FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12485013
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9457717.575250422246256No Hit
CAACGGAATCCCAAAAGCAGCTG7369935.903021486641624No Hit
AAGCTGCCAGTTGAAGAACTGT3990323.1960879816464747No Hit
AACATTCAACGCTGTCGGTGAGT3806643.0489675901819244No Hit
TTCAAGTAATCCAGGATAGGCT3497912.8016871107783388No Hit
TAGCTTATCAGACTGATGTTGAC3213562.573934043961348No Hit
TAGCAGCACGTAAATATTGGCG3151192.523978148841335No Hit
CCCCCACTGCTAAATTTGACTG2674502.1421683741939237No Hit
TAGCTTATCAGACTGATGTTGACT2499302.001840126237754No Hit
AACATTCAACGCTGTCGGTGAG2145651.7185805092874151No Hit
TATTGCACTTGTCCCGGCCTGTAA2099031.6812397391977085No Hit
TGTAAACATCCTTGACTGGAAGCT1954221.5652526753476348No Hit
TATTGCACTTGTCCCGGCCTGTAT1788601.4325976272511691No Hit
TGAGGTAGTAGGTTGTATAGTT1518621.2163543602237339No Hit
TAGCTTATCAGACTGATGTTGA1230010.9851892024461649No Hit
CGCCTGTCTGAGCGTCGCT1176930.9426742286932341No Hit
TGAGGTAGTAGATTGTATAGTT1057490.8470075281459458No Hit
AGCAGCATTGTACAGGGCTATGA1046860.8384933199508884No Hit
TGTAAACATCCCCGACTGGAAGCT981550.7861826014918847No Hit
TATTGCACTTGTCCCGGCCTGT952780.7631389731031918No Hit
ACTGGACTTGGAGTCAGAAGGC865630.69333528126883No Hit
TATTGCACTTGTCCCGGCCTGTA779670.6244847322145359No Hit
TTCAAGTAATTCAGGATAGGTT666840.5341123793783795No Hit
CATAAAGTAGAAAGCACTACT616040.4934235951536454No Hit
TGTCAGTTTGTCAAATACCCCAA488640.39138125046405636No Hit
TTCTCACTACTGCACTTGACTAG474330.3799195082936638No Hit
TACGCCTGTCTGAGCGTCGCT460650.36896237112448343No Hit
CAACGGAATCCCAAAAGCAGCTGT451000.3612331040424227No Hit
TAGCTTATCAGACTGATGTTGACA448520.35924672245034905No Hit
TGAGGTAGTAGTTTGTACAGTT440120.35251865576751906No Hit
CCCCCACTGCTAAATTTGACTGGCTT435460.3487861806791871No Hit
CTCCCACTGCTTCACTTGACTAG429190.3437641594766461No Hit
CTTCTCACTACTGCACTTGACTAG395210.3165475278239598No Hit
CCCATAAAGTAGAAAGCACTAC377750.3025627606475059No Hit
CTACGCCTGTCTGAGCGTCGCT371260.2973645281747003No Hit
CGCGACCTCAGATCAGACGTGGCG361500.28954715545750737No Hit
AACATTCAACGCTGTCGGTGAGTT361200.289306867361692No Hit
TGTAAACATCCTACACTCTCAGCT348950.27949510344923145No Hit
GCCTGTCTGAGCGTCGCT334470.26789719802454354No Hit
CATTGCACTTGTCTCGGTCTGA327200.262074216502618No Hit
CTCCCACTGCTTCACTTGACTAGCCT323260.25891843284424293No Hit
GCGTTCGCGCTTTCCCCTG313930.2514454730643853No Hit
TTCAAGTAATCCAGGATAGGCTT281560.22551838752590805No Hit
TGAGGTAGTAGTTTGTGCTGTT277020.22188202767590232No Hit
CAACACCAGTCGATGGGCTGT276770.22168178759605617No Hit
CCCCCCACTGCTAAATTTGACTGGCT272830.21852600393768112No Hit
