FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11802745
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10589148.971760382860088No Hit
CCCCCCACTGCTAAATTTGACTG9378567.946083728827488No Hit
TTCAAGTAATCCAGGATAGGCT3952163.348509181550563No Hit
CCCCCACTGCTAAATTTGACTG3202262.7131485090968246No Hit
TAGCAGCACGTAAATATTGGCG3116862.640792459720175No Hit
AAGCTGCCAGTTGAAGAACTGT3079122.6088168472673092No Hit
AACATTCAACGCTGTCGGTGAGT2939662.4906578935662846No Hit
AACATTCAACGCTGTCGGTGAG1841841.5605183370478648No Hit
TATTGCACTTGTCCCGGCCTGTAA1527531.2942158794416045No Hit
TAGCTTATCAGACTGATGTTGAC1524751.2918604951644723No Hit
TATTGCACTTGTCCCGGCCTGTAT1459211.236331039940285No Hit
TAGCTTATCAGACTGATGTTGACT1339351.1347783926535733No Hit
TAGCTTATCAGACTGATGTTGA1280141.0846120965927841No Hit
TGAGGTAGTAGGTTGTATAGTT1201181.0177124050379804No Hit
TCGTACGACTCTTAGCGG1193951.0115867113963743No Hit
CATAAAGTAGAAAGCACTACT1163510.9857961008223087No Hit
TATTGCACTTGTCCCGGCCTGT1113020.943017916594826No Hit
TGAGGTAGTAGATTGTATAGTT950350.805194045961342No Hit
GCCTGTCTGAGCGTCGCT925530.7841650395734213No Hit
TTCAAGTAATTCAGGATAGGTT877410.7433948628052203No Hit
CTCCCACTGCTTCACTTGACTAG780890.6616172763200425No Hit
ACTGGACTTGGAGTCAGAAGGC710240.601758319780695No Hit
AGCAGCATTGTACAGGGCTATGA704490.5968865717254758No Hit
CCCATAAAGTAGAAAGCACTAC689410.5841098829128308No Hit
CAACGGAATCCCAAAAGCAGCTGT681140.5771030383186284No Hit
TATTGCACTTGTCCCGGCCTGTA663470.5621319447298065No Hit
TGTAAACATCCTTGACTGGAAGCT643030.5448139394691659No Hit
CATTGCACTTGTCTCGGTCTGA541950.45917284496106625No Hit
TTCTCACTACTGCACTTGACTAG532790.451411938493969No Hit
CGCCTGTCTGAGCGTCGCT525320.44508290232484055No Hit
ATCCCGGACGAGCCCCCA520100.44066020235123277No Hit
CCCATAAAGTAGAAAGCACT487860.4133445228207506No Hit
CCCCCACTGCTAAATTTGACTGGCTT453180.3839615275937928No Hit
TGTAAACATCCCCGACTGGAAGCT435720.3691683587165528No Hit
CTTCTCACTACTGCACTTGACTAG419130.35511230650158077No Hit
CAACGGAATCCCAAAAGCAGCT401880.3404970623359227No Hit
TTAATGCTAATCGTGATAGGGGTT398770.33786208208344753No Hit
CTACGCCTGTCTGAGCGTCGCT379900.3218742758570146No Hit
CAACACCAGTCGATGGGCTGT364630.3089366075434147No Hit
CACCCGGCTGTGTGCACATGTGC340530.2885176287380605No Hit
TGTAAACATCCTACACTCTCAGCT330190.27975695484397906No Hit
CCCCCCACTGCTAAATTTGACTGGCT313020.2652094915208284No Hit
TGAGGTAGTAGTTTGTACAGTT311630.26403179938226234No Hit
AGCTCGGTCTGAGGCCCCTCAGT301460.25541515977850915No Hit
ACCGGGTGCTGTAGGCTTA300650.2547288787481217No Hit
CCCCCCACTGCTAAATTTGACTGG280380.23755490777780933No Hit
ACCGGGTGCTGTAGGCTT279080.23645346908706408No Hit
TCCTGTCTGAGCGTCGCT262790.2226515950314948No Hit
CACTAGATTGTGAGCTCCTGGA257900.21850849103323No Hit
CTCCCACTGCTTCACTTGACTAGCCT254570.2156871134638595No Hit
