FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10066657
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8090858.037275929834502No Hit
CAACGGAATCCCAAAAGCAGCTG7073607.026761714440057No Hit
TTCAAGTAATCCAGGATAGGCT3521433.498112630637957No Hit
AAGCTGCCAGTTGAAGAACTGT3347703.325532994717114No Hit
TAGCAGCACGTAAATATTGGCG3132593.1118473590587223No Hit
AACATTCAACGCTGTCGGTGAGT3067763.0474466349653118No Hit
CCCCCACTGCTAAATTTGACTG2984762.964996224665249No Hit
TAGCTTATCAGACTGATGTTGAC2450522.434293728295302No Hit
TAGCTTATCAGACTGATGTTGACT2005761.992478734499447No Hit
AACATTCAACGCTGTCGGTGAG1808791.796812983694587No Hit
TAGCTTATCAGACTGATGTTGA1477071.4672894884567935No Hit
TGAGGTAGTAGGTTGTATAGTT1442721.4331669391338158No Hit
GCCTGTCTGAGCGTCGCT1276801.2683455888086779No Hit
TGTAAACATCCTTGACTGGAAGCT1254651.2463422564213722No Hit
TATTGCACTTGTCCCGGCCTGTAA1087721.0805175938745106No Hit
TGAGGTAGTAGATTGTATAGTT968060.9616499300611911No Hit
TATTGCACTTGTCCCGGCCTGTAT955090.9487658117287596No Hit
CATAAAGTAGAAAGCACTACT917820.9117425973687193No Hit
TATTGCACTTGTCCCGGCCTGT763920.7588616558605304No Hit
AGCAGCATTGTACAGGGCTATGA671390.6669443490525206No Hit
TTCAAGTAATTCAGGATAGGTT631590.6274078872459845No Hit
TGTAAACATCCCCGACTGGAAGCT630770.6265933169273573No Hit
ACTGGACTTGGAGTCAGAAGGC589600.5856959266616515No Hit
TATTGCACTTGTCCCGGCCTGTA581290.5774409518472716No Hit
CCCCCACTGCTAAATTTGACTGGCTT532110.528586600298391No Hit
CCCATAAAGTAGAAAGCACTAC504730.5013878986837438No Hit
CAACGGAATCCCAAAAGCAGCTGT452790.44979182264777673No Hit
TTCTCACTACTGCACTTGACTAG450600.44761632386997985No Hit
TGTCAGTTTGTCAAATACCCCAA444300.4413580397146739No Hit
CGCCTGTCTGAGCGTCGCT415560.41280834342523043No Hit
TGAGGTAGTAGTTTGTACAGTT411690.4089639688726853No Hit
TGTAAACATCCTACACTCTCAGCT368290.3658513446916886No Hit
CATTGCACTTGTCTCGGTCTGA346000.34370893932315366No Hit
TTAATGCTAATCGTGATAGGGGTT342870.3405996648142477No Hit
TAGCTTATCAGACTGATGTTGACA325670.323513555691825No Hit
CTCCCACTGCTTCACTTGACTAG314240.31215924015291274No Hit
CTTCTCACTACTGCACTTGACTAG307570.3055334059757872No Hit
CCCATAAAGTAGAAAGCACT307080.3050466505414856No Hit
TGAGGTAGTAGTTTGTGCTGTT289530.2876128589659904No Hit
AACATTCAACGCTGTCGGTGAGTT278650.2768049015676207No Hit
TGTCAGTTTGTCAAATACCCCA276110.2742817203367513No Hit
CCCCCCACTGCTAAATTTGACTGGCT273860.2720466188527135No Hit
CAAAGAATTCTCCTTTTGGGCT264730.2629770737197066No Hit
TCGTACGACTCTTAGCGG255040.25335123666178355No Hit
CTCCCACTGCTTCACTTGACTAGCCT254130.2524472622837949No Hit
CAACACCAGTCGATGGGCTGT249880.24822540392505674No Hit
CCCCCCACTGCTAAATTTGACTGG247860.24621877948160942No Hit
CTACGCCTGTCTGAGCGTCGCT230640.22911280279043975No Hit
TTCACAGTGGCTAAGTTCCG222760.22128498070412053No Hit
