FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005674
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6701615.582035627487469No Hit
CCCCCCACTGCTAAATTTGACTG5986104.986059091726129No Hit
GCCTGTCTGAGCGTCGCT5885874.902573566465323No Hit
AACATTCAACGCTGTCGGTGAGT4946584.120201831234131No Hit
AAGCTGCCAGTTGAAGAACTGT4174423.4770392732636255No Hit
TTCAAGTAATCCAGGATAGGCT3618963.0143747031611885No Hit
TAGCAGCACGTAAATATTGGCG3605433.003105031837446No Hit
GGAATACCGGGTGCTGTAGGCTTT2864002.3855387044492464No Hit
AACATTCAACGCTGTCGGTGAG2777422.3134228032511963No Hit
TCCTGTCTGAGCGTCGCT2749022.2897673216847303No Hit
TAGCTTATCAGACTGATGTTGAC2298311.9143531633459312No Hit
TAGCTTATCAGACTGATGTTGACT2100721.7497726491657195No Hit
GGAATACCGGGTGCTGTAGGCTT1985431.6537430551587524No Hit
CCCCCACTGCTAAATTTGACTG1902981.5850671940617411No Hit
TAGCTTATCAGACTGATGTTGA1782401.484631350143274No Hit
TGAATACCGGGTGCTGTAGGCTTT1685341.4037862430714012No Hit
TGAATACCGGGTGCTGTAGGCTT1175560.9791703489533365No Hit
CATAAAGTAGAAAGCACTACT1134770.945194747083754No Hit
TATTGCACTTGTCCCGGCCTGTAT1020230.8497898576956197No Hit
TATTGCACTTGTCCCGGCCTGTAA1006150.8380620696513997No Hit
TGTAAACATCCTTGACTGGAAGCT992370.8265841634547131No Hit
TATTGCACTTGTCCCGGCCTGT836030.6963624033103014No Hit
AGCAGCATTGTACAGGGCTATGA735160.6123437967747584No Hit
TGAGGTAGTAGGTTGTATAGTT731550.6093368852094435No Hit
CGCCTGTCTGAGCGTCGCT694220.5782432539814092No Hit
CCCATAAAGTAGAAAGCACTAC588960.49056804307696505No Hit
ACTGGACTTGGAGTCAGAAGGC564540.4702276606877715No Hit
TTCAAGTAATTCAGGATAGGTT518780.43211234954405725No Hit
TATTGCACTTGTCCCGGCCTGTA514010.4281392281682811No Hit
TGTAAACATCCCCGACTGGAAGCT485130.4040839356457621No Hit
CAACGGAATCCCAAAAGCAGCTGT447920.37309025715674105No Hit
TGAGGTAGTAGATTGTATAGTT443040.36902551243686943No Hit
CCCATAAAGTAGAAAGCACT431080.35906355611521684No Hit
TGTAAACATCCTACACTCTCAGCT382750.31880759047763585No Hit
GGAATACCGGGTGCTGTAGGCTTA382730.3187909316878003No Hit
AACATTCAACGCTGTCGGTGAGTT361700.3012742141757306No Hit
AACCGGGCGGAAACACCA338980.2823498289225578No Hit
CATTGCACTTGTCTCGGTCTGA332990.2773605213668137No Hit
CAACACCAGTCGATGGGCTGT295200.24588373797256197No Hit
TCGTACGACTCTTAGCGG294830.2455755503606045No Hit
CCCTGTCTGAGCGTCGCT290650.24209386328497676No Hit
TGTCAGTTTGTCAAATACCCCAA290300.2418023344628548No Hit
GGAATACCGGGTGCTGTAGGCT287470.239445115701126No Hit
TACGCCTGTCTGAGCGTCGCT253470.21112517298070896No Hit
CTACGCCTGTCTGAGCGTCGCT249400.20773510924917668No Hit
TTAATACCGGGTGCTGTAGGCTTT248150.20669393488445548No Hit
TAGCTTATCAGACTGATGTTGACA248030.20659398214544225No Hit
TTCAAGTAATCCAGGATAGGCTT233420.19442473617058068No Hit
