FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11649811
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG131005111.245255395130444No Hit
CAACGGAATCCCAAAAGCAGCTG124831910.715358386500863No Hit
TTCAAGTAATCCAGGATAGGCT4746784.074555372615058No Hit
CCCCCACTGCTAAATTTGACTG4249973.6481021022572815No Hit
AACATTCAACGCTGTCGGTGAGT2823372.4235328796321247No Hit
AAGCTGCCAGTTGAAGAACTGT2513692.157708824632434No Hit
TAGCAGCACGTAAATATTGGCG2334092.003543233448165No Hit
AACATTCAACGCTGTCGGTGAG1810871.554420067415686No Hit
TAGCTTATCAGACTGATGTTGAC1231811.0573647933000803No Hit
CATAAAGTAGAAAGCACTACT1229391.0552875063810048No Hit
TAGCTTATCAGACTGATGTTGA1160640.9962736734527281No Hit
TAGCTTATCAGACTGATGTTGACT964790.8281593581217754No Hit
GCCTGTCTGAGCGTCGCT936630.8039872921543534No Hit
CCCATAAAGTAGAAAGCACTAC871640.7482009793978631No Hit
CAACGGAATCCCAAAAGCAGCTGT834540.7163549691922041No Hit
TCGTACGACTCTTAGCGG808120.6936764896872576No Hit
AGCAGCATTGTACAGGGCTATGA754730.6478474200139384No Hit
TGAGGTAGTAGGTTGTATAGTT720530.6184907205790721No Hit
TTCAAGTAATTCAGGATAGGTT719670.6177525111780783No Hit
TATTGCACTTGTCCCGGCCTGTAA691470.5935461098896797No Hit
CCCATAAAGTAGAAAGCACT680910.5844815851518964No Hit
ACTGGACTTGGAGTCAGAAGGC649250.5573051785990348No Hit
TATTGCACTTGTCCCGGCCTGTAT608650.5224548278079362No Hit
TGAGGTAGTAGATTGTATAGTT588010.5047378021840869No Hit
CAACGGAATCCCAAAAGCAGCT522970.44890857027637615No Hit
TATTGCACTTGTCCCGGCCTGT508070.4361186632126478No Hit
CATTGCACTTGTCTCGGTCTGA496260.42598115969435035No Hit
CAACACCAGTCGATGGGCTGT492790.42300257059964325No Hit
CGCCTGTCTGAGCGTCGCT484040.4154917191360443No Hit
TTAATGCTAATCGTGATAGGGGTT451320.3874054265773067No Hit
ATCCCGGACGAGCCCCCA449980.38625519332459557No Hit
AACCGGGCGGAAACACCA446160.3829761701713444No Hit
TGAGAACTGAATTCCATGGGTT433180.37183435851448576No Hit
CACCCGTAGAACCGACCTTGCG402360.34537899370212954No Hit
AGCTCGGTCTGAGGCCCCTCAGT401560.34469228728260054No Hit
TGTAAACATCCTACACTCTCAGCT401330.3444948591869859No Hit
CACTAGATTGTGAGCTCCTGGA357640.30699210485045636No Hit
CACCCGGCTGTGTGCACATGTGC356380.30591054223969816No Hit
TGAGAACTGAATTCCATAGGCTGT356250.30579895244652466No Hit
CACTAGATTGTGAGCTCCTGGAA335670.28813342980414014No Hit
TGTAAACATCCTTGACTGGAAGCT330070.2833264848674369No Hit
CAAAGAATTCTCCTTTTGGGCT324580.2786139620634189No Hit
CTCCCACTGCTTCACTTGACTAG324380.27844228545853666No Hit
TTCTCACTACTGCACTTGACTAG319630.274364966092583No Hit
TATTGCACTTGTCCCGGCCTGTA298260.2560213208609135No Hit
TCGTTTCCCGGGCGGCGCACCA283070.24298248272010595No Hit
AACGGAATCCCAAAAGCAGCTG276690.23750599902436187No Hit
GACCCGCGGGCGCTCTCCAGTCCT276510.2373514900799678No Hit
TTCAAGTAATCCAGGATAGGCTT265400.22781485467875828No Hit
TTCACAGTGGCTAAGTTCCG265000.22747150146899378No Hit
CTACGCCTGTCTGAGCGTCGCT246490.21158283168714068No Hit
CTTCTCACTACTGCACTTGACTAG243910.20936820348415952No Hit
TCGTTTCCCGGCCAATGCACCA237370.20375437850450964No Hit
TGTAAACATCCCCGACTGGAAGCT237260.2036599563718244No Hit
ACCGGGTGCTGTAGGCTTA235390.2020547801161753No Hit
CCCCCCACTGCTAAATTTGACTGT230720.1980461313921745No Hit
AACATTCAACGCTGTCGGTGAGTT227960.19567699424479934No Hit
TACGCCTGTCTGAGCGTCGCT216310.18567683201040774No Hit
CCCCCACTGCTAAATTTGACTGGCTT215460.18494720643965812No Hit
CCCATAAAGTAGAAAGCACTA213090.18291283867180333No Hit
CCCCCCACTGCTAAATTTGACTGG208990.179393468271717No Hit
CAAAGAATTCTCCTTTTGGGCTT206770.17748785795752395No Hit
TTCAAGTAATCCAGGATAGGC204840.17583117872041015No Hit
TCCTGTCTGAGCGTCGCT202610.17391698457597293No Hit
CCCCCCACTGCTAAATTTGACTGTA198000.16995983883343688No Hit
ACTGGACTTGGAGTCAGAAGGCT184510.15838025183412846No Hit
ACCGGGTGCTGTAGGCTT175840.15093807101248252No Hit
ACCCCCCACTGCTAAATTTGACTG175820.1509209033519943No Hit
TTCACAGTGGCTAAGTTCCGC174810.1500539364973389No Hit
AGCAGCATTGTACAGGGCTATGT172890.1484058410904692No Hit
ACCACAGGGTAGAACCACGGAC162260.1392812295409771No Hit
CCCCCCACTGCTAAATTTGACTGA156390.1342425211876828No Hit
CCCCCCACTGCTAAATTTGACTGGCT149360.12820808852607135No Hit
TGTAAACATCCTACACTCAGCT147290.12643123566554No Hit
TAGCAGCACGTAAATATTGGC146810.12601921181382256No Hit
TGAGGTAGTAGTTTGTACAGTT146380.1256501071133257No Hit
ATCACATTGCCAGGGATTTCC145550.12493764920306431No Hit
CGCGACCTCAGATCAGACGTGGCG144740.12424235895329118No Hit
ACTGGACTTGGAGTCAGAAGG143710.12335822443814753No Hit
ACTGGACTTGGAGTCAGAAGGCA143310.12301487122838302No Hit
TCCCTGAGACCCTTTAACCTGTGA142750.12253417673471269No Hit
CGCGACCTCAGATCAGACGT142200.12206206607128647No Hit
ACCGGGTGCTGTAGGCTTT137810.11829376459412087No Hit
CAACGGAATCCCAAAAGCAGCTGA136950.11755555519312717No Hit
CGCGACCTCAGATCAGACGTGGC128970.11070565865832502No Hit
AGCTACATCTGGCTACTGGGTCTC128240.11007903905050477No Hit
TGTCAGTTTGTCAAATACCCCAA127090.10909189857243176No Hit
ACGCCTGTCTGAGCGTCGCT123690.10617339628943337No Hit
TGTCAGTTTGTCAAATACCCCA118730.10191581648835334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)