FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7212426
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT6702749.293322385560696No Hit
CAACGGAATCCCAAAAGCAGCTG5479937.597901177772916No Hit
CCCCCCACTGCTAAATTTGACTG3607185.001340741658909No Hit
AAGCTGCCAGTTGAAGAACTGT3206974.446451166362053No Hit
TTCAAGTAATCCAGGATAGGCT2510783.4811864967488053No Hit
AACATTCAACGCTGTCGGTGAGT2290213.175367067890887No Hit
TAGCAGCACGTAAATATTGGCG2080382.8844386063718366No Hit
AACATTCAACGCTGTCGGTGAG1612032.235073191738813No Hit
TCCTGTCTGAGCGTCGCT1608892.230719594211435No Hit
TAGCTTATCAGACTGATGTTGAC1478472.049892782262168No Hit
TAGCTTATCAGACTGATGTTGA1447792.0073550841284193No Hit
CCCCCACTGCTAAATTTGACTG1328581.8420708926510998No Hit
TGAGGTAGTAGGTTGTATAGTT1186071.6444813437253982No Hit
TAGCTTATCAGACTGATGTTGACT1142751.5844183357999098No Hit
CATAAAGTAGAAAGCACTACT911421.2636802096825672No Hit
AACCGGGCGGAAACACCA700190.9708106537245581No Hit
TGAGGTAGTAGATTGTATAGTT690060.9567654489626651No Hit
TGTAAACATCCTTGACTGGAAGCT590440.8186427146704867No Hit
TATTGCACTTGTCCCGGCCTGTAA579340.8032526087616012No Hit
TATTGCACTTGTCCCGGCCTGT545880.756860451670492No Hit
TATTGCACTTGTCCCGGCCTGTAT505000.7001804940529026No Hit
CGCCTGTCTGAGCGTCGCT451090.6254344931927205No Hit
TTCAAGTAATTCAGGATAGGTT436920.6057878444784044No Hit
CAACACCAGTCGATGGGCTGT411130.5700301119207324No Hit
AGCAGCATTGTACAGGGCTATGA383010.5310418436182223No Hit
TCGTACGACTCTTAGCGG361020.5005527959662948No Hit
CCCATAAAGTAGAAAGCACT353550.49019567063842323No Hit
CAACGGAATCCCAAAAGCAGCTGT331010.45894405017119067No Hit
ACTGGACTTGGAGTCAGAAGGC307310.4260840943116782No Hit
TATTGCACTTGTCCCGGCCTGTA294550.4083924049965989No Hit
TGAGGTAGTAGTTTGTACAGTT294340.4081012408307551No Hit
CATTGCACTTGTCTCGGTCTGA269340.3734388401350669No Hit
CAACGGAATCCCAAAAGCAGCT267660.37110952680831666No Hit
TGTAAACATCCCCGACTGGAAGCT258380.3582428436700772No Hit
CCCATAAAGTAGAAAGCACTAC242660.3364471261126284No Hit
CACCCGGCTGTGTGCACATGTGC224570.3113654129692284No Hit
TGTAAACATCCTACACTCTCAGCT186980.25924702728319154No Hit
GGAATACCGGGTGCTGTAGGCTTT183580.2545329407885779No Hit
CCCTGTCTGAGCGTCGCT181320.25139945976568767No Hit
AACGGAATCCCAAAAGCAGCTG175140.2428309143137136No Hit
TGAGGTAGTAGTTTGTGCTGTT169850.23549635032650595No Hit
TTCACAGTGGCTAAGTTCCG167700.23251538386667675No Hit
TAGCTTATCAGACTGATGTTGACA157810.21880293815146248No Hit
TAGCAGCACGTAAATATTGGC152130.21092764071340211No Hit
GGAATACCGGGTGCTGTAGGCTT149790.2076832400082857No Hit
AACATTCAACGCTGTCGGTGAGTT146620.20328804760007244No Hit
