FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10211129
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6596336.459941892811265No Hit
CAACGGAATCCCAAAAGCAGCTG5697625.579813946136612No Hit
GCCTGTCTGAGCGTCGCT5159085.052408994147465No Hit
AACATTCAACGCTGTCGGTGAGT3802703.7240739980858137No Hit
TTCAAGTAATCCAGGATAGGCT3162363.0969738997519274No Hit
AAGCTGCCAGTTGAAGAACTGT2975482.9139578982892096No Hit
TAGCAGCACGTAAATATTGGCG2943842.882972098384028No Hit
AACATTCAACGCTGTCGGTGAG2020841.9790563805432289No Hit
CCCCCACTGCTAAATTTGACTG1951181.9108366959226548No Hit
TAGCTTATCAGACTGATGTTGAC1616491.5830668675324737No Hit
TAGCTTATCAGACTGATGTTGACT1265391.239226338243303No Hit
TATTGCACTTGTCCCGGCCTGTAA1255581.2296191733548758No Hit
AACCGGGCGGAAACACCA1183091.1586280028388634No Hit
TATTGCACTTGTCCCGGCCTGTAT1112571.08956609988964No Hit
TAGCTTATCAGACTGATGTTGA1087301.064818591558289No Hit
CGCCTGTCTGAGCGTCGCT1025311.0041103192408989No Hit
TATTGCACTTGTCCCGGCCTGT1009420.9885488666336505No Hit
TGAGGTAGTAGGTTGTATAGTT943880.924363995401488No Hit
TGTAAACATCCTTGACTGGAAGCT856990.8392705644987934No Hit
AGCAGCATTGTACAGGGCTATGA740040.7247386650389002No Hit
CATAAAGTAGAAAGCACTACT707460.6928323009140321No Hit
TATTGCACTTGTCCCGGCCTGTA642760.6294700615377594No Hit
TCGTACGACTCTTAGCGG613960.6012655407643954No Hit
TGAGGTAGTAGATTGTATAGTT613750.6010598828004229No Hit
ACTGGACTTGGAGTCAGAAGGC537160.526053485368758No Hit
TGTAAACATCCCCGACTGGAAGCT536860.5257596882773687No Hit
TTCAAGTAATTCAGGATAGGTT499600.489270089526829No Hit
TCCTGTCTGAGCGTCGCT427180.41834747166547404No Hit
CAACGGAATCCCAAAAGCAGCTGT377410.36960653420400424No Hit
CTACGCCTGTCTGAGCGTCGCT375910.36813754874705823No Hit
TACGCCTGTCTGAGCGTCGCT373680.3659536570343984No Hit
CCCATAAAGTAGAAAGCACTAC364610.3570711916380647No Hit
CCCATAAAGTAGAAAGCACT345770.3386207342988224No Hit
TGTAAACATCCTACACTCTCAGCT341840.33477199240162375No Hit
CATTGCACTTGTCTCGGTCTGA329210.3224031348541381No Hit
AACATTCAACGCTGTCGGTGAGTT327700.3209243561608124No Hit
GCGTTCGCGCTTTCCCCTG322830.3161550500439275No Hit
TTCTCACTACTGCACTTGACTAG319780.31316811294813723No Hit
CTCCCACTGCTTCACTTGACTAG304750.2984488786695379No Hit
ACCGGGTGCTGTAGGCTT298430.2922595532776052No Hit
ACGCCTGTCTGAGCGTCGCT294440.28835205196212876No Hit
GACCCGCGGGCGCTCTCCAGTCCT293010.2869516191598402No Hit
CTTCTCACTACTGCACTTGACTAG285000.2791072368197483No Hit
TGAGGTAGTAGTTTGTACAGTT250340.24516387952791507No Hit
ACCGGGTGCTGTAGGCTTT236430.23154148772383545No Hit
ATCCCGGACGAGCCCCCA230690.22592017004192189No Hit
CAACACCAGTCGATGGGCTGT227740.22303116530992803No Hit
ACCGGGTGCTGTAGGCTTA226570.2218853566535101No Hit
AGCTCGGTCTGAGGCCCCTCAGT225020.22036740501466587No Hit
TTCACAGTGGCTAAGTTCCG203570.19936091298033742No Hit
