FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6299570
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4139256.570686570670696No Hit
AACATTCAACGCTGTCGGTGAGT3299545.237722574715417No Hit
AAGCTGCCAGTTGAAGAACTGT2613984.14945782013693No Hit
CCCCCCACTGCTAAATTTGACTG2263723.593451616538906No Hit
TAGCAGCACGTAAATATTGGCG1929253.062510615803936No Hit
TTCAAGTAATCCAGGATAGGCT1507532.393068098298773No Hit
AACATTCAACGCTGTCGGTGAG1319842.0951271277245906No Hit
TAGCTTATCAGACTGATGTTGAC1087931.7269908898543869No Hit
CCCCCACTGCTAAATTTGACTG993631.5772981330471763No Hit
TATTGCACTTGTCCCGGCCTGTAA849351.3482666277222097No Hit
TAGCTTATCAGACTGATGTTGACT815991.2953106323129993No Hit
CGCCTGTCTGAGCGTCGCT744141.1812552285314712No Hit
TATTGCACTTGTCCCGGCCTGTAT729471.1579679247948669No Hit
TATTGCACTTGTCCCGGCCTGT725631.1518722706470441No Hit
TAGCTTATCAGACTGATGTTGA643491.0214824186412723No Hit
TGTAAACATCCTTGACTGGAAGCT498500.7913238522629323No Hit
AGCAGCATTGTACAGGGCTATGA454810.7219699122321047No Hit
CATAAAGTAGAAAGCACTACT442710.7027622520267256No Hit
TATTGCACTTGTCCCGGCCTGTA405280.6433454981847967No Hit
TGAGGTAGTAGGTTGTATAGTT397850.6315510423727333No Hit
ACTGGACTTGGAGTCAGAAGGC370330.5878655209800034No Hit
TGTAAACATCCCCGACTGGAAGCT328470.5214165411289977No Hit
TCGTACGACTCTTAGCGG319630.5073838373095306No Hit
AACATTCAACGCTGTCGGTGAGTT280980.4460304433477206No Hit
CCCATAAAGTAGAAAGCACTAC276130.43833150516622565No Hit
CAACGGAATCCCAAAAGCAGCTGT264820.4203778988089663No Hit
TTCAAGTAATTCAGGATAGGTT248110.3938522788063312No Hit
TGAGGTAGTAGATTGTATAGTT236410.37527958257468363No Hit
CTACGCCTGTCTGAGCGTCGCT217730.3456267650014207No Hit
TTAATGCTAATCGTGATAGGGGTT213380.3387215317870902No Hit
ACCGGGTGCTGTAGGCTT207310.32908595348571407No Hit
TACGCCTGTCTGAGCGTCGCT207160.32884784199556477No Hit
CCCATAAAGTAGAAAGCACT201130.31927576009156183No Hit
TGTAAACATCCTACACTCTCAGCT199060.3159898215275011No Hit
ACGCCTGTCTGAGCGTCGCT197400.3133547210365152No Hit
CATTGCACTTGTCTCGGTCTGA197350.31327535053979877No Hit
CGCGACCTCAGATCAGACGTGG184200.29240090990337436No Hit
ACCGGGTGCTGTAGGCTTA159440.253096639929392No Hit
CAACACCAGTCGATGGGCTGT154380.24506434566168803No Hit
TGTCAGTTTGTCAAATACCCCAA152870.24266735666085149No Hit
ACCGGGTGCTGTAGGCTTT150800.23938141809679075No Hit
CAACGGAATCCCAAAAGCAGCT144580.22950772830526528No Hit
ACTTGACTTGGAGTCAGAAGGC144420.22925374271577265No Hit
TGATGTAGTAGGTTGTATAGTT139990.22222151670669585No Hit
GCGTTCGCGCTTTCCCCTG135250.2146971936179771No Hit
TTCTCACTACTGCACTTGACTAG130850.20771258990693017No Hit
CACCCGGCTGTGTGCACATGTGC129080.20490287432316812No Hit
CGCGACCTCAGATCAGAC125730.19958505104316643No Hit
ACCACAGGGTAGAACCACGGAC124270.1972674325390463No Hit
TGAGGTAGTAGTTTGTACAGTT119320.1894097533641185No Hit
CGCGACCTCAGATCAGACGTGGCG116790.1853936062302665No Hit
