FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698194
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6162336.354100567590214No Hit
CCCCCCACTGCTAAATTTGACTG4832334.982711214067279No Hit
AAGCTGCCAGTTGAAGAACTGT4079764.20672137513438No Hit
TTCAAGTAATCCAGGATAGGCT3449053.556383796818253No Hit
AACATTCAACGCTGTCGGTGAGT2970023.0624464719926205No Hit
TAGCAGCACGTAAATATTGGCG2683102.766597574764951No Hit
TAGCTTATCAGACTGATGTTGAC2158902.2260845679102728No Hit
TAGCTTATCAGACTGATGTTGA1949302.0099618547535756No Hit
TAGCTTATCAGACTGATGTTGACT1902121.9613136218970255No Hit
CCCCCACTGCTAAATTTGACTG1882731.9413202086904016No Hit
TGAGGTAGTAGGTTGTATAGTT1862671.920635945207943No Hit
AACATTCAACGCTGTCGGTGAG1836201.8933422037133922No Hit
CATAAAGTAGAAAGCACTACT1610311.6604225487755764No Hit
TGAGGTAGTAGATTGTATAGTT1327371.3686775084103286No Hit
CGCCTGTCTGAGCGTCGCT1154911.190850585170806No Hit
GCCTGTCTGAGCGTCGCT1082501.116187199389907No Hit
TGTAAACATCCTTGACTGGAAGCT910130.9384530769337054No Hit
TATTGCACTTGTCCCGGCCTGT866200.8931559834748615No Hit
TATTGCACTTGTCCCGGCCTGTAA846280.8726160767664578No Hit
TATTGCACTTGTCCCGGCCTGTAT773940.7980248693725862No Hit
TTCAAGTAATTCAGGATAGGTT748250.7715354013334854No Hit
AGCAGCATTGTACAGGGCTATGA585590.6038134522778158No Hit
TGAGGTAGTAGTTTGTACAGTT540800.557629595778348No Hit
CCCATAAAGTAGAAAGCACTAC514900.5309235925781646No Hit
TCGTACGACTCTTAGCGG507650.5234479739217426No Hit
CAACACCAGTCGATGGGCTGT492680.5080121102959995No Hit
TATTGCACTTGTCCCGGCCTGTA476490.49131828049634807No Hit
TTAATGCTAATCGTGATAGGGGTT459060.4733458621264949No Hit
CCCATAAAGTAGAAAGCACT456050.47024219148431146No Hit
TGTAAACATCCCCGACTGGAAGCT420710.4338024172335591No Hit
ACTGGACTTGGAGTCAGAAGGC416580.4295438923989353No Hit
CAACGGAATCCCAAAAGCAGCTGT384640.39660992551809127No Hit
CATTGCACTTGTCTCGGTCTGA377890.38964986676900876No Hit
AACCGGGCGGAAACACCA374480.3861337482009537No Hit
TGTCAGTTTGTCAAATACCCCAA360180.37138873485104545No Hit
TACGCCTGTCTGAGCGTCGCT327860.3380629424406235No Hit
TCCTGTCTGAGCGTCGCT318670.3285869513437244No Hit
TTCACAGTGGCTAAGTTCCG296120.30533519952271526No Hit
TGAGGTAGTAGTTTGTGCTGTT287040.29597263160543086No Hit
ATCCCGGACGAGCCCCCA281870.290641742163541No Hit
TGTAAACATCCTACACTCTCAGCT269810.27820643719851346No Hit
CTACGCCTGTCTGAGCGTCGCT261160.269287250801541No Hit
TTATAAAGCAATGAGACTGATT252510.26036806440456856No Hit
ACGCCTGTCTGAGCGTCGCT246180.2538410759776511No Hit
AACATTCAACGCTGTCGGTGAGTT245720.25336676086289883No Hit
TTCTCACTACTGCACTTGACTAG238300.2457158518379814No Hit
TAGCTTATCAGACTGATGTTGACA233420.24068398714234837No Hit
GGAATACCGGGTGCTGTAGGCTTT226100.23313619009889883No Hit
