FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8011770
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT132317616.515401715226474No Hit
CAACGGAATCCCAAAAGCAGCTG3955094.936599527944511No Hit
AAGCTGCCAGTTGAAGAACTGT3486704.351972160958191No Hit
TTCAAGTAATCCAGGATAGGCT3439364.292884094276296No Hit
TAGCAGCACGTAAATATTGGCG2922033.6471715987852873No Hit
CATAAAGTAGAAAGCACTACT2524023.1503899887290827No Hit
CCCCCCACTGCTAAATTTGACTG2344802.9266941013034575No Hit
TAGCTTATCAGACTGATGTTGA2171122.7099130404392535No Hit
AACCGGGCGGAAACACCA2155262.690117165120816No Hit
TGAGGTAGTAGGTTGTATAGTT1580711.9729847461921648No Hit
TGAGGTAGTAGATTGTATAGTT1281621.5996714833301506No Hit
TAGCTTATCAGACTGATGTTGAC1208061.5078565660272325No Hit
TAGCTTATCAGACTGATGTTGACT1083491.3523728214863882No Hit
AACATTCAACGCTGTCGGTGAGT1073031.3393170298198775No Hit
TTCAAGTAATTCAGGATAGGTT978071.2207914106371No Hit
AACATTCAACGCTGTCGGTGAG948201.1835087627328293No Hit
CCCCCACTGCTAAATTTGACTG947001.1820109663657346No Hit
TGTAAACATCCTTGACTGGAAGCT620900.7749848036076922No Hit
TATTGCACTTGTCCCGGCCTGT533890.6663820853569187No Hit
TGAGGTAGTAGTTTGTACAGTT517070.645387972944805No Hit
TATTGCACTTGTCCCGGCCTGTAA436950.5453851021684347No Hit
TATTGCACTTGTCCCGGCCTGTAT419220.5232551608446074No Hit
TTCACAGTGGCTAAGTTCCG349580.4363330450075326No Hit
CAACACCAGTCGATGGGCTGT336960.42058121988025116No Hit
TCGTACGACTCTTAGCGG336760.42033158715240204No Hit
TGAGAACTGAATTCCATGGGTT328250.4097097145824206No Hit
CATTGCACTTGTCTCGGTCTGA289640.36151811647114185No Hit
CAACGGAATCCCAAAAGCAGCTGT280760.3504344233546395No Hit
TTCACAGTGGCTAAGTTCCGC270770.33796526859857434No Hit
TGTCAGTTTGTCAAATACC260320.3249219585684561No Hit
AGCAGCATTGTACAGGGCTATGA253620.31655926218550956No Hit
TATTGCACTTGTCCCGGCCTGTA235430.2938551655876292No Hit
TGATGTAGTAGGTTGTATAGTT228050.2846437179299955No Hit
TTCTCACTACTGCACTTGACTAG227790.2843191953837916No Hit
TGAGGTAGTAGTTTGTGCTGTT222510.277728891368574No Hit
TTATAAAGCAATGAGACTGATT221440.276393356274581No Hit
CGCCTGTCTGAGCGTCGCT218080.27219952644671525No Hit
CCCATAAAGTAGAAAGCACTAC212990.26584637352295437No Hit
TTCAAGTAATCCAGGATAGGC210370.26257618478813044No Hit
TAGCTTATCAGACTGATGTTG190440.23770028345796246No Hit
TAGCAGCACGTAAATATTGGC187700.23428031508642908No Hit
TGTAAACATCCTACACTCTCAGCT185300.23128472235223926No Hit
TGATGTAGTAGATTGTATAGTT184480.23026122816805777No Hit
TTAATGCTAATCGTGATAGGGGTT183670.22925021562026868No Hit
TGAGGTAGTAGGTTGTATAGT183100.22853876234589862No Hit
TTCACAGTGGCTAAGTTCTGC176110.21981409850757075No Hit
ATCCCGGACGAGCCCCCA174840.2182289306857286No Hit
TTCACAGTGGCTAAGTTCTG172410.21519589304236142No Hit
TGTAAACATCCCCGACTGGAAGCT169580.2116635899432959No Hit
TGAGGTAGTAGATTGTATAGT162320.20260192192237172No Hit
ACTGGACTTGGAGTCAGAAGGC157620.19673555281791663No Hit
GCCTGTCTGAGCGTCGCA154930.19337799262834554No Hit
CTCCCACTGCTTCACTTGACTAG142030.17727668168207525No Hit
TAGTGGGTTATCAGAACT138260.17257110476211873No Hit
CACCCGGCTGTGTGCACATGTGC130030.16229871801112614No Hit
TGTCAGTTTGTCAAATACCCCA129940.162186383283594No Hit
TGAGGTAGTAAGTTGTATTGTT129670.16184937910099767No Hit
GCCTGTCTGAGCGTCGCG125580.1567443898164825No Hit
TAGCTTATCAGACTGATGTTGACA123800.15452265853862507No Hit
TTCAAGTAATCCAGGATAGGCTT120360.15022897561961962No Hit
TAGCAGCACATAATGGTTTGT119590.1492678896174004No Hit
AACGGAATCCCAAAAGCAGCTG117780.1470087134303656No Hit
TGTAAACATCCTACACTCAGCT117390.14652192961105973No Hit
CCCATAAAGTAGAAAGCACT116490.14539858233573855No Hit
CTTCTCACTACTGCACTTGACTAG111660.13936995195818153No Hit
TGTCAGTTTGTCAAATACCC111210.13880827832052092No Hit
TGTCAGTTTGTCAAATACCCCAA109680.13689858795247492No Hit
TGTAAACATCCCCGACTGGA109350.13648669395152382No Hit
CAACGGAATCCCAAAAGCAGCT108490.13541327322177246No Hit
ACCGGGTGCTGTAGGCTT107000.13355350939929628No Hit
TTCACAGTGGCTAAGTTCCGCT105960.1322554192144807No Hit
CAAAGAATTCTCCTTTTGGGCT104050.12987142666352128No Hit
TGTCAGTTTGTCAAATACCCC98770.12328112264830368No Hit
TCGAGGAGCTCACAGTCTAGT98230.12260711428311098No Hit
CTACGCCTGTCTGAGCGTCGCT98210.12258215101032606No Hit
AATGACACGATCACTCCCGTTG97750.12200799573627301No Hit
CATAAAGTAGAAAGCACTAC97160.121271579189118No Hit
GCCTGTCTGAGCGTCGCTT97020.1210968362796236No Hit
CAAAGTGCTGTTCGTGCAGGT95350.11901240300208318No Hit
TCCTGTCTGAGCGTCGCT95220.11885014172898123No Hit
TGAGAACTGAATTCCATAGGCTGT91620.1143567526276965No Hit
TTCAAGTAATTCAGGATAGGT90170.11254691535079016No Hit
TCGACCGGACCTCGACCGGCT85760.10704251370171636No Hit
TAGCAGCACGTAAATATTGGCGT84940.10601901951753483No Hit
GCCGGTCTGAGCGTCGCT84270.10518274987924017No Hit
AGCTCGGTCTGAGGCCCCTCAGT82810.10336043096594136No Hit
GGAATACCGGGTGCTGTAGGCTTT82430.10288612878302797No Hit
ACCGGGTGCTGTAGGCTTT81300.10147570387068025No Hit
ATCACATTGCCAGGGATTTCC81300.10147570387068025No Hit
ATAAAGTAGAAAGCACTACT80310.10024002186782696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)