
ACCGGGTGCTGTAGGCTTT106860.16835860287882812No Hit
TAGCAGCACGTAAATATTGGC106140.16722423834511338No Hit
CAAAGAATTCTCCTTTTGGGCTT104450.16456163270347743No Hit
ACTGGACTTGGAGTCAGAAGGCA102930.16216686313230189No Hit
TCCTGTCTGAGCGTCGCT102240.16107976378749192No Hit
CGCGACCTCAGATCAGACGTGG99490.15674712147122039No Hit
CTTCTCACTACTGCACTTGACTAG99400.15660532590450604No Hit
ACTGGACTTGGAGTCAGAAGGCT93800.14778249064228036No Hit
TGAGGTAGTAGTTTGTGCTGTT92840.14627000459732736No Hit
CTCCCACTGCTTCACTTGACTAGCCT89990.14177981165137324No Hit
ATGCACCTGGGCAAGGATTCTGA89750.14140169014013498No Hit
TTCAAGTAATCCAGGATAGGCTT89690.1413071597623254No Hit
TGTAAACATCCCCGACTGGAAGC88760.13984193890627725No Hit
CCCCCCACTGCTAAATTTGACTGG87460.1377937807204034No Hit
CGCGACCTCAGATCAGACGTGGCG86310.13598194847905348No Hit
AGCAGCATTGTACAGGGCTATGT83400.13139722515528976No Hit
AACCGGGCGGAAACACCA83220.1311136340218611No Hit
TGATGTAGTAGGTTGTATAGTT80230.1264028701943513No Hit
ACTTGACTTGGAGTCAGAAGGC79900.12588295311639874No Hit
TGTCAGTTTGTCAAATACCCCAA77890.12271618545977843No Hit
GGAATACCGGGTGCTGTAGGCTTT76270.12016386525892028No Hit
CGACGGAATCCCAAAAGCAGCTG76200.12005357981814246No Hit
TATTGCACTTGTCCCGGCCTGA73770.11622509951685525No Hit
TTCACCACCTTCTCCACCCAGC72220.11378306475677491No Hit
ATCACATTGCCAGGGATTTCCA70370.11086837810764678No Hit
CCCCCCACTGCTAAATTTGACTGT68980.1086784243550586No Hit
TATTGCACTTGTCCCGGCCTGTTA68160.10738650919166128No Hit
TGAGAACTGAATTCCATAGGCTGT67940.10703989780635956No Hit
CAAAGTGCTGTTCGTGCAGGTAG67770.10677206173589915No Hit
TATTGCACTTGTCCCGGCCTGTATT67690.10664602123215305No Hit
TTTAAACATCCTTGACTGGAAGCT67160.10581100289483528No Hit
CAAGGGAATCCCAAAAGCAGCTG66890.10538561619469224No Hit
ATCACATTGCCAGGGATTTCC66890.10538561619469224No Hit
AGCTACATCTGGCTACTGGGTCTC66780.10521231050204138No Hit
TGTCAGTTTGTCAAATACCCCA66190.10428276178691402No Hit
GAATACCGGGTGCTGTAGGCTTT64630.10182497196386543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)