ATCCCGGACGAGCCCCCA116310.18463164946178864No Hit
GGAATACCGGGTGCTGTAGGCTTT115600.1835045884084152No Hit
GCCTGTCTGAGCGTCGCT113330.1799011678574887No Hit
TTCAAGTAATCCAGGATAGGCTT108360.1720117404838743No Hit
TATTGCACTTGTCCCGGCCTGTT105840.1680114674493656No Hit
TGTAAACATCCCCGACTGGAAGC101910.1617729464074532No Hit
AACATTCAACGCTGTCGGTGA101740.1615030867186173No Hit
TATTGCACTTGTCCCGGCCTGTAG100810.16002679547969148No Hit
GGAATACCGGGTGCTGTAGGCTT98340.1561058929418992No Hit
TAGCTTATCAGACTGATGTTGACA96360.1529628212719281No Hit
CTTCTCACTACTGCACTTGACTAG94550.15008960929079285No Hit
AACCGGGCGGAAACACCA93040.1476926202899563No Hit
CCTCGACACAAGGGTTTGT92830.14735926420374723No Hit
CACTAGATTGTGAGCTCCTGGA91520.14527975718977645No Hit
TTATAAAGCAATGAGACTGATT91340.14499402340159726No Hit
AGCTCGGTCTGAGGCCCCTCAGT90770.1440891997390298No Hit
TGAGGTAGTAGTTTGTGCTGTT90240.1432478724738355No Hit
ATCCCACCGCTGCCACCA89210.1416128402414768No Hit
TCGTTTCCCGGCCAATGCACCA88510.1405016532874466No Hit
CTCCCACTGCTTCACTTGACTAG88360.14026354179729728No Hit
CAAAGAATTCTCCTTTTGGGCTT87420.13877137645902815No Hit
TGTCAGTTTGTCAAATACCCCA86300.13699347733257985No Hit
CCCCCACTGCTAAATTTGACTGGCTT85990.1365013802529379No Hit
TGAGAACTGAATTCCATGGGTT83660.13280271510595168No Hit
ACTGGACTTGGAGTCAGAAGGCA83120.1319455137414141No Hit
AACATTCAACGCTGTCGGTGAGA82540.13102481597950338No Hit
TGATGTAGTAGATTGTATAGTT81810.1298660067274433No Hit
AGCAGCATTGTACAGGGCTATGT81510.12938978374714463No Hit
AACGGAATCCCAAAAGCAGCTG80300.12746901772660674No Hit
TAGCAGCACGTAAATATTGGC79380.12600860058702418No Hit
CCCCCCACTGCTAAATTTGACTGG79300.1258816077922779No Hit
TGTAAACATCCTACACTCAGCT78870.12519902152051648No Hit
TGCAAGTAATCCAGGATAGGCT78010.12383384897699368No Hit
TACCTGGTTGATCCTGCC77890.1236433597848742No Hit
CAAAGAATTCTCCTTTTGGGCT75430.11973833134642524No Hit
ACTGGACTTGGAGTCAGAAGG74270.11789693582260376No Hit
CCAAGTCCTTCTGATCGAGGCCC73800.11715085315346921No Hit
AGCAGCATTGTACAGGGCTATG73750.11707148265675275No Hit
CCCCCCACTGCTAAATTTGACTGGCT73750.11707148265675275No Hit
CAAAGTGCTGTTCGTGCAGGTAG73570.11678574886857358No Hit
CAAAGTGCTGTTCGTGCAGGT73010.11589679930534942No Hit
ACTGGACTTGGAGTCAGAAGGCT71340.11324582471502022No Hit
TAGCAGCACGTAAATATTGGCGT71290.11316645421830379No Hit
TACTCCTGTCTGAGCGTCGCT71090.11284897223143803No Hit
CACTAGATTGTGAGCTCCTGGAA70140.1113409327938256No Hit
ACCTGGTGCTGTAGGCTT69820.11083296161484037No Hit
CGCGACCTCAGATCAGACGTGGC68320.10845184671334711No Hit
TGAGGTAGTAGGTTGTGTGGTT68230.10830897981925752No Hit
GAATACCGGGTGCTGTAGGCTTT67160.10661045118952564No Hit
TTCACAGTGGCTAAGTTCCG66320.1052770268446894No Hit
TTCACAGTGGCTAAGTTCTGC64760.10280066734713639No Hit
TTCAAGTAATCCAGGATAGGC63710.10113388691609111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)