FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7721449
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG119953215.535063431747073No Hit
TCGTACGACTCTTAGCGG4437885.747470455351062No Hit
CCCCCCACTGCTAAATTTGACTG3298624.2720220000158005No Hit
TTCAAGTAATCCAGGATAGGCT2333133.021621977947403No Hit
GCCTGTCTGAGCGTCGCT1883532.439347847793853No Hit
TAGCAGCACGTAAATATTGGCG1578412.0441888562626005No Hit
TGAGGTAGTAGATTGTATAGTT1496021.9374860858369976No Hit
CCCCCACTGCTAAATTTGACTG1476991.9128404526145288No Hit
AACATTCAACGCTGTCGGTGAG1442591.8682892291330293No Hit
AAGCTGCCAGTTGAAGAACTGT1381211.7887963774674935No Hit
TATTGCACTTGTCCCGGCCTGT1028251.3316801030480159No Hit
TAGCTTATCAGACTGATGTTGAC880101.1398119705252214No Hit
TGAGGTAGTAGGTTGTATAGTT848681.0991201262871775No Hit
TATTGCACTTGTCCCGGCCTGTAA758270.9820307043405971No Hit
TAGCTTATCAGACTGATGTTGA752340.9743507986648621No Hit
TATTGCACTTGTCCCGGCCTGTAT719980.932441566343312No Hit
TGAGAACTGAATTCCATGGGTT628060.8133965529008867No Hit
TTCAAGTAATTCAGGATAGGTT512020.6631138792731779No Hit
TAGCTTATCAGACTGATGTTGACT508190.6581536703797435No Hit
CAACGGAATCCCAAAAGCAGCTGT487070.6308012913120322No Hit
AACATTCAACGCTGTCGGTGAGT467590.6055728659219274No Hit
TATTGCACTTGTCCCGGCCTGTA443370.5742056963660578No Hit
CATAAAGTAGAAAGCACTACT434720.56300313581039No Hit
CATTGCACTTGTCTCGGTCTGA416670.5396266944196614No Hit
CAACGGAATCCCAAAAGCAGCT406850.5269088742281404No Hit
TGAGGTAGTAGTTTGTACAGTT400630.5188533913777065No Hit
CTCCCACTGCTTCACTTGACTAG381540.49413005253288605No Hit
TTCTCACTACTGCACTTGACTAG357220.4626333736064306No Hit
AGCAGCATTGTACAGGGCTATGA334750.43353261803581167No Hit
CACCCGGCTGTGTGCACATGTGC332630.4307870193793937No Hit
TGTAAACATCCTTGACTGGAAGCT318120.4119952097074007No Hit
AACGGAATCCCAAAAGCAGCTG299270.38758269335198614No Hit
ACTGGACTTGGAGTCAGAAGGC287940.3729092816646202No Hit
CCCATAAAGTAGAAAGCACT252520.32703706260314613No Hit
ACCGGGTGCTGTAGGCTT248880.3223229215138247No Hit
TTAATGCTAATCGTGATAGGGGTT244370.31648204890040715No Hit
TCGTTTCCCGGCCAATGCACCA239890.3106800290981654No Hit
CCCATAAAGTAGAAAGCACTAC230630.29868746138192453No Hit
TTCACAGTGGCTAAGTTCCGC227420.29453021058612183No Hit
CCCCCACTGCTAAATTTGACTGGCTT209820.27173656136302915No Hit
TGATGTAGTAGATTGTATAGTT207370.2685635817836782No Hit
TGTAAACATCCCCGACTGGAAGCT196810.25488739224982254No Hit
CTTCTCACTACTGCACTTGACTAG196480.25446001132688956No Hit
CAACACCAGTCGATGGGCTGT190620.24687076221056437No Hit
TGAGGTAGTAGATTGTATAGT187910.24336105826769042No Hit
CTCCCACTGCTTCACTTGACTAGCCT174770.22634352697272234No Hit
TCCCACTGCTTCACTTGACTAG171820.2225230005404426No Hit
TGAGAACTGAATTCCATAGGCTGT160150.20740925699308513No Hit
ACCGGGTGCTGTAGGCTTT158350.20507808832254154No Hit
TACCACCCTGAACGCGCCCGA153150.19834360105208232No Hit
GGAATACCGGGTGCTGTAGGCTT149450.19355175434040942No Hit
GGAATACCGGGTGCTGTAGGCTTT147240.19068959725046425No Hit
TAGCAGCACGTAAATATTGGC143960.18644168989525153No Hit
CACTAGATTGTGAGCTCCTGGA141900.183773796861185No Hit
TTCACAGTGGCTAAGTTCTGC138100.17885244077892634No Hit
TGAGGTAGTAGTTTGTGCTGTT137190.17767390550659598No Hit
TGTAAACATCCCCGACTGGA132360.17141860290730407No Hit
CCCATAAAGTAGAAAGCACTA127870.1656036321680037No Hit
TGGCTCAGTTCAGCAGGAAC126870.16430853846214616No Hit
AATGCACCCGGGCAAGGATTCT123740.16025489516281205No Hit
CTCGTACGACTCTTAGCGG119670.15498386377997186No Hit
ACCGGGTGCTGTAGGCTTA116660.15108563172534067No Hit
TGATGTAGTAGGTTGTATAGTT116020.15025677175359184No Hit
TATTGCACTTGTCCCGGCCTGTT115260.1492725005371401No Hit
TGTCAGTTTGTCAAATACCCCA109780.14217538702904078No Hit
AGCTCGGTCTGAGGCCCCTCAGT109280.14152784017611203No Hit
CCCCCCACTGCTAAATTTGACTGG108480.14049176521142598No Hit
TGAGGTAGTAAGTTGTATTGTT107780.1395851996173257No Hit
TCGTTTCCCGGCCAACGCACCA107130.1387433887085183No Hit
CACTAGATTGTGAGCTCCTGGAA106880.13841961528205393No Hit
TTCAAGTAATCCAGGATAGGC103450.13397744387096255No Hit
CCACCCTGAACGCGCCCGA99750.12918559715928965No Hit
TATTGCACTTGTCCCGGCCTGTAG97560.12634934194346165No Hit
AACCGGGCGGAAACACCA97180.12585720633523578No Hit
CAAAGAATTCTCCTTTTGGGCT96940.12554638384582997No Hit
ATCCCACCGCTGCCACCA96870.12545572728641993No Hit
TTCACAGTGGCTAAGTTCTG96160.1245362107552611No Hit
ACCCCCCACTGCTAAATTTGACTG95410.12356489047586794No Hit
TATTGCACTTGTCCCGGCCTGTAC94680.12261947207059193No Hit
TGTAAACATCCTACACTCTCAGCT90500.11720598038010742No Hit
TAGCTTATCAGACTGATGTTGACA90320.11697286351305305No Hit
ACCACCCTGAACGCGCCCGA82720.10713015134853575No Hit
ACTGGACTTGGAGTCAGAAGGCA82590.10696178916677426No Hit
TTATAAAGCAATGAGACTGATT81740.10586095951679536No Hit
TCGGACGACTCTTAGCGG81520.10557603890150669No Hit
TGATAACTGAATTCCATGGGTT80770.10460471862211354No Hit
CAAAGTGCTGTTCGTGCAGGTAG79340.10275273462273726No Hit
TTCAAGTAATCCAGGATAGGCTT78010.10103025999394673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)