FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10184978
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8308258.157356844560686No Hit
CCCCCCACTGCTAAATTTGACTG7964047.819398333506464No Hit
AAGCTGCCAGTTGAAGAACTGT4528604.446352265071166No Hit
TTCAAGTAATCCAGGATAGGCT3740203.6722710643066683No Hit
TAGCAGCACGTAAATATTGGCG3587493.5223345597801No Hit
AACATTCAACGCTGTCGGTGAGT3570783.5059280442235616No Hit
TAGCTTATCAGACTGATGTTGAC2738632.6888914242131894No Hit
TAGCTTATCAGACTGATGTTGACT2264422.2232939531140863No Hit
CCCCCACTGCTAAATTTGACTG2118762.0802794075745674No Hit
AACATTCAACGCTGTCGGTGAG1997791.961506446062034No Hit
TAGCTTATCAGACTGATGTTGA1751061.71925751827839No Hit
TATTGCACTTGTCCCGGCCTGTAA1297791.274219738128055No Hit
TGTAAACATCCTTGACTGGAAGCT1284311.2609845598095548No Hit
TATTGCACTTGTCCCGGCCTGTAT1179531.1581075580133802No Hit
TGAGGTAGTAGGTTGTATAGTT1138491.117812920165365No Hit
AGCAGCATTGTACAGGGCTATGA912140.8955738539641421No Hit
TATTGCACTTGTCCCGGCCTGT799670.7851465167622356No Hit
CATAAAGTAGAAAGCACTACT794600.780168597320485No Hit
TGAGGTAGTAGATTGTATAGTT784260.770016390806146No Hit
TTCAAGTAATTCAGGATAGGTT752240.7385779331089375No Hit
TGTAAACATCCCCGACTGGAAGCT604370.5933935252486554No Hit
TATTGCACTTGTCCCGGCCTGTA579300.5687788427230771No Hit
CAACGGAATCCCAAAAGCAGCTGT527040.5174679807850346No Hit
CCCATAAAGTAGAAAGCACTAC510090.5008258240714903No Hit
TTCTCACTACTGCACTTGACTAG474900.4662749394254951No Hit
TGTCAGTTTGTCAAATACCCCAA411270.4038005776742964No Hit
TGTAAACATCCTACACTCTCAGCT395610.3884249921796591No Hit
ACTGGACTTGGAGTCAGAAGGC383790.3768196651971168No Hit
TAGCTTATCAGACTGATGTTGACA376900.37005480031473803No Hit
CGCCTGTCTGAGCGTCGCT353140.3467263257711504No Hit
TGATGTAGTAGGTTGTATAGTT351170.3447921046073934No Hit
CTTCTCACTACTGCACTTGACTAG349630.3432800738499386No Hit
AACATTCAACGCTGTCGGTGAGTT335640.32954415807280096No Hit
TGAGGTAGTAGTTTGTACAGTT306740.3011690354166695No Hit
CATTGCACTTGTCTCGGTCTGA279310.27423721484719943No Hit
TAGCAGCACGTAAATATTGGC272800.26784544846341346No Hit
TGTCAGTTTGTCAAATACCCCA257260.2525876835472791No Hit
CAAAGAATTCTCCTTTTGGGCT252910.24831668757654657No Hit
TGATGTAGTAGATTGTATAGTT241750.23735937377577054No Hit
CCCATAAAGTAGAAAGCACT233270.2290333862282275No Hit
CAACACCAGTCGATGGGCTGT223730.21966665023724158No Hit
CTCCCACTGCTTCACTTGACTAG223020.21896954514776568No Hit
TAGCAGCACGTAAATATTGGCGT220180.2161811247898621No Hit
CTACGCCTGTCTGAGCGTCGCT213900.2100151811815401No Hit
TTCACAGTGGCTAAGTTCCG206810.2030539486683231No Hit
TTCAAGTAATCCAGGATAGGCTT205960.2022193862372604No Hit
