FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7494635
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7423939.905659181534524No Hit
CCCCCCACTGCTAAATTTGACTG4358585.815600092599573No Hit
AACATTCAACGCTGTCGGTGAGT2297003.064859062516053No Hit
TATTGCACTTGTCCCGGCCTGTAT2009402.681117892999459No Hit
TATTGCACTTGTCCCGGCCTGTAA1959982.615177390226475No Hit
AACATTCAACGCTGTCGGTGAG1825942.436329454336335No Hit
AAGCTGCCAGTTGAAGAACTGT1749742.334656724443552No Hit
TTCAAGTAATCCAGGATAGGCT1737592.318445127748049No Hit
TAGCAGCACGTAAATATTGGCG1723912.3001920707279275No Hit
CCCCCACTGCTAAATTTGACTG1587022.117541414625262No Hit
TAGCTTATCAGACTGATGTTGACT1276201.7028180825350399No Hit
TAGCTTATCAGACTGATGTTGAC1095051.4611118486757528No Hit
GGAATACCGGGTGCTGTAGGCTTT898771.1992178404952343No Hit
TATTGCACTTGTCCCGGCCTGT817871.0912739579712687No Hit
TATTGCACTTGTCCCGGCCTGTA778701.0390099050854378No Hit
TGAGGTAGTAGGTTGTATAGTT690350.9211255784971516No Hit
TAGCTTATCAGACTGATGTTGA624800.8336630136090684No Hit
GGAATACCGGGTGCTGTAGGCTT588370.7850549092784372No Hit
TGTAAACATCCTTGACTGGAAGCT582490.7772092970504901No Hit
AGCAGCATTGTACAGGGCTATGA536810.7162590306265749No Hit
TGTAAACATCCCCGACTGGAAGCT491700.6560693082451647No Hit
GCCTGTCTGAGCGTCGCT445040.5938114397832583No Hit
TGAATACCGGGTGCTGTAGGCTTT434430.579654646290313No Hit
TGAGGTAGTAGATTGTATAGTT431020.5751047249132213No Hit
ACTGGACTTGGAGTCAGAAGGC385650.5145680877054052No Hit
CAACGGAATCCCAAAAGCAGCTGT384240.5126867419160506No Hit
TTCAAGTAATTCAGGATAGGTT348390.4648525245058632No Hit
CCCCCACTGCTAAATTTGACTGGCTT313800.41869950971595016No Hit
TGAATACCGGGTGCTGTAGGCTT280510.37428106905806624No Hit
CATAAAGTAGAAAGCACTACT280030.3736406109170093No Hit
CCCATAAAGTAGAAAGCACTAC274480.36623531366103884No Hit
TATTGCACTTGTCCCGGCCTGTAG267530.3569620134936525No Hit
CACTAGATTGTGAGCTCCTGGAA257570.3436725070667217No Hit
CACCCGGCTGTGTGCACATGTGC244520.3262600513567372No Hit
AGCTCGGTCTGAGGCCCCTCAGT217700.29047445272518274No Hit
CATTGCACTTGTCTCGGTCTGA216080.2883129064991157No Hit
CACTAGATTGTGAGCTCCTGGA215100.28700530446112454No Hit
CAAAGAATTCTCCTTTTGGGCT198990.26550992810190227No Hit
TCCTGTCTGAGCGTCGCT193620.2583448026488281No Hit
TATTGCACTTGTCCCGGCCTGTT192350.25665025715061507No Hit
ACCGGGTGCTGTAGGCTTA188870.2520069356279525No Hit
TGATGTAGTAGGTTGTATAGTT188140.2510329055384285No Hit
TATTGCACTTGTCCCGGCCTGTTT186170.24840435858450746No Hit
TATTGCACTTGTCCCGGCCTGTAAA185810.24792401497871477No Hit
TCGTACGACTCTTAGCGG182480.2434808366251325No Hit
