FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11346038
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6326795.576210832362804No Hit
CCCCCCACTGCTAAATTTGACTG5295294.667082905944789No Hit
AAGCTGCCAGTTGAAGAACTGT4611644.064537770805985No Hit
TAGCAGCACGTAAATATTGGCG4541764.002947989421505No Hit
TTCAAGTAATCCAGGATAGGCT4374803.8557953005269328No Hit
TAGCTTATCAGACTGATGTTGAC2669942.3531914841110178No Hit
AACATTCAACGCTGTCGGTGAGT2662682.3467927747113135No Hit
TAGCTTATCAGACTGATGTTGA2552082.249313813332901No Hit
CATAAAGTAGAAAGCACTACT2132801.8797751250260224No Hit
CCCCCACTGCTAAATTTGACTG2045481.802814339243355No Hit
TAGCTTATCAGACTGATGTTGACT1718251.5144052928431933No Hit
AACATTCAACGCTGTCGGTGAG1414441.2466378131291291No Hit
TGAGGTAGTAGGTTGTATAGTT1334921.1765516738089543No Hit
TGTAAACATCCTTGACTGGAAGCT1261211.1115862647384047No Hit
TTCAAGTAATTCAGGATAGGTT1257931.1086953877644337No Hit
TGAGGTAGTAGATTGTATAGTT1100320.9697834609755405No Hit
TATTGCACTTGTCCCGGCCTGT963130.8488690060794789No Hit
TATTGCACTTGTCCCGGCCTGTAA943690.8317352718191143No Hit
TATTGCACTTGTCCCGGCCTGTAT872780.7692376845556131No Hit
TTCTCACTACTGCACTTGACTAG850240.7493717190088734No Hit
CGCCTGTCTGAGCGTCGCT729400.6428675807361125No Hit
AGCAGCATTGTACAGGGCTATGA648400.5714770213179262No Hit
TGTCAGTTTGTCAAATACCCCAA550700.48536766755055816No Hit
TATTGCACTTGTCCCGGCCTGTA541000.4768184277189976No Hit
CCCATAAAGTAGAAAGCACTAC532650.4694590305444068No Hit
CTTCTCACTACTGCACTTGACTAG510570.4499984928659678No Hit
CTCCCACTGCTTCACTTGACTAG497140.4381617618414463No Hit
CATTGCACTTGTCTCGGTCTGA491060.43280306306042693No Hit
TGTAAACATCCCCGACTGGAAGCT480770.42373381791952397No Hit
TGAGGTAGTAGTTTGTACAGTT437870.3859232623758179No Hit
CTACGCCTGTCTGAGCGTCGCT436180.38443375564227794No Hit
TGTAAACATCCTACACTCTCAGCT433500.38207169762696014No Hit
TTCACAGTGGCTAAGTTCCG428100.37731232699908107No Hit
TGTCAGTTTGTCAAATACCCCA401580.3539385290266082No Hit
CAACGGAATCCCAAAAGCAGCTGT400510.3529954685503433No Hit
TGATGTAGTAGGTTGTATAGTT400200.3527222454217058No Hit
ACTGGACTTGGAGTCAGAAGGC355790.31358082883205574No Hit
TCGTACGACTCTTAGCGG349430.3079753478703315No Hit
TGATGTAGTAGATTGTATAGTT336060.2961914987416753No Hit
CCCCCACTGCTAAATTTGACTGGCTT322470.2842137493281796No Hit
TGAGGTAGTAGTTTGTGCTGTT318380.2806089667600267No Hit
TTATAAAGCAATGAGACTGATT314820.2774713076053509No Hit
TGTAAACATCCTACACTCAGCT309120.27244752749814516No Hit
GCCTGTCTGAGCGTCGCT306850.27044682910457374No Hit
CCCATAAAGTAGAAAGCACT294670.25971180424391316No Hit
TAGCTTATCAGACTGATGTTGACA284020.25032526772781827No Hit
CAACACCAGTCGATGGGCTGT257000.22651078728980106No Hit
TACGCCTGTCTGAGCGTCGCT255210.2249331440631523No Hit
CAAAGAATTCTCCTTTTGGGCT252410.22246532225610385No Hit
TAGCAGCACGTAAATATTGGCGT248190.21874596224690945No Hit