TGTCAGTTTGTCAAATACCCCAA144730.2006675701074784No Hit
TTATAAAGCAATGAGACTGATT135950.18849413498315268No Hit
TTCACAGTGGCTAAGTTCCGC135080.18728788343894273No Hit
TTCACAGTGGCTAAGTTCTG130660.18115957099594504No Hit
TTCAAGTAATCCAGGATAGGC130030.18028607849841372No Hit
TTCACAGTGGCTAAGTTCTGC127590.17690302819051454No Hit
TTCTCACTACTGCACTTGACTAG125240.17364476252511984No Hit
TGAGGTAGTAGGTTGTATAGT123200.17081631062835168No Hit
TTCAAGTAATCCAGGATAGGCTT116920.16210911557359478No Hit
CTTCTCACTACTGCACTTGACTAG114990.15943317823988767No Hit
TACGCCTGTCTGAGCGTCGCT109770.15219566897462794No Hit
TGTCAGTTTGTCAAATACCCCA109710.1521124792129583No Hit
TGATGTAGTAGGTTGTATAGTT109010.15114193199347903No Hit
ATCCCGGACGAGCCCCCA103880.1440292073707238No Hit
TAGCTTATCAGACTGATGTTG102630.1422960873359394No Hit
TCGTTTCCCGGCCAATGCACCA102330.14188013852759113No Hit
TGAGGTAGTAGGTTGTGTGGTT101850.1412146204342339No Hit
CTCCCACTGCTTCACTTGACTAG98760.13693034770824686No Hit
ACCGGGTGCTGTAGGCTT98210.1361677748929417No Hit
CTACGCCTGTCTGAGCGTCGCT96260.13346410763867803No Hit
CAAAGAATTCTCCTTTTGGGCT95390.13225785609446808No Hit
TGTAAACATCCTACACTCAGCT95390.13225785609446808No Hit
AACATTCAACGCTGTCGGTGA94420.13091295494747537No Hit
ACTGGACTTGGAGTCAGAAGG93910.13020584197328333No Hit
CACTAGATTGTGAGCTCCTGGA93230.12926302467436063No Hit
ACGCCTGTCTGAGCGTCGCT92900.12880548098517752No Hit
ATCCCACCGCTGCCACCA91660.1270862259106714No Hit
AATGACACGATCACTCCCGTTG91220.12647616765842726No Hit
ACCACAGGGTAGAACCACGGAC89300.12381409528499843No Hit
AGCTCGGTCTGAGGCCCCTCAGT89000.12339814647665016No Hit
TCGAGGAGCTCACAGTCTAGT88610.12285741302579742No Hit
AATGCACCCGGGCAAGGATTCT87830.12177594612409195No Hit
TAGCAGCACGTAAATATTGGCGT86780.12032012529487304No Hit
CAACGGAATCCCAAAAGCAGCTGA85650.11875338478342792No Hit
AGCAGCATTGTACAGGGCTATGT85460.1184899505381407No Hit
CCCATAAAGTAGAAAGCACTA83680.1160219876086077No Hit
CAAAGTGCTGTTCGTGCAGGT82290.11409475812992743No Hit
GCGTTCGCGCTTTCCCCTG81780.1133876451557354No Hit
TATTGCACTTGTCCCGGCCTGTT81000.11230617825402991No Hit
TGAGGTAGTAGATTGTATAGT80020.11094741214675895No Hit
ACTGGACTTGGAGTCAGAAGGCA77830.10791098584581665No Hit
ACCGGGTGCTGTAGGCTTT77810.10788325592526012No Hit
AGCAGCATTGTACAGGGCTATG76760.1064274350960412No Hit
TGTAAACATCCCCGACTGGAAGC76300.10578964692324053No Hit
CCCCCCACTGCTAAATTTGACTGG75470.10463885522014368No Hit
GCCTGTCTGAGCGTCGCA75140.1041813115309606No Hit
TGAGGTAGTAAGTTGTATTGTT73270.10158856395892311No Hit
CAAAGAATTCTCCTTTTGGGCTT72350.10031298761332177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)