CAAAGAATTCTCCTTTTGGGCTT263430.21099697693546657No Hit
TGTCAGTTTGTCAAATACCCCA262800.2104923719342543No Hit
CAAAGAATTCTCCTTTTGGGCT259800.20808949097610072No Hit
CCCCCCACTGCTAAATTTGACTGG256560.20549437954129482No Hit
GGAATACCGGGTGCTGTAGGCTTT253840.2033157674725689No Hit
ACGCCTGTCTGAGCGTCGCT247710.19840588071474174No Hit
TTATAAAGCAATGAGACTGATT242970.19460932880085907No Hit
CACCCGGCTGTGTGCACATGTGC240670.19276712006627467No Hit
ACTGGACTTGGAGTCAGAAGGCA236120.18912275061307504No Hit
TATTGCACTTGTCCCGGCCTGTAG235250.1884259151352105No Hit
TGTAAACATCCCCGACTGGAAGC235190.18837785751604744No Hit
TAGCAGCACGTAAATATTGGC226290.18124931067352515No Hit
ACTGGACTTGGAGTCAGAAGGCT209970.1681776382611696No Hit
ACCCCCCACTGCTAAATTTGACTG206600.16547840198484373No Hit
CCAAGTCCTTCTGATCGAGGCCC197910.15851805680939218No Hit
CCCATAAAGTAGAAAGCACT192860.15447320719650032No Hit
AGCAGCATTGTACAGGGCTATGT188040.15061257845706688No Hit
TATTGCACTTGTCCCGGCCTGTAAT187010.14978758932810082No Hit
TATTGCACTTGTCCCGGCCTGTT185770.148794398532064No Hit
AGCTCGGTCTGAGGCCCCTCAGT179750.14397261740936915No Hit
GGAATACCGGGTGCTGTAGGCTT178620.1430675322484646No Hit
AACGGAATCCCAAAAGCAGCTG178300.14281122494626158No Hit
CAACGGAATCCCAAAAGCAGCT176460.14133745795859404No Hit
TAGCAGCACGTAAATATTGGCGT176220.14114522748194175No Hit
ACTGGACTTGGAGTCAGAAGG175870.14086489137015717No Hit
AACCGGGCGGAAACACCA167230.13394459421067484No Hit
CGCGACCTCAGATCAGACGTGG166260.13316766270087182No Hit
TGTAAACATCCTACACTCAGCT161290.12918688991353072No Hit
TGAGGTAGTAGGTTGTGTGGTT153690.12309959148620832No Hit
TTCACAGTGGCTAAGTTCCGC149160.1194712412393964No Hit
TTCACAGTGGCTAAGTTCCG148170.1186782905232057No Hit
ACCACAGGGTAGAACCACGGAC147780.11836591599864574No Hit
ATGCACCTGGGCAAGGATTCTGA147510.11814965671241191No Hit
CCTCGACACAAGGGTTTGT146850.11762102290161812No Hit
TTCACAGTGGCTAAGTTCTGC146630.11744481163135352No Hit
CAAGTCCTTCTGATCGAGGCCC141810.1135841828919201No Hit
TGTAAACATCCTTGACTGGAAGC139900.11205434868189565No Hit
TATTGCACTTGTCCCGGCCTGTAAA139390.11164585891900955No Hit
ATCACATTGCCAGGGATTTCCA137120.10982767899400665No Hit
CAAAGTGCTGTTCGTGCAGGTAG136260.10913885311933597No Hit
CACTAGATTGTGAGCTCCTGGAA134670.10786532621151455No Hit
TTCAAGTAATCCAGGATAGGC134440.10768110533805612No Hit
CTGCGTTCGCGCTTTCCCCTG133560.10697626025699773No Hit
ACCGGGTGCTGTAGGCTT132330.10599107906415475No Hit
GGGCTACGCCTGTCTGAGCGTCGCT131760.10553453168210558No Hit
TATTGCACTTGTCCCGGCCTGTTT129760.10393261104333652No Hit
TCCCACTGCTTCACTTGACTAG129030.10334791001018581No Hit
ACCGGGTGCTGTAGGCTTA125190.10027222238374921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)