TTCACAGTGGCTAAGTTCCGC245800.21663950006160743No Hit
CTCCCACTGCTTCACTTGACTAGCCT245680.21653373626987676No Hit
AACATTCAACGCTGTCGGTGAGTT245560.21642797247814613No Hit
ACGCCTGTCTGAGCGTCGCT244550.21553779389774652No Hit
TAGCAGCACGTAAATATTGGC233960.20620413927751696No Hit
TTCAAGTAATCCAGGATAGGCTT221640.19534572332650393No Hit
TTCAAGTAATCCAGGATAGGC214790.1893083735485462No Hit
AACCGGGCGGAAACACCA212790.18754564368636875No Hit
CCCCCCACTGCTAAATTTGACTGG211460.18637342832802076No Hit
CAAAGAATTCTCCTTTTGGGCTT204720.1804330286924828No Hit
TTCACAGTGGCTAAGTTCTGC202110.17813266622234122No Hit
TAGCAGCACATAATGGTTTGT201720.17778893389921663No Hit
CCCCCCACTGCTAAATTTGACTGGCT201020.17717197844745453No Hit
GGGCTACGCCTGTCTGAGCGTCGCT199730.17603501768635008No Hit
TCCCACTGCTTCACTTGACTAG197280.17387567360518272No Hit
TGTAAACATCCCCGACTGGAAGC186840.16467422372461646No Hit
TGTCAGTTTGTCAAATACCCC184620.16271759357759952No Hit
AGAATTACTCTGAGACCT176290.15537582370163047No Hit
TGTCAGTTTGTCAAATACC172810.15230867374144172No Hit
CAACGGAATCCCAAAAGCAGCT166030.1463330195086602No Hit
GCGTTCGCGCTTTCCCCTG161850.14264891409670935No Hit
TTCACAGTGGCTAAGTTCTG161610.14243738651324805No Hit
TATTGCACTTGTCCCGGCCTGTT160610.14155602158215935No Hit
CAAAGTGCTGTTCGTGCAGGT152720.13460205227586933No Hit
ACCGGGTGCTGTAGGCTT145220.12799181529270395No Hit
CACCCGGCTGTGTGCACATGTGC145030.12782435595579708No Hit
TAGCTTATCAGACTGATGTTG142090.12523314305839622No Hit
TGAGGTAGTAGGTTGTATAGT137270.12098496409054861No Hit
AGCAGCATTGTACAGGGCTATG136670.12045614513189538No Hit
TAGTGGGTTATCAGAACT135610.11952189830494134No Hit
TGTCAGTTTGTCAAATACCC131450.11585542019161227No Hit
ATCCCACCGCTGCCACCA131370.11578491099712515No Hit
AGCAGCATTGTACAGGGCTATGT128910.11361675326664691No Hit
TGATGTAGTAGTTTGTACAGTT128760.1134845485269836No Hit
ACCCCCCACTGCTAAATTTGACTG125820.11089333562958276No Hit
CATAAAGTAGAAAGCACTAC125810.11088452198027188No Hit
TGTAAACATCCTTGACTGGAAGC122220.10772042187766337No Hit
ATCACATTGCCAGGGATTTCC121490.1070770254779686No Hit
TAGCAGCACATCATGGTTTACA121050.10668922490828957No Hit
CCCATAAAGTAGAAAGCACTA120540.10623972879343434No Hit
TGAGGTAGTAGATTGTATAGT120290.10601938756066213No Hit
TGTAGTGTTTCCTACTTTATGGA118570.10450343987918953No Hit
AACGGAATCCCAAAAGCAGCTG118340.10430072594503914No Hit
ACTTGACTTGGAGTCAGAAGGC118150.10413326660813227No Hit
ACTGGACTTGGAGTCAGAAGG116700.10285528745805363No Hit
CATAAAGTAGAAAGCACTACTA116390.10258206432941615No Hit
AGCTCGGTCTGAGGCCCCTCAGT115390.10170069939832742No Hit
TGAGGTAGTAAGTTGTATTGTT115320.1016390038531512No Hit
CAAAGTGCTGTTCGTGCAGGTAG114800.10118069408898507No Hit
ATCCCGGACGAGCCCCCA114350.10078407986999516No Hit
ATCACATTGCCAGGGATTTCCA114280.10072238432481893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)