FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000040
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG110017511.001705993176026No Hit
CCCCCCACTGCTAAATTTGACTG100665610.066519733921064No Hit
TTCAAGTAATCCAGGATAGGCT3805333.8053147787408848No Hit
TAGCAGCACGTAAATATTGGCG3272933.272916908332367No Hit
GCCTGTCTGAGCGTCGCT3012053.0120379518481926No Hit
CCCCCACTGCTAAATTTGACTG2712432.7124191503233988No Hit
AAGCTGCCAGTTGAAGAACTGT2492172.492160031359875No Hit
AACATTCAACGCTGTCGGTGAGT2451422.4514101943592226No Hit
AACATTCAACGCTGTCGGTGAG2121362.1213515145939414No Hit
TATTGCACTTGTCCCGGCCTGTAA1486921.4869140523437907No Hit
TATTGCACTTGTCCCGGCCTGTAT1304911.3049047803808784No Hit
GGAATACCGGGTGCTGTAGGCTTT1186981.1869752520989916No Hit
TAGCTTATCAGACTGATGTTGAC1141381.141375434498262No Hit
TAGCTTATCAGACTGATGTTGACT1118811.118805524777901No Hit
TGAGGTAGTAGGTTGTATAGTT895030.8950264198943203No Hit
CAACGGAATCCCAAAAGCAGCTGT804980.8049767800928797No Hit
TAGCTTATCAGACTGATGTTGA778540.7785368858524566No Hit
TATTGCACTTGTCCCGGCCTGT760900.7608969564121744No Hit
TGTAAACATCCTTGACTGGAAGCT751250.75124699501202No Hit
AACCGGGCGGAAACACCA667370.6673673305306779No Hit
GGAATACCGGGTGCTGTAGGCTT656220.6562173751304995No Hit
CATAAAGTAGAAAGCACTACT581780.5817776728893084No Hit
TATTGCACTTGTCCCGGCCTGTA580140.5801376794492822No Hit
CCCATAAAGTAGAAAGCACTAC539330.5393278426886292No Hit
TGAGGTAGTAGATTGTATAGTT509410.5094079623681506No Hit
AGCAGCATTGTACAGGGCTATGA506620.5066179735281059No Hit
TTCAAGTAATTCAGGATAGGTT495460.49545801816792734No Hit
TGTAAACATCCCCGACTGGAAGCT425920.4259182963268147No Hit
CCCCCACTGCTAAATTTGACTGGCTT421930.4219283122867509No Hit
ACTGGACTTGGAGTCAGAAGGC382930.38292846828612687No Hit
CTCCCACTGCTTCACTTGACTAG354450.35444858220567116No Hit
GACCCGCGGGCGCTCTCCAGTCCT326840.3268386926452294No Hit
TTCTCACTACTGCACTTGACTAG324700.3246987012051952No Hit
TCGTACGACTCTTAGCGG315950.31594873620505515No Hit
TTAATGCTAATCGTGATAGGGGTT299340.2993388026447894No Hit
CATTGCACTTGTCTCGGTCTGA281250.28124887500449997No Hit
TGTAAACATCCTACACTCTCAGCT278970.2789688841244635No Hit
CAACGGAATCCCAAAAGCAGCT262710.26270894916420334No Hit
CCCATAAAGTAGAAAGCACT259420.25941896232415074No Hit
CTACGCCTGTCTGAGCGTCGCT259310.25930896276414894No Hit
TAGCAGCACGTAAATATTGGC252910.25290898836404657No Hit
CTTCTCACTACTGCACTTGACTAG252470.2524689901240395No Hit
CACCCGGCTGTGTGCACATGTGC250540.2505389978440086No Hit
AACGGAATCCCAAAAGCAGCTG250200.2501989992040032No Hit
CCCCCCACTGCTAAATTTGACTGGCT237820.23781904872380513No Hit
GGGAATACCGGGTGCTGTAGGCTTT229970.22996908012367948No Hit
ACCCCCCACTGCTAAATTTGACTG220520.2205191179235283No Hit
CCCCCCACTGCTAAATTTGACTGG218270.21826912692349232No Hit
