FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11422428
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10256828.979544454121314No Hit
CCCCCCACTGCTAAATTTGACTG6797395.9509151644466485No Hit
GCCTGTCTGAGCGTCGCT5161174.5184526442188995No Hit
TTCAAGTAATCCAGGATAGGCT4686094.102534067187817No Hit
AAGCTGCCAGTTGAAGAACTGT4305213.769084821545822No Hit
AACATTCAACGCTGTCGGTGAGT4269383.7377167096172546No Hit
AACATTCAACGCTGTCGGTGAG3133142.7429719845903167No Hit
TAGCAGCACGTAAATATTGGCG3015612.640077923887986No Hit
CCCCCACTGCTAAATTTGACTG2221121.9445252795640295No Hit
TAGCTTATCAGACTGATGTTGA1889741.6544118290787213No Hit
TAGCTTATCAGACTGATGTTGAC1829271.6014721213388257No Hit
TAGCTTATCAGACTGATGTTGACT1780901.5591256079705647No Hit
TATTGCACTTGTCCCGGCCTGTAA1709141.4963018370525076No Hit
TGAGGTAGTAGGTTGTATAGTT1639501.4353340638260097No Hit
TATTGCACTTGTCCCGGCCTGTAT1466961.2842803649101575No Hit
TATTGCACTTGTCCCGGCCTGT1284531.1245682616690602No Hit
CATAAAGTAGAAAGCACTACT1106600.96879577616948No Hit
TGAGGTAGTAGATTGTATAGTT976000.8544593146045656No Hit
TTCAAGTAATTCAGGATAGGTT913480.7997248921157569No Hit
TGTAAACATCCTTGACTGGAAGCT897880.7860675506118313No Hit
TCGTACGACTCTTAGCGG868040.7599435076325278No Hit
CAACGGAATCCCAAAAGCAGCT804340.7041760298248324No Hit
CAACGGAATCCCAAAAGCAGCTGT778460.6815188504580637No Hit
TCCTGTCTGAGCGTCGCT717550.6281939356501087No Hit
TTAATGCTAATCGTGATAGGGGTT712910.6241317520233002No Hit
TATTGCACTTGTCCCGGCCTGTA684380.5991545755420826No Hit
AACCGGGCGGAAACACCA621800.5443676248167203No Hit
AGCAGCATTGTACAGGGCTATGA581810.5093575551537729No Hit
GGAATACCGGGTGCTGTAGGCTTT568860.49802021076429637No Hit
CCCATAAAGTAGAAAGCACTAC562960.49285493416986303No Hit
CCCATAAAGTAGAAAGCACT524790.45943822101570697No Hit
TGTAAACATCCCCGACTGGAAGCT500740.43838315286382196No Hit
CACCCGGCTGTGTGCACATGTGC472910.41401880580906264No Hit
TGAGAACTGAATTCCATGGGTT464210.4064022115087966No Hit
ACTGGACTTGGAGTCAGAAGGC443690.38843755460747925No Hit
TCGTTTCCCGGCCAATGCACCA429950.37640858843671415No Hit
TGAGGTAGTAGTTTGTACAGTT428440.3750866278167829No Hit
CATTGCACTTGTCTCGGTCTGA414400.36279502046325No Hit
GGAATACCGGGTGCTGTAGGCTT374060.3274785360870736No Hit
TGTAAACATCCTACACTCTCAGCT372780.3263579337072643No Hit
CGCCTGTCTGAGCGTCGCT348210.3048476208385818No Hit
CAACACCAGTCGATGGGCTGT341950.2993671748248271No Hit
CACCCGTAGAACCGACCTTGCG300570.26314020101505564No Hit
AACGGAATCCCAAAAGCAGCTG290960.2547269284603939No Hit
AGCTCGGTCTGAGGCCCCTCAGT273260.23923109867709388No Hit
TGAGAACTGAATTCCATAGGCTGT268380.23495880210407105No Hit
TAGCTTATCAGACTGATGTTGACA254830.2230961753490589No Hit
AACATTCAACGCTGTCGGTGAGTT253710.22211564826672578No Hit
TGAGGTAGTAGTTTGTGCTGTT245220.2146829027943971No Hit
CAACGGAATCCCAAAAGCAGCTGA238690.2089660797161514No Hit
