FastQCFastQC Report
Thu 9 May 2024
EGAF00003947361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10664563
Total Bases240 Mbp
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5759905.4009714228327965No Hit
CAACGGAATCCCAAAAGCAGCTG5707285.351630441866207No Hit
TTCAAGTAATCCAGGATAGGCT3858123.6177009784648466No Hit
AAGCTGCCAGTTGAAGAACTGT3796013.559461367521576No Hit
TAGCAGCACGTAAATATTGGCG3681423.4520120515017823No Hit
AACATTCAACGCTGTCGGTGAGT3585253.3618348918750818No Hit
TAGCTTATCAGACTGATGTTGAC3505113.286688821661047No Hit
TAGCTTATCAGACTGATGTTGACT2962652.7780322550488004No Hit
TAGCTTATCAGACTGATGTTGA2389592.240682529607636No Hit
GCCTGTCTGAGCGTCGCT2004701.8797769772657351No Hit
AACATTCAACGCTGTCGGTGAG1999071.8744978111151858No Hit
TATTGCACTTGTCCCGGCCTGTAA1705101.5988465725224745No Hit
TGTAAACATCCTTGACTGGAAGCT1670021.5659525852114147No Hit
CCCCCACTGCTAAATTTGACTG1451781.3613122262956296No Hit
TATTGCACTTGTCCCGGCCTGTAT1444501.3544858800121486No Hit
TGAGGTAGTAGGTTGTATAGTT1027610.9635744099406605No Hit
CATAAAGTAGAAAGCACTACT1018830.9553415362636051No Hit
TATTGCACTTGTCCCGGCCTGT895060.8392842725951359No Hit
AGCAGCATTGTACAGGGCTATGA891250.8357116930154569No Hit
TTCAAGTAATTCAGGATAGGTT779430.7308597642491305No Hit
TGTAAACATCCCCGACTGGAAGCT738890.6928460172254597No Hit
TGAGGTAGTAGATTGTATAGTT737600.6916364036669856No Hit
TGTAAACATCCTACACTCTCAGCT714360.6698446059158729No Hit
TATTGCACTTGTCCCGGCCTGTA659160.618084397832335No Hit
AACCGGGCGGAAACACCA632500.5930857176238726No Hit
TAGCTTATCAGACTGATGTTGACA572360.5366933459908296No Hit
ACTGGACTTGGAGTCAGAAGGC495150.46429469261891No Hit
CAACGGAATCCCAAAAGCAGCTGT424760.39829105046310853No Hit
CCCATAAAGTAGAAAGCACTAC416570.39061141089419227No Hit
TTCTCACTACTGCACTTGACTAG409130.38363503502206325No Hit
TCCTGTCTGAGCGTCGCT387980.3638029987726642No Hit
CCCCCACTGCTAAATTTGACTGGCTT383090.3592177194696117No Hit
AACATTCAACGCTGTCGGTGAGTT368890.34590259347710733No Hit
CTTCTCACTACTGCACTTGACTAG364270.3415704891048981No Hit
CGCCTGTCTGAGCGTCGCT362610.34001393212267583No Hit
TCGTACGACTCTTAGCGG359950.3375196902114039No Hit
GGAATACCGGGTGCTGTAGGCTTT357300.3350348251494224No Hit
CCCATAAAGTAGAAAGCACT345270.32375447545295577No Hit
TGTCAGTTTGTCAAATACCCCAA339940.31875661478112133No Hit
CAAAGAATTCTCCTTTTGGGCT338520.3174251021818709No Hit
GTCAGGATGGCCGAGCGGTCTAAGG322410.3023189979748818No Hit
TTCACAGTGGCTAAGTTCCG308890.2896414977341312No Hit
TGAGGTAGTAGTTTGTACAGTT306710.28759734458880315No Hit
CATTGCACTTGTCTCGGTCTGA306110.28703473363137333No Hit
CTCCCACTGCTTCACTTGACTAG293910.2755949774969682No Hit
TGTAAACATCCTACACTCAGCT288230.27026892709996647No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC285890.26807474436599044No Hit
TGTCAGTTTGTCAAATACCCCA285570.2677746851886946No Hit
CCCCCCACTGCTAAATTTGACTGGCT284690.2669495224511309No Hit