TGAGAACTGAATTCCATGGGTT215030.21502913988344047No Hit
AGCTCGGTCTGAGGCCCCTCAGT214070.21406914372342511No Hit
CAAAGAATTCTCCTTTTGGGCT205530.20552917788328848No Hit
TAGCTTATCAGACTGATGTTGACA200800.2007991968032128No Hit
TGAGGTAGTAGTTTGTACAGTT191110.19110923556305773No Hit
GGAATACCGGGTGCTGTAGGCTTA190870.1908692365230539No Hit
TTCAAGTAATCCAGGATAGGC179000.178999284002864No Hit
GGGAATACCGGGTGCTGTAGGCTT172030.17202931188275247No Hit
TATTGCACTTGTCCCGGCCTGTAG165150.1651493394026424No Hit
AACATTCAACGCTGTCGGTGAGTT164190.16418934324262702No Hit
CTCCCACTGCTTCACTTGACTAGCCT162000.16199935200259197No Hit
GAATACCGGGTGCTGTAGGCTTT158130.1581293674825301No Hit
TTCAAGTAATCCAGGATAGGCTT151700.1516993932024272No Hit
CGCCTGTCTGAGCGTCGCT150480.15047939808240765No Hit
CACTAGATTGTGAGCTCCTGGAA145930.14592941628233486No Hit
TCGTTTCCCGGCCAATGCACCA145800.1457994168023328No Hit
TCGTTTCCCGGGCGGCGCACCA145780.14577941688233248No Hit
ATCACATTGCCAGGGATTTCC144190.14418942324230705No Hit
CACTAGATTGTGAGCTCCTGGA143200.14319942720229117No Hit
TATTGCACTTGTCCCGGCCTGTT143140.14313942744229022No Hit
CAACACCAGTCGATGGGCTGT138450.1384494462022152No Hit
TGAGGTAGTAGTTTGTGCTGTT138170.1381694473222107No Hit
TGTAAACATCCCCGACTGGAAGC134410.13440946236215054No Hit
TGTCAGTTTGTCAAATACCCCA131970.13196947212211152No Hit
ACCGGGTGCTGTAGGCTTT129290.12928948284206865No Hit
TGTCAGTTTGTCAAATACCCCAA127260.12725949096203615No Hit
ACCGGGTGCTGTAGGCTT126840.12683949264202943No Hit
TCCTGTCTGAGCGTCGCT126260.12625949496202016No Hit
ACCGGGTGCTGTAGGCTTA124640.12463950144199425No Hit
TTCACAGTGGCTAAGTTCTGC123720.1237195051219795No Hit
TATTGCACTTGTCCCGGCCTGTAAT123010.12300950796196816No Hit
TATTGCACTTGTCCCGGCCTGTAAA121070.12106951572193711No Hit
CAAAGAATTCTCCTTTTGGGCTT120140.12013951944192224No Hit
TGAGAACTGAATTCCATAGGCTGT118400.11839952640189438No Hit
TTCACAGTGGCTAAGTTCCGC117680.11767952928188287No Hit
TGAGGTAGTAGGTTGTGTGGTT115550.11554953780184879No Hit
CCCATAAAGTAGAAAGCACTA115230.11522953908184366No Hit
ATCCCGGACGAGCCCCCA113930.11392954428182288No Hit
TACGCCTGTCTGAGCGTCGCT112920.1129195483218067No Hit
GGGCTACGCCTGTCTGAGCGTCGCT112410.11240955036179855No Hit
ATGCACCTGGGCAAGGATTCTGA108840.10883956464174144No Hit
GACCCGCGGGCGCTCTCCAGTCCTT105680.10567957728169086No Hit
GAATACCGGGTGCTGTAGGCTT105070.10506957972168111No Hit
TAGCAGCACGTAAATATTGGCGT104480.10447958208167167No Hit
AGCAGCATTGTACAGGGCTATGT103730.10372958508165968No Hit
ACTGGACTTGGAGTCAGAAGGCT103590.10358958564165743No Hit
TGAATACCGGGTGCTGTAGGCTTT101770.10176959292162832No Hit
ACTGGACTTGGAGTCAGAAGGCA100020.10001959992160031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)