FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8618360
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG86988110.093347226154396No Hit
CAACGGAATCCCAAAAGCAGCTG4755505.517871149499441No Hit
AAGCTGCCAGTTGAAGAACTGT3303303.8328637931114504No Hit
TTCAAGTAATCCAGGATAGGCT3078693.572245763695181No Hit
TAGCAGCACGTAAATATTGGCG2712573.1474317619593517No Hit
TAGCTTATCAGACTGATGTTGAC2594833.010816443035566No Hit
CCCCCACTGCTAAATTTGACTG2485442.8838897423639764No Hit
AACATTCAACGCTGTCGGTGAGT2339392.7144259464677734No Hit
TAGCTTATCAGACTGATGTTGACT2137892.480622763495607No Hit
AACATTCAACGCTGTCGGTGAG1637391.8998858251453872No Hit
TAGCTTATCAGACTGATGTTGA1400381.6248799075462153No Hit
TGTAAACATCCTTGACTGGAAGCT1272391.476371374600272No Hit
TTAATGCTAATCGTGATAGGGGTT1077411.250133436059761No Hit
TATTGCACTTGTCCCGGCCTGTAA1074201.2464088295220899No Hit
TGAGGTAGTAGGTTGTATAGTT1047501.215428457386324No Hit
TATTGCACTTGTCCCGGCCTGTAT921341.0690432982609221No Hit
TGAGGTAGTAGATTGTATAGTT824110.9562260105170821No Hit
CATAAAGTAGAAAGCACTACT664210.7706918717714275No Hit
AGCAGCATTGTACAGGGCTATGA637930.7401988313321792No Hit
TATTGCACTTGTCCCGGCCTGTA580180.673190723061No Hit
TTCAAGTAATTCAGGATAGGTT541320.6281009379974845No Hit
TGTAAACATCCCCGACTGGAAGCT540850.6275555906228099No Hit
TATTGCACTTGTCCCGGCCTGT490290.5688901368705879No Hit
GCCTGTCTGAGCGTCGCT462520.536668229222265No Hit
TTCACAGTGGCTAAGTTCCG385970.44784622596410456No Hit
CGCCTGTCTGAGCGTCGCT385340.447115228419328No Hit
CAAAGAATTCTCCTTTTGGGCT372160.43182229565717833No Hit
TAGCTTATCAGACTGATGTTGACA364960.4234680380025898No Hit
ACTGGACTTGGAGTCAGAAGGC357360.41464965492274636No Hit
TGTAAACATCCTACACTCTCAGCT329100.3818591936284862No Hit
CAACGGAATCCCAAAAGCAGCTGT310290.3600336955058735No Hit
AACATTCAACGCTGTCGGTGAGTT291210.3378949127212138No Hit
TGAGGTAGTAGTTTGTACAGTT287900.3340542748272293No Hit
CCCATAAAGTAGAAAGCACTAC281490.32661666488751917No Hit
TTCTCACTACTGCACTTGACTAG255310.29623965580458467No Hit
AACCGGGCGGAAACACCA236080.2739268259854543No Hit
TGTCAGTTTGTCAAATACCCCAA229640.26645440663885006No Hit
CATTGCACTTGTCTCGGTCTGA203910.2365995386593273No Hit
CTTCTCACTACTGCACTTGACTAG198030.22977689490807995No Hit
TAGCAGCACGTAAATATTGGC183040.21238379459665183No Hit
CAACACCAGTCGATGGGCTGT182110.2113047029829341No Hit
CAACGGAATCCCAAAAGCAGCT181510.21060851484505172No Hit
TCCTGTCTGAGCGTCGCT178670.20731322432574179No Hit
TACGCCTGTCTGAGCGTCGCT178090.20664024245912216No Hit
TGAGGTAGTAGTTTGTGCTGTT175910.20411075889148284No Hit
