FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10222751
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8423918.240355262492455No Hit
CCCCCCACTGCTAAATTTGACTG8171317.99325934868217No Hit
GCCTGTCTGAGCGTCGCT4254374.161668419782503No Hit
AACATTCAACGCTGTCGGTGAGT4027463.93970272776868No Hit
TTCAAGTAATCCAGGATAGGCT3769553.6874125174329295No Hit
TAGCAGCACGTAAATATTGGCG3178063.1088109257478735No Hit
AACATTCAACGCTGTCGGTGAG2884542.821686647752645No Hit
AAGCTGCCAGTTGAAGAACTGT2879142.816404312303019No Hit
CCCCCACTGCTAAATTTGACTG2787522.726780687507697No Hit
TAGCTTATCAGACTGATGTTGAC1418051.3871510711744812No Hit
TATTGCACTTGTCCCGGCCTGTAA1401711.3711671153880203No Hit
TAGCTTATCAGACTGATGTTGACT1328591.29964038055901No Hit
TATTGCACTTGTCCCGGCCTGTAT1219491.192917640271195No Hit
TAGCTTATCAGACTGATGTTGA1177791.1521262720768608No Hit
TATTGCACTTGTCCCGGCCTGT1102991.0789561439968556No Hit
TGAGGTAGTAGGTTGTATAGTT1068291.0450122476816661No Hit
CATAAAGTAGAAAGCACTACT930680.9104007326403627No Hit
AACCGGGCGGAAACACCA822860.8049301014961627No Hit
TTCAAGTAATTCAGGATAGGTT732520.7165585858444562No Hit
TGTAAACATCCTTGACTGGAAGCT708380.6929445899640909No Hit
TGAGGTAGTAGATTGTATAGTT678000.6632265620086022No Hit
TATTGCACTTGTCCCGGCCTGTA619660.6061577749472721No Hit
CAACGGAATCCCAAAAGCAGCT586200.5734268593649595No Hit
CAACGGAATCCCAAAAGCAGCTGT573430.5609351142368625No Hit
TCGTACGACTCTTAGCGG549350.5373798109726041No Hit
AGCAGCATTGTACAGGGCTATGA506400.4953656799427082No Hit
CCCATAAAGTAGAAAGCACTAC485300.4747254432784287No Hit
CCCATAAAGTAGAAAGCACT478340.4679170998100218No Hit
ACCGGGTGCTGTAGGCTT429650.420288041839227No Hit
TGTAAACATCCCCGACTGGAAGCT418000.4088918922117931No Hit
TTAATGCTAATCGTGATAGGGGTT407090.39821961818301166No Hit
CGCCTGTCTGAGCGTCGCT404160.3953534620964552No Hit
CATTGCACTTGTCTCGGTCTGA403360.3945708938816958No Hit
ACTGGACTTGGAGTCAGAAGGC393240.3846714059649893No Hit
ACCGGGTGCTGTAGGCTTT357530.349739517278666No Hit
CTCCCACTGCTTCACTTGACTAG309680.30293215593336864No Hit
ACCGGGTGCTGTAGGCTTA309560.3028147707011547No Hit
CACCCGGCTGTGTGCACATGTGC296620.2901567298274212No Hit
CAACACCAGTCGATGGGCTGT285160.2789464401509926No Hit
TTCTCACTACTGCACTTGACTAG283530.27735195741342034No Hit
TGAGAACTGAATTCCATGGGTT279220.27313587115640403No Hit
TGTAAACATCCTACACTCTCAGCT274000.26802961355509886No Hit
TGAGGTAGTAGTTTGTACAGTT273410.26745246949671375No Hit
TCGTTTCCCGGCCAATGCACCA266730.26091802490347266No Hit
GACCCGCGGGCGCTCTCCAGTCCT266700.2608886785954192No Hit
TCCTGTCTGAGCGTCGCT266470.26066369023367586No Hit
AACGGAATCCCAAAAGCAGCTG248420.2430069948881666No Hit