TGAGGTAGTAGTTTGTGCTGTT252110.21360285255675693No Hit
TTCAAGTAATCCAGGATAGGCTT236250.20016530052966494No Hit
AACCGGGCGGAAACACCA234180.19841147122978595No Hit
AACATTCAACGCTGTCGGTGAGTT234150.19838605341384566No Hit
CACTAGATTGTGAGCTCCTGGAA231690.19630179250674312No Hit
AACGGAATCCCAAAAGCAGCTG231540.19617470342704174No Hit
CAAAGAATTCTCCTTTTGGGCT231080.1957849635826242No Hit
TACGCCTGTCTGAGCGTCGCT212630.18015300677935514No Hit
TGAGAACTGAATTCCATGGGTT205620.17421371045464423No Hit
TCCCACTGCTTCACTTGACTAG204520.1732817238701675No Hit
CAAAGAATTCTCCTTTTGGGCTT199060.16865568136903744No Hit
TGTCAGTTTGTCAAATACCCCAA194510.1648006459514291No Hit
TATTGCACTTGTCCCGGCCTGTT192680.16325015917907232No Hit
ACCGGGTGCTGTAGGCTTT190920.16175898064390953No Hit
TATTGCACTTGTCCCGGCCTGTAG187880.15918330862862834No Hit
ACTGGACTTGGAGTCAGAAGGCA183450.15542994447478106No Hit
TTCAAGTAATCCAGGATAGGC180420.15286274506481332No Hit
TCGTTTCCCGGCCAATGCACCA179360.15196464890159025No Hit
ACTGGACTTGGAGTCAGAAGG172710.14633036636816266No Hit
TAGCTTATCAGACTGATGTTGACA171400.145220455072104No Hit
TAGCAGCACGTAAATATTGGC169710.14378858477413517No Hit
ATCCCACCGCTGCCACCA168120.14244144052930058No Hit
TCGTTTCCCGGGCGGCGCACCA167580.14198391984237566No Hit
GGAATACCGGGTGCTGTAGGCTTT167240.1416958512617192No Hit
TTCACAGTGGCTAAGTTCCG166890.14139931007574935No Hit
ACCCCCCACTGCTAAATTTGACTG166740.14127222099604794No Hit
ACTGGACTTGGAGTCAGAAGGCT165890.14055204954440684No Hit
CGCGACCTCAGATCAGACGTGGCG159080.13478220532596444No Hit
TTCACAGTGGCTAAGTTCCGC156280.13240987583820543No Hit
CGCGACCTCAGATCAGACGTGG155990.13216417028411612No Hit
CCCATAAAGTAGAAAGCACTA150870.1278261963636425No Hit
TTCACAGTGGCTAAGTTCTGC149410.12658919598788249No Hit
ACCACAGGGTAGAACCACGGAC146760.12434395557982486No Hit
TGTAAACATCCCCGACTGGAAGC145950.12365767454943745No Hit
CCCCCCACTGCTAAATTTGACTGT144730.1226240167011996No Hit
ACGCCTGTCTGAGCGTCGCT144530.1224545645949311No Hit
TGTCAGTTTGTCAAATACCCCA144450.1223867837524237No Hit
AGCAGCATTGTACAGGGCTATGT142160.12044655713564939No Hit
TTATAAAGCAATGAGACTGATT140810.11930275541833701No Hit
ATACCGGGTGCTGTAGGCTTA139140.11788783033099502No Hit
TATTGCACTTGTCCCGGCCTGTTT138750.11755739872377147No Hit
TGAGGTAGTAGGTTGTATAGT135360.1146851855225204No Hit
TGAGGTAGTAGGTTGTGTGGTT134520.11397348667619271No Hit
CCCCCACTGCTAAATTTGACTGGCT134190.11369389070084969No Hit
TATTGCACTTGTCCCGGCCTGTAAT127520.10804266295679522No Hit
GGAATACCGGGTGCTGTAGGCTT127170.10774612177082533No Hit
TGAGAACTGAATTCCATAGGCTGT124030.1050857237024099No Hit
CGCGACCTCAGATCAGACGTGGC123660.10477223730581317No Hit
TGTAAACATCCTACACTCAGCT120590.1021711474745917No Hit
GACCCGCGGGCGCTCTCCAGTCCT120060.10172209939298019No Hit
CAAAGTGCTGTTCGTGCAGGTAG119100.1009087292828914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)