AACCGGGCGGAAACACCA203010.20166575656645497No Hit
GGAATACCGGGTGCTGTAGGCTTT197440.19613263867041464No Hit
CAACGGAATCCCAAAAGCAGCT197130.19582469135483604No Hit
TAGCAGCACGTAAATATTGGC192050.1907783288930973No Hit
TTCAAGTAATCCAGGATAGGCTT190160.18890084364650547No Hit
TACGCCTGTCTGAGCGTCGCT189470.18821541252473387No Hit
CGCGACCTCAGATCAGACGTGGCG183950.182731963550561No Hit
ACTGGACTTGGAGTCAGAAGGCA182670.18146043915075283No Hit
CAAAGAATTCTCCTTTTGGGCTT182170.18096374993207776No Hit
TTATAAAGCAATGAGACTGATT176570.1754008306829169No Hit
TGTAAACATCCCCGACTGGAAGC174720.17356308057381908No Hit
CGCGACCTCAGATCAGACGTGG174310.17315579541450554No Hit
AACGGAATCCCAAAAGCAGCTG169710.16858625460269483No Hit
TCCTGTCTGAGCGTCGCT167620.16651009366863298No Hit
ACCGGGTGCTGTAGGCTT166510.16540744360317433No Hit
TGTAAACATCCTACACTCAGCT161140.16007300139460398No Hit
ACTGGACTTGGAGTCAGAAGGCT150160.14916570615249927No Hit
CACCCGGCTGTGTGCACATGTGC149240.14825179799013716No Hit
TATTGCACTTGTCCCGGCCTGTT148770.14778491012458259No Hit
ACTGGACTTGGAGTCAGAAGG147790.1468113992559794No Hit
TTCAAGTAATCCAGGATAGGC147080.1461061005654608No Hit
GGAATACCGGGTGCTGTAGGCTT145810.14484450995002612No Hit
ACCCCCCACTGCTAAATTTGACTG144970.14401007206265198No Hit
GGGCTACGCCTGTCTGAGCGTCGCT144170.14321536931277187No Hit
TAGCAGCACGTAAATATTGGCGT134340.13345045927361981No Hit
TATTGCACTTGTCCCGGCCTGTAG133870.13298357140806527No Hit
TTCACAGTGGCTAAGTTCCGC132720.1318411862051126No Hit
TTCACAGTGGCTAAGTTCTGC132020.13114582129896746No Hit
TGAGGTAGTAGGTTGTGTGGTT131480.1306093969427984No Hit
CCCCCACTGCTAAATTTGACTGGCT127790.1269438305089763No Hit
CACTAGATTGTGAGCTCCTGGA126940.12609945883722867No Hit
AGCTCGGTCTGAGGCCCCTCAGT126680.12584118044351766No Hit
CACTAGATTGTGAGCTCCTGGAA125800.12496700741864951No Hit
AGCAGCATTGTACAGGGCTATGT124870.12404316547191387No Hit
TGAGGTAGTAAGTTGTATTGTT123290.12247362754090063No Hit
TGAGGTAGTAGGTTGTATAGT121440.12063587743180283No Hit
TTCACAGTGGCTAAGTTCTG120010.11921534626639212No Hit
TCCCACTGCTTCACTTGACTAG112560.11181467690813346No Hit
AGCAGCATTGTACAGGGCTATG110120.10939083352099908No Hit
GCGTTCGCGCTTTCCCCTG108300.10758288476502179No Hit
ACCGGGTGCTGTAGGCTTA107160.10645043334644262No Hit
ATCACATTGCCAGGGATTTCC106520.10581467114653852No Hit
ATGCACCTGGGCAAGGATTCTGA105990.10528818057474294No Hit
ACCACAGGGTAGAACCACGGAC105790.10508950488727291No Hit
CCCATAAAGTAGAAAGCACTA104540.10384778184058521No Hit
TCGTTTCCCGGCCAATGCACCA104070.10338089397503064No Hit
GACCCGCGGGCGCTCTCCAGTCCT103890.10320208585630761No Hit
TGTCAGTTTGTCAAATACCCC103700.10301334395321107No Hit
CAAAGTGCTGTTCGTGCAGGTAG102910.10222857498770445No Hit
ACGCCTGTCTGAGCGTCGCT101050.10038089109423316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)