TTCACAGTGGCTAAGTTCCGC217030.2237839333797612No Hit
TGTCAGTTTGTCAAATACCCCA210680.2172363225565502No Hit
TGAGAACTGAATTCCATGGGTT198550.2047288392044952No Hit
TGAGGTAGTAGGTTGTATAGT191000.19694388460366952No Hit
ACCGGGTGCTGTAGGCTT187370.1932009196763851No Hit
CAACGGAATCCCAAAAGCAGCT183850.1895713779287154No Hit
GGAATACCGGGTGCTGTAGGCTT181030.1866636200513209No Hit
TAGCAGCACGTAAATATTGGC178490.1840445757220365No Hit
TTCAAGTAATCCAGGATAGGCTT178140.18368368378689887No Hit
AACGGAATCCCAAAAGCAGCTG176830.1823329168296695No Hit
GCGTTCGCGCTTTCCCCTG164740.16986667827020163No Hit
TTCAAGTAATCCAGGATAGGC162160.16720638914833008No Hit
CAAAGAATTCTCCTTTTGGGCT157900.162813818737798No Hit
TGAGGTAGTAGGTTGTGTGGTT156770.16164865334721085No Hit
TTCACAGTGGCTAAGTTCTG155270.1601019736251925No Hit
TGTAAACATCCTACACTCAGCT152700.1574519957014677No Hit
ATCCCACCGCTGCCACCA148300.15291506851688058No Hit
TGAGGTAGTAAGTTGTATTGTT144850.14935770515623836No Hit
TATTGCACTTGTCCCGGCCTGTT143840.1483162741434127No Hit
CACCCGGCTGTGTGCACATGTGC141040.14542913866231177No Hit
CTTCTCACTACTGCACTTGACTAG140760.14514042511420167No Hit
ACCACAGGGTAGAACCACGGAC138360.14266573755897233No Hit
TAGCAGCACGTAAATATTGGCGT131270.13535509807289894No Hit
AGCAGCATTGTACAGGGCTATGT130740.13480860457111912No Hit
ACCGGGTGCTGTAGGCTTT127890.13186991309928425No Hit
TTCACAGTGGCTAAGTTCTGC126530.13046759015132095No Hit
TGTAAACATCCCCGACTGGAAGC125930.12984891826251363No Hit
TGAGGTAGTAGATTGTATAGT125240.12913744559038517No Hit
ACTGGACTTGGAGTCAGAAGGCA123590.127436097896165No Hit
CGCGACCTCAGATCAGACGTGGCG122080.12587910697599985No Hit
ACTGGACTTGGAGTCAGAAGG120460.12420869287622005No Hit
AGAGGTAGTAGGTTGCATAGTT120120.12385811213922922No Hit
CAAAGAATTCTCCTTTTGGGCTT117790.12145560297102738No Hit
TTAGGTAGTAGGTTGTATAGTT117230.1208781758748072No Hit
CCCATAAAGTAGAAAGCACTA116480.12010483601379804No Hit
TGAGAACTGAATTCCATAGGCTGT116190.11980581126754114No Hit
CAAAGTGCTGTTCGTGCAGGT115150.11873344666027509No Hit
CTCCCACTGCTTCACTTGACTAG114350.11790855080853198No Hit
AGCAGCATTGTACAGGGCTATG113620.11715583334381638No Hit
ACTGGACTTGGAGTCAGAAGGCT112970.1164856054642751No Hit
CGCGACCTCAGATCAGACGTGG112790.11630000389763291No Hit
CACTAGATTGTGAGCTCCTGGA112150.11564008721623842No Hit
ACCGGGTGCTGTAGGCTTA111570.11504203772372465No Hit
TCGTTTCCCGGCCAATGCACCA110590.11403154030533932No Hit
TAGCTTATCAGACTGATGTTG102820.10601973934528429No Hit
CAAAGTGCTGTTCGTGCAGGTAG99500.10259642156055034No Hit
CATAAAGTAGAAAGCACTAC98190.10124565460332098No Hit
TCGACCGGACCTCGACCGGCT97370.1004001363552843No Hit
AACATTCAACGCTGTCGGTGA96990.10000831082570631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)