FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5550832
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5382069.695951886131665No Hit
CAACGGAATCCCAAAAGCAGCTG4708448.482404079244336No Hit
AACATTCAACGCTGTCGGTGAGT1883763.393653419883722No Hit
CCCCCACTGCTAAATTTGACTG1523332.7443273368749046No Hit
AACATTCAACGCTGTCGGTGAG1505912.71294465406267No Hit
TAGCTTATCAGACTGATGTTGACT1469502.6473508836152853No Hit
TTCAAGTAATCCAGGATAGGCT1302502.3464950839802032No Hit
TATTGCACTTGTCCCGGCCTGTAA1095791.9741004591744085No Hit
TAGCTTATCAGACTGATGTTGAC1078931.9437266341334056No Hit
TAGCAGCACGTAAATATTGGCG1023791.8443901743017983No Hit
TATTGCACTTGTCCCGGCCTGTAT1015831.8300499816964375No Hit
AAGCTGCCAGTTGAAGAACTGT984661.7738962375370033No Hit
TAGCTTATCAGACTGATGTTGA798381.4383069060638116No Hit
GGAATACCGGGTGCTGTAGGCTTT798221.438018660986317No Hit
TGAGGTAGTAGGTTGTATAGTT453950.8178053308044633No Hit
TGAATACCGGGTGCTGTAGGCTTT450150.8109595102139643No Hit
GGAATACCGGGTGCTGTAGGCTT428250.7715059652318788No Hit
TGTAAACATCCTTGACTGGAAGCT396620.7145235164746474No Hit
GCCTGTCTGAGCGTCGCT393010.7080199869136734No Hit
TATTGCACTTGTCCCGGCCTGTA363310.6545144944037218No Hit
AGCAGCATTGTACAGGGCTATGA361680.6515779976767446No Hit
TATTGCACTTGTCCCGGCCTGT357730.6444619473260945No Hit
TGAGGTAGTAGATTGTATAGTT308420.5556284175057001No Hit
CCCCCACTGCTAAATTTGACTGGCTT295440.5322445355939434No Hit
ACTGGACTTGGAGTCAGAAGGC284960.5133644830180413No Hit
TGTAAACATCCCCGACTGGAAGCT262460.47283001899535065No Hit
TTCAAGTAATTCAGGATAGGTT249600.449662320891715No Hit
CAACGGAATCCCAAAAGCAGCTGT247750.4463294871831826No Hit
CATAAAGTAGAAAGCACTACT244120.4397899269875219No Hit
TTAATGCTAATCGTGATAGGGGTT240500.43326838210920454No Hit
TGAATACCGGGTGCTGTAGGCTT238500.42966531864052093No Hit
TCCTGTCTGAGCGTCGCT201370.3627744453444096No Hit
TAGCTTATCAGACTGATGTTGACA199520.3594416116358773No Hit
ACCCCCCACTGCTAAATTTGACTG182340.32849129643988506No Hit
CCCATAAAGTAGAAAGCACTAC170110.30645856332888477No Hit
CAAAGAATTCTCCTTTTGGGCT168310.3032158062070695No Hit
CATTGCACTTGTCTCGGTCTGA145160.2615103465570567No Hit
TGAGAACTGAATTCCATGGGTT141160.2543042196196894No Hit
TGAGAACTGAATTCCATAGGCTGT136420.24576495919890928No Hit
CCCATAAAGTAGAAAGCACT129360.2330461451544561No Hit
TATTGCACTTGTCCCGGCCTGTAG123960.22331787378901038No Hit
TATTGCACTTGTCCCGGCCTGTAAT122120.22000305539782145No Hit
CCCCCCACTGCTAAATTTGACTGGCT118140.21283295909514105No Hit
TGTAAACATCCTACACTCTCAGCT116840.2104909678404967No Hit
TAGCAGCACGTAAATATTGGC115330.20777065492164057No Hit
CTCCCACTGCTTCACTTGACTAGCCT113160.20386133105811888No Hit
AACATTCAACGCTGTCGGTGAGTT113100.20375323915405832No Hit
GGAATACCGGGTGCTGTAGGCTTA108560.19557428508014657No Hit
CTCCCACTGCTTCACTTGACTAG107170.19307015596941143No Hit
TGAGGTAGTAGTTTGTACAGTT104820.1888365563937082No Hit
CACTAGATTGTGAGCTCCTGGAA102400.18447684959660102No Hit
TTCTCACTACTGCACTTGACTAG102200.18411654324973264No Hit
CAACACCAGTCGATGGGCTGT100970.18190065921649223No Hit
TATTGCACTTGTCCCGGCCTGTAAA100460.1809818780319779No Hit
TGATGTAGTAGGTTGTATAGTT97770.17613575766659845No Hit
CAAAGAATTCTCCTTTTGGGCTT96200.17330735284368182No Hit
ACTGGACTTGGAGTCAGAAGGCA95360.17179406618683468No Hit
AACGGAATCCCAAAAGCAGCTG93910.16918184517203907No Hit
CACTAGATTGTGAGCTCCTGGA93850.16907375326797855No Hit
AACCGGGCGGAAACACCA91880.16552473575132523No Hit
ACTGGACTTGGAGTCAGAAGGCT91370.16460595456681087No Hit
CTTCTCACTACTGCACTTGACTAG91250.16438977075868985No Hit
TTCAAGTAATCCAGGATAGGCTT87930.15840868540067507No Hit
AGCTCGGTCTGAGGCCCCTCAGT87310.15729173572538313No Hit
TAGCAGCACATCATGGTTTACA81330.14651857595401915No Hit
TATTGCACTTGTCCCGGCCTGTT80740.14545567223075748No Hit
CACCCGGCTGTGTGCACATGTGC78700.1417805474927002No Hit
CGCCCCACTGCTAAATTTGACTG74050.13340342492801077No Hit
TATTGCACTTGTCCCGGCCTGTTT73520.13244861310880962No Hit
CCCCCCACTGCTAAATTTGACTGG73130.13174601573241634No Hit
AGCAGCATTGTACAGGGCTATGT72880.13129563279883086No Hit
CAACGGAATCCCAAAAGCAGCT71640.12906173344824703No Hit
GACCCGCGGGCGCTCTCCAGTCCT70870.12767455401280386No Hit
ACCGGGTGCTGTAGGCTTA67890.12230598944446526No Hit
TTCACAGTGGCTAAGTTCCGC67840.12221591285774817No Hit
TGAGGTAGTAGTTTGTGCTGTT67610.12180156055884957No Hit
TGATGTAGTAGATTGTATAGTT66670.12010812072856826No Hit
ACTTGACTTGGAGTCAGAAGGC65390.11780216010861075No Hit
TGAATACCGGGTGCTGTAGGCTTA64370.1159645977395821No Hit
TCAGTGCACTACAGAACTTTGTC63890.11509986250709803No Hit
ACCGGGTGCTGTAGGCTT63530.11445131108273499No Hit
TCGTACGACTCTTAGCGG63450.11430718854398764No Hit
ACCGGGTGCTGTAGGCTTT62750.11304611632994836No Hit
GGAATACCGGGTGCTGTAGGCT61070.11001954301625415No Hit
ATGCACCTGGGCAAGGATTCTGA60750.10944305286126475No Hit
TTAATACCGGGTGCTGTAGGCTTT59890.10789373556973081No Hit
ATCACATTGCCAGGGATTTCC59660.10747938327083219No Hit
TTATAAAGCAATGAGACTGATT58860.10603815788335874No Hit
AACATTCAACGCTGTCGGTGAGA58280.1049932694774405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)