AGCTCGGTCTGAGGCCCCTCAGT234270.22916531958960948No Hit
AACATTCAACGCTGTCGGTGAGTT228670.2236873420862936No Hit
TAGCAGCACGTAAATATTGGC200820.19644418610998154No Hit
ATCCCGGACGAGCCCCCA195660.19139662112478334No Hit
GGAATACCGGGTGCTGTAGGCTTT195070.19081947706639826No Hit
TAGCTTATCAGACTGATGTTGACA194190.18995865203016293No Hit
CAACGGAATCCCAAAAGCAGCTGA193090.18888262073486872No Hit
TTCAAGTAATCCAGGATAGGC192320.1881293988281628No Hit
TGAGGTAGTAGTTTGTGCTGTT178020.1741409919893383No Hit
CTTCTCACTACTGCACTTGACTAG177670.17379861839538105No Hit
CACCCGTAGAACCGACCTTGCG172780.16901517018266415No Hit
TTCAAGTAATCCAGGATAGGCTT171470.1677337147309956No Hit
CCCCCACTGCTAAATTTGACTGGCTT167770.16411433673773332No Hit
CACTAGATTGTGAGCTCCTGGA166870.16323394749612896No Hit
TATTGCACTTGTCCCGGCCTGTT165640.16203074886593638No Hit
CTACGCCTGTCTGAGCGTCGCT163630.16006454622635335No Hit
TGAGGTAGTAGGTTGTGTGGTT159210.1557408568398076No Hit
TTCACAGTGGCTAAGTTCTGC152760.14943140060830984No Hit
CCCCCCACTGCTAAATTTGACTGG149890.14662393713786043No Hit
TATTGCACTTGTCCCGGCCTGTAG149450.14619352461974275No Hit
GGAATACCGGGTGCTGTAGGCTT143280.1401579672634108No Hit
AATGCACCCGGGCAAGGATTCT139370.1363331651137742No Hit
CAAAGAATTCTCCTTTTGGGCT139330.1362940367030362No Hit
CACTAGATTGTGAGCTCCTGGAA136540.13356483005406275No Hit
ACCCCCCACTGCTAAATTTGACTG134750.13181383367353855No Hit
TGAGAACTGAATTCCATAGGCTGT134730.13179426946816958No Hit
CCCATAAAGTAGAAAGCACTA129870.12704016756350614No Hit
AACATTCAACGCTGTCGGTGA129510.1266880118668644No Hit
CAAAGTGCTGTTCGTGCAGGT124690.12197303837293895No Hit
TCGTTTCCCGGGCGGCGCACCA124560.12184587103804055No Hit
TACGCCTGTCTGAGCGTCGCT122060.11940034536691738No Hit
CCCCCCACTGCTAAATTTGACTGGCT120370.11774717001323812No Hit
TGTAAACATCCCCGACTGGAAGC120070.11745370693270335No Hit
TGTCAGTTTGTCAAATACCCCA118420.11583965998976205No Hit
TGATGTAGTAGGTTGTATAGTT117670.11510600228842512No Hit
TATTGCACTTGTCCCGGCCTGA115960.11343326272937686No Hit
TTATAAAGCAATGAGACTGATT114340.11184856209448905No Hit
AGCAGCATTGTACAGGGCTATGT112960.11049863192402905No Hit
ACCACAGGGTAGAACCACGGAC112620.11016604043275631No Hit
TTCACAGTGGCTAAGTTCCGC112420.10997039837906647No Hit
TGTCAGTTTGTCAAATACCCCAA111230.10880632815961185No Hit
ATCACATTGCCAGGGATTTCC110950.10853242928444604No Hit
AATGACACGATCACTCCCGTTG110770.10835635143612518No Hit
TTCACAGTGGCTAAGTTCCG110180.1077792073777401No Hit
TGAGGTAGTAGGTTGTATAGT108730.10636080248848867No Hit
TTCACAGTGGCTAAGTTCTG104800.10251643613348306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)