TTCAAGTAATCCAGGATAGGCTT256080.24012235663102183No Hit
GGAATACCGGGTGCTGTAGGCTT236630.2218843847610071No Hit
CTACGCCTGTCTGAGCGTCGCT222250.20840047548127383No Hit
AGCAGCATTGTACAGGGCTATGT215530.20209923275806052No Hit
CACCCGGCTGTGTGCACATGTGC214800.20141472275985428No Hit
TAGCAGCACGTAAATATTGGC213470.20016760180421833No Hit
AGCTCGGTCTGAGGCCCCTCAGT212710.1994549612581406No Hit
CAAAGAATTCTCCTTTTGGGCTT208560.1955635688025848No Hit
TGTAAACATCCCCGACTGGAAGC204720.19196285867503432No Hit
TTCAAGTAATCCAGGATAGGC195840.18363621650507386No Hit
TTATAAAGCAATGAGACTGATT193750.1816764550033602No Hit
ATCACATTGCCAGGGATTTCCA181400.17009604612959764No Hit
TGAGGTAGTAGTTTGTGCTGTT179170.168005008737817No Hit
TACGCCTGTCTGAGCGTCGCT177930.16684227942579552No Hit
TGTCAGTTTGTCAAATACCCC177110.16607337778397482No Hit
TATTGCACTTGTCCCGGCCTGTT174300.16343848313334547No Hit
TATTGCACTTGTCCCGGCCTGTAG172430.1616850123160227No Hit
CAACGGAATCCCAAAAGCAGCT166600.15621830917966353No Hit
CAACACCAGTCGATGGGCTGT166190.15583385835875319No Hit
TAGCAGCACGTAAATATTGGCGT165880.15554317603074783No Hit
CACTAGATTGTGAGCTCCTGGAA164790.15452109945808376No Hit
CCCCCCACTGCTAAATTTGACTGG159060.14914816481462953No Hit
TGTAAACATCCTTGACTGGAAGC147810.13859920936282152No Hit
TGATGTAGTAGGTTGTATAGTT143570.1346234252636512No Hit
CAAAGTGCTGTTCGTGCAGGTAG142070.13321689787007682No Hit
TCGTTTCCCGGCCAATGCACCA140780.13200728431160283No Hit
TATTGCACTTGTCCCGGCCTGTAAA137340.1287816481556722No Hit
TTCACAGTGGCTAAGTTCTG135550.12710319213267343No Hit
ATCACATTGCCAGGGATTTCC132970.12468396501572544No Hit
TAGCTTATCAGACTGATGTTG132830.1245526891256585No Hit
TATTGCACTTGTCCCGGCCTGTAAT132820.124543312276368No Hit
TATTGCACTTGTCCCGGCCTGTTT131420.12323055337569856No Hit
GCGTTCGCGCTTTCCCCTG131150.12297737844485516No Hit
AACGGAATCCCAAAAGCAGCTG128710.12068942721797414No Hit
AGCAGCATTGTACAGGGCTATG127530.1195829590016956No Hit
TTCACAGTGGCTAAGTTCTGC127410.11947043681020965No Hit
TGTCAGTTTGTCAAATACC126540.11865465092193651No Hit
CACTAGATTGTGAGCTCCTGGA126410.11853275188116005No Hit
ACTGGACTTGGAGTCAGAAGGCA118560.1111719251881207No Hit
ACCCCCCACTGCTAAATTTGACTG117820.110478038340624No Hit
ACGCCTGTCTGAGCGTCGCT117080.10978415149312727No Hit
GTCAGGATGGCCGAGTGGTCTAAGG116930.10964349875376984No Hit
TTCAAGTAATCCAGGATAGGCTA113170.10611780342054335No Hit
AACATTCAACGCTGTCGGTGAGA113120.10607091917409087No Hit
TCGTTTCCCGGGCGGCGCACCA111570.10461750753406399No Hit
ACCACAGGGTAGAACCACGGAC109510.10268587658022181No Hit
TGAGGTAGTAGGTTGTGTGGTT108860.10207638137633956No Hit
CCCATAAAGTAGAAAGCACTA108420.10166380000755774No Hit
TTCACAGTGGCTAAGTTCCGC108360.10160753891181476No Hit
TGAGGTAGTAGGTTGTATAGT107670.10096053631077054No Hit
AACATTCATTGCTGTCGGTGGGTT106950.10028540316185483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)