TTCAAGTAATCCAGGATAGGC232520.20356442605722705No Hit
TGAGGTAGTAGGTTGTGTGGTT215340.18852384099072456No Hit
TTCTCACTACTGCACTTGACTAG214040.18738572919873078No Hit
TTCAAGTAATCCAGGATAGGCTT214000.18735071037436174No Hit
CTCCCACTGCTTCACTTGACTAG206660.18092475610264297No Hit
TATTGCACTTGTCCCGGCCTGTT203460.1781232501531198No Hit
TAGCAGCACGTAAATATTGGC200730.17573321538993286No Hit
ATCCCGGACGAGCCCCCA196330.17188114470933852No Hit
TTCACAGTGGCTAAGTTCTGC194010.16985005289593422No Hit
CACTAGATTGTGAGCTCCTGGA190700.1669522451793962No Hit
ACCGGGTGCTGTAGGCTT185320.16224221330176036No Hit
CTACGCCTGTCTGAGCGTCGCT185070.16202334564945386No Hit
TTCACAGTGGCTAAGTTCCG180200.1577598037825233No Hit
TATTGCACTTGTCCCGGCCTGTAG177030.1549845619512769No Hit
CCCCCACTGCTAAATTTGACTGGCTT174930.15314607367190233No Hit
CAAAGAATTCTCCTTTTGGGCT168790.14777068413125474No Hit
AATGCACCCGGGCAAGGATTCT164910.1443738581674579No Hit
CACTAGATTGTGAGCTCCTGGAA162140.14194880457990194No Hit
ACCGGGTGCTGTAGGCTTA157600.13797416801401594No Hit
TTCACAGTGGCTAAGTTCCGC156150.13670473563063826No Hit
TTCACAGTGGCTAAGTTCTG155920.13650337739051627No Hit
CCCATAAAGTAGAAAGCACTA154240.1350325867670166No Hit
ACCGGGTGCTGTAGGCTTT152920.1338769655628383No Hit
TGAGGTAGTAGGTTGTATAGT152010.13308028730844265No Hit
TCGTTTCCCGGGCGGCGCACCA150110.13141689315091326No Hit
TGATGTAGTAGGTTGTATAGTT149940.13126806314734485No Hit
TGTAAACATCCCCGACTGGAAGC139880.1224608288185314No Hit
AGCAGCATTGTACAGGGCTATGT139730.12232950822714751No Hit
CAAAGTGCTGTTCGTGCAGGTAG138060.1208674723097401No Hit
TTATAAAGCAATGAGACTGATT135180.11834611695516926No Hit
CCCCCCACTGCTAAATTTGACTGG131640.11524695099850925No Hit
TATTGCACTTGTCCCGGCCTGTAAT130870.11457283862940525No Hit
CTTCTCACTACTGCACTTGACTAG130780.11449404627457489No Hit
TCGAGGAGCTCACAGTCTAGT130560.11430144274054518No Hit
AATGACACGATCACTCCCGTTG128350.11236665269415574No Hit
ACCCCCCACTGCTAAATTTGACTG126430.11068574912444183No Hit
TGTAAACATCCTACACTCAGCT126290.1105631832391502No Hit
AACATTCAACGCTGTCGGTGA124300.10882099672679048No Hit
AGAGGTAGTAGGTTGCATAGTT123760.10834824259780845No Hit
TATTGCACTTGTCCCGGCCTGA123350.1079892996480258No Hit
CAAAGTGCTGTTCGTGCAGGT122200.10698250844741591No Hit
TATTGCACTTGTCCCGGCCTGTAAA120980.1059144343041602No Hit
CCCCCCACTGCTAAATTTGACTGGCT119900.10496892604619613No Hit
GAATACCGGGTGCTGTAGGCTTT119580.10468877545124382No Hit
TACGCCTGTCTGAGCGTCGCT119140.10430356838318439No Hit
TGTCAGTTTGTCAAATACCCCA119040.10421602132226178No Hit
ATCACATTGCCAGGGATTTCC118220.10349813542269648No Hit
ACCACAGGGTAGAACCACGGAC117900.10321798482774416No Hit
TATTGCACTTGTCCCGGCCTGTTT116570.10205360891747357No Hit
CAAAGAATTCTCCTTTTGGGCTT116380.10188726950172064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)