TAGCTTATCAGACTGATGTTGACA201180.19702032948560338No Hit
GGAATACCGGGTGCTGTAGGCTTT192210.18823579645306607No Hit
CAACGGAATCCCAAAAGCAGCT189800.18587562648557274No Hit
CCCCCACTGCTAAATTTGACTGGCTT182990.1792064325110377No Hit
TTCAAGTAATCCAGGATAGGCTT182750.17897139483792635No Hit
TGAGGTAGTAGTTTGTGCTGTT178990.1752891379591816No Hit
TGTCAGTTTGTCAAATACCCCAA176140.1724980655909841No Hit
TGTAAACATCCTACACTCAGCT175160.17153832842577935No Hit
ATCCCACCGCTGCCACCA173080.1695013352588142No Hit
TGTAAACATCCCCGACTGGAAGC169860.16634791314457No Hit
TATTGCACTTGTCCCGGCCTGTT166820.16337076928515937No Hit
CCCCCCACTGCTAAATTTGACTGGCT163710.16032507277109123No Hit
CACCCGGCTGTGTGCACATGTGC163390.16001168920694275No Hit
TAGCAGCACGTAAATATTGGC161010.15768089894858836No Hit
TTCAAGTAATCCAGGATAGGC159640.15633922556457763No Hit
AACGGAATCCCAAAAGCAGCTG157750.15448830388882562No Hit
AGCAGCATTGTACAGGGCTATGT156770.15352856672362086No Hit
CCCCCCACTGCTAAATTTGACTGG154110.15092356584663655No Hit
ACCACAGGGTAGAACCACGGAC148340.1452728684555841No Hit
TGTCAGTTTGTCAAATACCCCA147340.14429354481762005No Hit
TTATAAAGCAATGAGACTGATT144660.14166895746787647No Hit
GGAATACCGGGTGCTGTAGGCTT144030.14105198357595913No Hit
GGGCTACGCCTGTCTGAGCGTCGCT143330.14036645702938433No Hit
CAAAGAATTCTCCTTTTGGGCT142430.1394850657552167No Hit
TATTGCACTTGTCCCGGCCTGTAG136580.13375602247312712No Hit
ACTGGACTTGGAGTCAGAAGGCA136180.1333642930179415No Hit
AGCAGCATTGTACAGGGCTATG136050.1332369809450062No Hit
CACTAGATTGTGAGCTCCTGGA131680.12895733664710338No Hit
TAGCAGCACGTAAATATTGGCGT131350.12863415984657525No Hit
TGAGGTAGTAGGTTGTGTGGTT130470.1277723550451669No Hit
ACTGGACTTGGAGTCAGAAGG127820.12517714740456223No Hit
TCGTTTCCCGGCCAATGCACCA127150.12452100056712632No Hit
CAAAGTGCTGTTCGTGCAGGT126290.12367878223847725No Hit
CCTCGACACAAGGGTTTGT125250.12266028565499466No Hit
CCAAGTCCTTCTGATCGAGGCCC123300.12075060456096481No Hit
CACTAGATTGTGAGCTCCTGGAA119040.11657868586323805No Hit
AACATTCAACGCTGTCGGTGA118230.11578543371648718No Hit
TGATGTAGTAGGTTGTATAGTT112110.10979197305214732No Hit
TTCACAGTGGCTAAGTTCTGC111070.10877347646866473No Hit
ACTGGACTTGGAGTCAGAAGGCT110380.10809774315846954No Hit
CAAAGAATTCTCCTTTTGGGCTT109930.10765704752138575No Hit
CTCGACACAAGGGTTTGT109630.10736325042999652No Hit
TATTGCACTTGTCCCGGCCTGA108150.10591385144580977No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT107730.10550253551786487No Hit
AACATTCAACGCTGTCGGTGAGA107300.10508142635354033No Hit
TTCACAGTGGCTAAGTTCTG106780.10457217806179905No Hit
TACCTGGTTGATCCTGCC106650.10444486598886371No Hit
ACCCCCCACTGCTAAATTTGACTG106320.1041216891883356No Hit
TGAGGTAGTAGGTTGTATAGT104920.10275063609518593No Hit
GAATACCGGGTGCTGTAGGCTTT102510.10039046612769263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)