TTAATGCTAATCGTGATAGGGGTTT171130.1985644600596865No Hit
CAAAGAATTCTCCTTTTGGGCTT168650.19568688242310603No Hit
CCCCCACTGCTAAATTTGACTGGCTT168470.1954780259817413No Hit
CCCATAAAGTAGAAAGCACT168370.19536199462542755No Hit
TGTCAGTTTGTCAAATACCCCA164830.19125448461192152No Hit
TGAGAACTGAATTCCATAGGCTGT160950.1867524679869488No Hit
TTATAAAGCAATGAGACTGATT158940.18442023772504282No Hit
ACTGGACTTGGAGTCAGAAGGCA154730.17953531762423477No Hit
TTCACAGTGGCTAAGTTCTG151710.1760311706635601No Hit
TTCAAGTAATCCAGGATAGGCTT146510.16999754013524615No Hit
CTACGCCTGTCTGAGCGTCGCT146480.16996273072835202No Hit
CACTAGATTGTGAGCTCCTGGAA143460.1664585837676774No Hit
ACCCCCCACTGCTAAATTTGACTG143320.16629613986883815No Hit
ATCACATTGCCAGGGATTTCCA140350.16285000858632037No Hit
TGTAAACATCCTACACTCAGCT139500.16186374205765366No Hit
CCCCCCACTGCTAAATTTGACTGG137830.15992601840721438No Hit
TTCAAGTAATCCAGGATAGGC132690.15396200669268864No Hit
TAGCAGCACGTAAATATTGGCGT129630.15041144718948848No Hit
AGCAGCATTGTACAGGGCTATGT123560.14336834386124506No Hit
TATTGCACTTGTCCCGGCCTGTT122690.14235887106131562No Hit
ACTGGACTTGGAGTCAGAAGG118410.13739272901108796No Hit
AACGGAATCCCAAAAGCAGCTG118090.13702142867088402No Hit
CACTAGATTGTGAGCTCCTGGA116280.1349212611216055No Hit
TGTAAACATCCCCGACTGGAAGC115070.13351728171020935No Hit
CCCCCCACTGCTAAATTTGACTGTA113090.13121986085519752No Hit
GCGTTCGCGCTTTCCCCTG108330.12569676829466395No Hit
TATTGCACTTGTCCCGGCCTGTAG104930.12175170217999713No Hit
ATGCACCTGGGCAAGGATTCTGA104560.12132238616163632No Hit
TGAGGTAGTAGGTTGTATAGT103470.12005764437781666No Hit
ACTGGACTTGGAGTCAGAAGGCT102120.1184912210675813No Hit
AGCTCGGTCTGAGGCCCCTCAGT101960.11830557089747933No Hit
CCCCCCACTGCTAAATTTGACTGT100170.11622860961946356No Hit
TTTAAACATCCTTGACTGGAAGCT100070.11611257826314983No Hit
ATCACATTGCCAGGGATTTCC99140.11503348664943214No Hit
CGCGACCTCAGATCAGACGTGGCG97980.11368752291619287No Hit
AGCTACATTGTCTGCTGGGTTT97590.11323500062656933No Hit
ACGCCTGTCTGAGCGTCGCT97540.11317698494841247No Hit
CAAAGTGCTGTTCGTGCAGGTAG97410.11302614418520461No Hit
AGCAGCATTGTACAGGGCTATG97010.11256201875994969No Hit
CACCCGTAGAACCGACCTTGCG95380.11067070765203588No Hit
TTAATGCTAATCGTGATAGGGGT94930.1101485665486241No Hit
TATTGCACTTGTCCCGGCCTGTAAA93140.10807160527060833No Hit
CTCCCACTGCTTCACTTGACTAG92780.1076538923878789No Hit
TGTAAACATCCTTGACTGGAAGC91450.10611067534890628No Hit
CCAAGTCCTTCTGATCGAGGCC90980.10556532797423175No Hit
TATTGCACTTGTCCCGGCCTGTAAT88200.10233965626871004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)