FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10698197
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT208223819.463447906221955No Hit
CAACGGAATCCCAAAAGCAGCTG8410917.8619883331742715No Hit
CCCCCCACTGCTAAATTTGACTG5139854.804407695988399No Hit
AACATTCAACGCTGTCGGTGAGT3461443.235535857116858No Hit
TTCAAGTAATCCAGGATAGGCT3383313.162504859463702No Hit
AAGCTGCCAGTTGAAGAACTGT2762982.5826594892578627No Hit
TAGCAGCACGTAAATATTGGCG2719182.5417180109882067No Hit
AACATTCAACGCTGTCGGTGAG2691812.5161342607544057No Hit
CCCCCACTGCTAAATTTGACTG1867261.7453969112739276No Hit
TGAGGTAGTAGGTTGTATAGTT1472141.3760636488559708No Hit
TAGCTTATCAGACTGATGTTGA1465161.3695391849673362No Hit
TAGCTTATCAGACTGATGTTGACT1324031.2376197596660448No Hit
TAGCTTATCAGACTGATGTTGAC1239731.1588214350511585No Hit
TCGTACGACTCTTAGCGG1057320.9883160685861364No Hit
CATAAAGTAGAAAGCACTACT1043860.9757345092822651No Hit
TATTGCACTTGTCCCGGCCTGTAA982570.9184444818131503No Hit
TATTGCACTTGTCCCGGCCTGT849460.7940216468251613No Hit
TATTGCACTTGTCCCGGCCTGTAT789810.7382645879487918No Hit
TGTAAACATCCTTGACTGGAAGCT679490.6351444079782789No Hit
TGAGGTAGTAGATTGTATAGTT656520.6136735003103794No Hit
AACCGGGCGGAAACACCA635280.5938196875604366No Hit
TTAATGCTAATCGTGATAGGGGTT585860.5476249876497881No Hit
TATTGCACTTGTCCCGGCCTGTA546790.5111048151384762No Hit
CAACGGAATCCCAAAAGCAGCTGT543060.5076182463269279No Hit
CGCCTGTCTGAGCGTCGCT494420.46215264123478No Hit
TTCAAGTAATTCAGGATAGGTT477790.4466079658095658No Hit
CCCATAAAGTAGAAAGCACTAC476150.44507499721682076No Hit
CAACACCAGTCGATGGGCTGT468170.43761579638139025No Hit
CCCATAAAGTAGAAAGCACT457150.4273149952277005No Hit
AGCAGCATTGTACAGGGCTATGA445200.4161448887134907No Hit
TGTAAACATCCCCGACTGGAAGCT411080.38425166408881795No Hit
CATTGCACTTGTCTCGGTCTGA338130.31606260382006424No Hit
ACTGGACTTGGAGTCAGAAGGC337030.3150343931785889No Hit
TGAGAACTGAATTCCATGGGTT311660.29132011683837944No Hit
TGAGGTAGTAGTTTGTACAGTT304930.2850293371864437No Hit
TGAGAACTGAATTCCATAGGCTGT290980.27198975677864223No Hit
ATCCCGGACGAGCCCCCA284160.2656148508014949No Hit
TTCACAGTGGCTAAGTTCCG284030.26549333499841143No Hit
CAACGGAATCCCAAAAGCAGCT273810.25594032340215833No Hit
AACATTCAACGCTGTCGGTGAGTT273730.25586554444641463No Hit
TGTCAGTTTGTCAAATACCCCAA252610.23612390013008733No Hit
CACCCGGCTGTGTGCACATGTGC249080.23282427870789818No Hit
TGTAAACATCCTACACTCTCAGCT240050.22438360407833208No Hit
TAGCAGCACGTAAATATTGGC216890.20273509639054133No Hit
AACGGAATCCCAAAAGCAGCTG214580.20057585404344302No Hit
TGATGTAGTAGGTTGTATAGTT212930.19903353808122995No Hit
TGAGGTAGTAGGTTGTGTGGTT190160.17774957780268955No Hit
TTCACAGTGGCTAAGTTCTG187100.17488928274549442No Hit
GGAATACCGGGTGCTGTAGGCTTT182780.17085121913533655No Hit
TGAGGTAGTAGTTTGTGCTGTT180790.1689910926112129No Hit
CAAAGAATTCTCCTTTTGGGCT175570.16411176574893882No Hit
TAGCTTATCAGACTGATGTTGACA173800.16245728135311024No Hit
TTCTCACTACTGCACTTGACTAG169920.15883050199954254No Hit
TTCAAGTAATCCAGGATAGGC169550.1584846493292281No Hit
CTACGCCTGTCTGAGCGTCGCT168170.15719471234264987No Hit
CCCCCACTGCTAAATTTGACTGGCTT164080.15337163823025507No Hit
ACCGGGTGCTGTAGGCTT163500.1528294908011135No Hit
CCCATAAAGTAGAAAGCACTA162540.15193214333218952No Hit
TTCACAGTGGCTAAGTTCCGC146010.13648094160165494No Hit
GCCGGTCTGAGCGTCGCT145360.13587336258623767No Hit
TGTCAGTTTGTCAAATACCCCA145100.13563033098007077No Hit
TATTGCACTTGTCCCGGCCTGTT142140.13286350961755516No Hit
TGAGGTAGTAGGTTGTATAGT138760.12970409873738536No Hit
GGAATACCGGGTGCTGTAGGCTT137030.12808700381942864No Hit
TCCTGTCTGAGCGTCGCT133880.12514258243702187No Hit
GCCTGTCTGAGCGTCGCTT129830.12135689780249886No Hit
ACTGGACTTGGAGTCAGAAGGCT127220.11891723437136184No Hit
TTCACAGTGGCTAAGTTCTGC126770.11849660274530371No Hit
TACGCCTGTCTGAGCGTCGCT126570.11830965535594455No Hit
GCCTGTCTGAGCGTCGCG123000.11497264445588355No Hit
AGCTACATTGTCTGCTGGGTTT121890.11393508644494021No Hit
CACTAGATTGTGAGCTCCTGGA121560.1136266232524976No Hit
CCCCCCACTGCTAAATTTGACTGG121280.11336489690739478No Hit
ACCGGGTGCTGTAGGCTTT121170.11326207584324724No Hit
ACTGGACTTGGAGTCAGAAGGCA119590.11178519146730985No Hit
CTTCTCACTACTGCACTTGACTAG115100.10758822257619671No Hit
ATCCCACCGCTGCCACCA113460.1060552539834516No Hit
CTCCCACTGCTTCACTTGACTAG111620.10433533800134732No Hit
ACTGGACTTGGAGTCAGAAGG111360.10409230639518041No Hit
TTCAAGTAATCCAGGATAGGCTT108200.10113853764330569No Hit
TCGTTTCCCGGCCAATGCACCA107980.10093289551501063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)