ACCCCCCACTGCTAAATTTGACTG205930.20218993109263464No Hit
TTCACAGTGGCTAAGTTCCGC201890.19822330494970142No Hit
TGTAAACATCCCCGACTGGAAGC198370.1947672346469477No Hit
CACCCGGCTGTGTGCACATGTGC196190.19262682747081045No Hit
CAACGGAATCCCAAAAGCAGCT194050.19052569382084084No Hit
CCCCCCACTGCTAAATTTGACTGG193890.1903685997161702No Hit
TGTAAACATCCTACACTCAGCT189830.18638233681015315No Hit
CAAAGAATTCTCCTTTTGGGCTT184100.18075640418663644No Hit
TACGCCTGTCTGAGCGTCGCT175610.17242059825755146No Hit
TGAGGTAGTAGTTTGTGCTGTT175440.17225368577133893No Hit
AGCAGCATTGTACAGGGCTATGT171830.168709250034708No Hit
CCCCCACTGCTAAATTTGACTGGCTT168490.1654299105997087No Hit
TATTGCACTTGTCCCGGCCTGTT160620.15770284432622242No Hit
TCGTTTCCCGGCCAATGCACCA158010.15514024674378285No Hit
TTATAAAGCAATGAGACTGATT156640.15379512847254062No Hit
ATCACATTGCCAGGGATTTCC155860.15302929471227136No Hit
TTCAAGTAATCCAGGATAGGC153360.15057469932679285No Hit
ATCACATTGCCAGGGATTTCCA149600.1468829878670332No Hit
AACGGAATCCCAAAAGCAGCTG140940.13838026945173568No Hit
TAGCAGCACATCATGGTTTACA140420.13786971361155617No Hit
GACCCGCGGGCGCTCTCCAGTCCT138800.1362791358017661No Hit
AGCTCGGTCTGAGGCCCCTCAGT136620.13413872862562884No Hit
CAAAGTGCTGTTCGTGCAGGTAG135190.13273470006513513No Hit
TTCACAGTGGCTAAGTTCTGC133870.13143867370160248No Hit
TATTGCACTTGTCCCGGCCTGTAG133480.13105575682146786No Hit
CCCCCCACTGCTAAATTTGACTGGCT131800.1294062687224263No Hit
ACTTGACTTGGAGTCAGAAGGC131250.12886625773762103No Hit
CCCCCCACTGCTAAATTTGACTGTA126350.12405525078208318No Hit
CGCGACCTCAGATCAGACGTGGCG123500.12125701204263771No Hit
ACGCCTGTCTGAGCGTCGCT122080.11986280186368592No Hit
ACTGGACTTGGAGTCAGAAGGCA122070.11985298348214399No Hit
AACCGGGCGGAAACACCA120750.11855695711861135No Hit
ACCACAGGGTAGAACCACGGAC120410.11822313214618627No Hit
CACTAGATTGTGAGCTCCTGGA118260.11611218011467477No Hit
AACATTCAACGCTGTCGGTGAGA117440.11530707282823782No Hit
ATGCACCTGGGCAAGGATTCTGA116390.11427614276633687No Hit
TGAGGTAGTAGGTTGTGTGGTT115540.11344158033527417No Hit
AGCAGCATTGTACAGGGCTATG112920.11086916437129271No Hit
TATTGCACTTGTCCCGGCCTGTAAT112410.11036842691265508No Hit
TCGTTTCCCGGGCGGCGCACCA111340.1093178600876703No Hit
GCCTGTCTGAGCGTCGCT110740.10872875719515546No Hit
CCAAGTCCTTCTGATCGAGGCCC109100.10711854262228156No Hit
TCGTACGACTCTTAGCGG106880.10493886191997666No Hit
CACTAGATTGTGAGCTCCTGGAA106330.10439885093517139No Hit
CAAAGTGCTGTTCGTGCAGGT105500.10358392526719253No Hit
CTCCCACTGCTTCACTTGACTAGCCT102430.10056968213382493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)