TGAATACCGGGTGCTGTAGGCTTA228930.1906848378525021No Hit
TTCTCACTACTGCACTTGACTAG226360.18854418335863526No Hit
TGAGGTAGTAGTTTGTACAGTT217560.1812143158309979No Hit
CTTCTCACTACTGCACTTGACTAG216680.18048132907823417No Hit
GTAATACCGGGTGCTGTAGGCTTT202400.16858695313565902No Hit
TAGCAGCACGTAAATATTGGC201590.16791227214731969No Hit
AACATTCAACGCTGTCGGTGA200250.16679613322833853No Hit
CAAAGAATTCTCCTTTTGGGCT196330.1635310104205728No Hit
CAACGGAATCCCAAAAGCAGCT193200.16092390981131088No Hit
ACGCCTGTCTGAGCGTCGCT187060.1558096613318003No Hit
TTCACAGTGGCTAAGTTCCG183760.15306096100893626No Hit
CTCCCACTGCTTCACTTGACTAG182260.15181155177127081No Hit
CCCCCACTGCTAAATTTGACTGGCTT179770.14973753243674615No Hit
TGTAAACATCCTACACTCAGCT178980.14907951023824237No Hit
ACCGGGTGCTGTAGGCTT175990.1465890211578292No Hit
TGTCAGTTTGTCAAATACCCCA175560.1462308571763651No Hit
TGAATACCGGGTGCTGTAGGCT173170.14424013179101816No Hit
TTCAAGTAATCCAGGATAGGC173010.14410686147233384No Hit
AGCAGCATTGTACAGGGCTATGT172740.14388196780955403No Hit
TTATAAAGCAATGAGACTGATT172020.14328225137547462No Hit
TTAATACCGGGTGCTGTAGGCTT169580.14124987901553882No Hit
AACGGAATCCCAAAAGCAGCTG166420.1386177902215236No Hit
AACATTCAACGCTGTCGGTGAGA164740.13721845187533827No Hit
CAAAGAATTCTCCTTTTGGGCTT164350.13689360547354526No Hit
CCCCCCACTGCTAAATTTGACTGGCT161000.1341032581760924No Hit
ACCACAGGGTAGAACCACGGAC157210.1309464175022577No Hit
GCGTTCGCGCTTTCCCCTG156090.13001352527146748No Hit
TGATGTAGTAGGTTGTATAGTT155950.1298969137426187No Hit
TAGCAGCACGTAAATATTGGCGT153000.12743974224187662No Hit
ACCGGGTGCTGTAGGCTTT151910.12653183819583974No Hit
TATTGCACTTGTCCCGGCCTGTT150070.1249992295309701No Hit
TGTAAACATCCCCGACTGGAAGC146250.12181740067238207No Hit
ACTGGACTTGGAGTCAGAAGGCA144470.12033476837701906No Hit
CCCCCCACTGCTAAATTTGACTGG144070.12000159258030829No Hit
TTCACAGTGGCTAAGTTCCGC139410.1161200945486276No Hit
CACTAGATTGTGAGCTCCTGGA138940.11572861298749242No Hit
GTAATACCGGGTGCTGTAGGCTT138010.11495397926013984No Hit
TGCAAGTAATCCAGGATAGGCT135170.11258843110349323No Hit
CAAAGTGCTGTTCGTGCAGGT133890.1115222685540187No Hit
ACCGGGTGCTGTAGGCTTA133830.11147229218451209No Hit
ACTGGACTTGGAGTCAGAAGG130570.10875690944131917No Hit
TGAGGTAGTAGTTTGTGCTGTT130530.10872359186164808No Hit
ACTGGACTTGGAGTCAGAAGGCT129920.10821549877166411No Hit
ACTTGACTTGGAGTCAGAAGGC128660.10716599501202516No Hit
TTTAAACATCCTTGACTGGAAGCT124660.10383423704491727No Hit
ACCTGTCTGAGCGTCGCT122750.10224332261562326No Hit
AGCTCGGTCTGAGGCCCCTCAGT121920.10155198283744837No Hit
AGCAGCATTGTACAGGGCTATG121520.10121880704073757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)