FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7279078
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG115027515.802482127544176No Hit
CCCCCCACTGCTAAATTTGACTG6705839.212471689409016No Hit
TTCAAGTAATCCAGGATAGGCT2850993.916691097416459No Hit
CCCCCACTGCTAAATTTGACTG2631813.615581533815134No Hit
TAGCTTATCAGACTGATGTTGAC1936282.6600621672140345No Hit
TAGCAGCACGTAAATATTGGCG1817622.497047016119349No Hit
AACATTCAACGCTGTCGGTGAG1776872.4410646513198513No Hit
TGAGGTAGTAGATTGTATAGTT1730372.3771829344320805No Hit
TAGCTTATCAGACTGATGTTGA1434021.9700571968043206No Hit
AAGCTGCCAGTTGAAGAACTGT1396171.9180588530580382No Hit
TAGCTTATCAGACTGATGTTGACT1295061.7791538983371245No Hit
TGAGGTAGTAGGTTGTATAGTT883241.2133954327732166No Hit
TCGTACGACTCTTAGCGG829381.139402545212457No Hit
TATTGCACTTGTCCCGGCCTGTAA670010.9204599813328006No Hit
TGTAAACATCCTTGACTGGAAGCT589610.8100064321333004No Hit
TATTGCACTTGTCCCGGCCTGTAT562840.773229796411029No Hit
AACATTCAACGCTGTCGGTGAGT550830.7567304540492629No Hit
TATTGCACTTGTCCCGGCCTGT550300.7560023398567786No Hit
TTCAAGTAATTCAGGATAGGTT522620.7179755458040153No Hit
TGAGGTAGTAGTTTGTACAGTT496530.6821330943287048No Hit
CATAAAGTAGAAAGCACTACT494580.6794541836205079No Hit
CAACGGAATCCCAAAAGCAGCTGT478990.6580366359585651No Hit
AGCAGCATTGTACAGGGCTATGA424350.5829721841145266No Hit
TATTGCACTTGTCCCGGCCTGTA407250.5594801979041851No Hit
TTCTCACTACTGCACTTGACTAG399820.5492728612057736No Hit
CAACGGAATCCCAAAAGCAGCT395340.5431182355787368No Hit
TGATGTAGTAGATTGTATAGTT392470.5391754285364163No Hit
TAGCTTATCAGACTGATGTTGACA309390.42504009436359935No Hit
TGTAAACATCCCCGACTGGAAGCT304100.41777269044238846No Hit
TGAGAACTGAATTCCATGGGTT298240.40972222031416616No Hit
CCCATAAAGTAGAAAGCACTAC280670.38558454793313107No Hit
CAAAGAATTCTCCTTTTGGGCT277900.3817791209271284No Hit
CATTGCACTTGTCTCGGTCTGA270270.3712970241560813No Hit
TCGTTTCCCGGCCAATGCACCA252840.34735168382589116No Hit
TGAGGTAGTAGTTTGTGCTGTT242160.3326794959471515No Hit
CCCCCACTGCTAAATTTGACTGGCTT238190.3272255085053354No Hit
CTTCTCACTACTGCACTTGACTAG226870.311674088394162No Hit
CCCATAAAGTAGAAAGCACT225070.30920124774044183No Hit
AACGGAATCCCAAAAGCAGCTG218710.30046387743063063No Hit
TAGCAGCACGTAAATATTGGC202700.27846933361615306No Hit
TGATGTAGTAGGTTGTATAGTT199600.2742105524903017No Hit
ACTGGACTTGGAGTCAGAAGGC196930.27054250552061676No Hit
TTCACAGTGGCTAAGTTCCGC180410.24784732352091843No Hit
CCCATAAAGTAGAAAGCACTA179570.24669333121584905No Hit
CTCCCACTGCTTCACTTGACTAG178340.24500355676914026No Hit
CACCCGGCTGTGTGCACATGTGC178040.2445914166601869No Hit
CAACACCAGTCGATGGGCTGT169910.2334224197075509No Hit
TGAGGTAGTAGATTGTATAGT163520.22464383538684432No Hit
CACCCGTAGAACCGACCTTGCG163270.22430038529604987No Hit
ACCCCCCACTGCTAAATTTGACTG162470.2232013450055076No Hit
TGTAAACATCCTACACTCTCAGCT155920.21420295262669256No Hit
TTCACAGTGGCTAAGTTCCG146340.2010419451474486No Hit
TTCACAGTGGCTAAGTTCTG140530.19306016503738524No Hit
TGTCAGTTTGTCAAATACCCCA138840.19073844242361465No Hit
GCCTGTCTGAGCGTCGCT135630.1863285432578137No Hit
AATCCTTGGAACCTAGGTGTGAGT131820.18109436387410602No Hit
TGAGAACTGAATTCCATAGGCTGT129660.17812695508964185No Hit
TGAGGTAGTAAGTTGTATTGTT124790.17143654732096567No Hit
ATGCACCTGGGCAAGGATTCTGA123560.16974677287425688No Hit
AATGCACCCGGGCAAGGATTCT119860.16466371153049877No Hit
CACTAGATTGTGAGCTCCTGGAA117130.1609132365390232No Hit
ATCACATTGCCAGGGATTTCC115800.15908608205599664No Hit
TTCAAGTAATCCAGGATAGGC114750.15764359167465988No Hit
CACTAGATTGTGAGCTCCTGGA114470.15725892757297008No Hit
TGATGTAGTAGTTTGTACAGTT113690.15618736328969135No Hit
CAAAGAATTCTCCTTTTGGGC113210.155527939115366No Hit
TGAGGTAGTAGATTGTATAGTTA112250.1542090907667152No Hit
TTCACAGTGGCTAAGTTCTGC106590.14643338071112852No Hit
TGTAAACATCCCCGACTGGA105760.14529312640969091No Hit
TATTGCACTTGTCCCGGCCTGTT104510.14357587595571858No Hit
CTCCCACTGCTTCACTTGACTAGCCT103970.14283402375960252No Hit
CAAAGAATTCTCCTTTTGGGCTT99510.13670687413982924No Hit
CAAAGTGCTGTTCGTGCAGGTAG97950.13456374557327178No Hit
TCGTTTCCCGGCCAACGCACCA97850.134426365536954No Hit
CCCCCCACTGCTAAATTTGACTGG97440.13386310738805107No Hit
TGAGGTAGTAGTTTGTGCTGTTA94780.13020879842199795No Hit
TTAATGCTAATCGTGATAGGGGTT92370.12689793954673928No Hit
TAGCAGCACATCATGGTTTACA89670.12318867856615906No Hit
TTCAAGTAATCCAGGATAGGCTT87990.12088069395602026No Hit
ACTGGACTTGGAGTCAGAAGGCT83960.11534427849241345No Hit
AGCTCGGTCTGAGGCCCCTCAGT82500.11333852996217378No Hit
AGCAGCATTGTACAGGGCTATGT81580.11207463362805015No Hit
TGTAAACATCCCCGACTGGAAGC79120.1086950847346326No Hit
TTATAAAGCAATGAGACTGATT78930.10843406266562881No Hit
TATTGCACTTGTCCCGGCCTGTAG75140.1032273592891847No Hit
ACTGGACTTGGAGTCAGAAGGCA74220.10196346295506106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)