TAGCTTATCAGACTGATGTTGACA177050.2362356539044263No Hit
GGAATACCGGGTGCTGTAGGCTTA175470.234127479190114No Hit
TATTGCACTTGTCCCGGCCTGTAAT173430.23140553209062215No Hit
CAACGGAATCCCAAAAGCAGCT167850.22396020620083565No Hit
AACATTCAACGCTGTCGGTGAGTT167780.22386680605526485No Hit
TGTAAACATCCTACACTCTCAGCT162510.2168351093815776No Hit
CTCCCACTGCTTCACTTGACTAG161350.2152873355406901No Hit
TGAGGTAGTAGTTTGTACAGTT157830.21059064250627282No Hit
TTCTCACTACTGCACTTGACTAG156010.20816223872143205No Hit
ACCCCCCACTGCTAAATTTGACTG145680.19437904581076998No Hit
CCCATAAAGTAGAAAGCACT143420.19136355539662706No Hit
CCCCCCACTGCTAAATTTGACTGGCT141980.18944218097345636No Hit
CAACACCAGTCGATGGGCTGT139340.1859196611976434No Hit
AACGGAATCCCAAAAGCAGCTG136730.18243717005564647No Hit
ACTGGACTTGGAGTCAGAAGGCA129840.17324392715589218No Hit
TGTAAACATCCCCGACTGGAAGC128800.1718562678502689No Hit
CTTCTCACTACTGCACTTGACTAG128150.17098898078425434No Hit
ACCGGGTGCTGTAGGCTT126460.1687340344126165No Hit
CGCGACCTCAGATCAGACGTGGCG120180.16035470706712202No Hit
CGCGACCTCAGATCAGACGTGG119790.15983433482751327No Hit
ATCCCGGACGAGCCCCCA118680.15835327537631919No Hit
CAAAGAATTCTCCTTTTGGGCTT118400.15797967479403602No Hit
ACTGGACTTGGAGTCAGAAGGCT117640.1569656160706959No Hit
TGATGTAGTAGATTGTATAGTT116750.1557780999341529No Hit
ACTTGACTTGGAGTCAGAAGGC115380.153950125656553No Hit
TAGCAGCACGTAAATATTGGC109150.14563751270075193No Hit
TGAGGTAGTAGTTTGTGCTGTT107990.14408973885986442No Hit
ATGCACCTGGGCAAGGATTCTGA107910.14398299583635493No Hit
GGAATACCGGGTGCTGTAGGCT107600.14356936662025568No Hit
CGCCTGTCTGAGCGTCGCT102440.13668444160389398No Hit
AGCAGCATTGTACAGGGCTATGT101770.13579046878200207No Hit
ACCGGGTGCTGTAGGCTTT100740.13441615235431745No Hit
TCGTTTCCCGGGCGGCGCACCA99660.13297512153693944No Hit
TTCAAGTAATCCAGGATAGGCTT99450.13269492110022704No Hit
CTCCCACTGCTTCACTTGACTAGCCT96940.12934585873761698No Hit
CCCCCCACTGCTAAATTTGACTGG93280.12446236541205809No Hit
GAACGGAATCCCAAAAGCAGCTG88560.11816452702499854No Hit
TATTGCACTTGTCCCGGCCTGA87480.11672349620762053No Hit
TCGTTTCCCGGCCAATGCACCA84990.11340111960088783No Hit
TATTGCACTTGTCCCGGCCTGTTA84460.1126939470701375No Hit
TGAATACCGGGTGCTGTAGGCTTA83470.11137300215420763No Hit
GCCCCCACTGCTAAATTTGACTG82940.1106658296234573No Hit
TATTGCACTTGTCCCGGCCTGTATT81430.10865105505471581No Hit
CAAAGTGCTGTTCGTGCAGGTAG79880.10658290897421956No Hit
CAAGGGAATCCCAAAAGCAGCTG77980.10404776216586932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)