FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7462917
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5062326.783299345282816No Hit
CCCCCCACTGCTAAATTTGACTG4383715.873990022936072No Hit
AACATTCAACGCTGTCGGTGAGT2506253.3582713032986966No Hit
AACATTCAACGCTGTCGGTGAG2222042.977441662556344No Hit
TTCAAGTAATCCAGGATAGGCT2217482.9713314512274493No Hit
TAGCAGCACGTAAATATTGGCG1966902.6355646190356934No Hit
AAGCTGCCAGTTGAAGAACTGT1955972.620918871267093No Hit
GCCTGTCTGAGCGTCGCT1799352.41105455145756No Hit
CCCCCACTGCTAAATTTGACTG1549972.0768956696155136No Hit
TATTGCACTTGTCCCGGCCTGTAT1419141.9015888827384788No Hit
TAGCTTATCAGACTGATGTTGACT1266651.697258592049195No Hit
TAGCTTATCAGACTGATGTTGA1141381.529401975125812No Hit
TAGCTTATCAGACTGATGTTGAC1133801.5192450887501496No Hit
TATTGCACTTGTCCCGGCCTGTAA1122861.5045859413953016No Hit
TATTGCACTTGTCCCGGCCTGT916691.2283266717290302No Hit
TGAGGTAGTAGGTTGTATAGTT732660.981734086014892No Hit
TTCAAGTAATTCAGGATAGGTT656960.8802992181207429No Hit
TATTGCACTTGTCCCGGCCTGTA599400.8031711996796963No Hit
CATAAAGTAGAAAGCACTACT566190.7586711737514968No Hit
TGAGGTAGTAGATTGTATAGTT562330.7534989334599327No Hit
TCCTGTCTGAGCGTCGCT557240.7466785440599165No Hit
TGTAAACATCCTTGACTGGAAGCT542380.7267667588960188No Hit
TTAATGCTAATCGTGATAGGGGTT489930.6564859290274835No Hit
AGCAGCATTGTACAGGGCTATGA483670.648097788036501No Hit
ACCGGGTGCTGTAGGCTTA434800.5826140100445978No Hit
GGAATACCGGGTGCTGTAGGCTTT399620.5354742656256261No Hit
TGAGAACTGAATTCCATGGGTT379790.5089028860966831No Hit
CCCATAAAGTAGAAAGCACT345220.4625805164388134No Hit
ACCGGGTGCTGTAGGCTT338970.45420577503407844No Hit
ACCGGGTGCTGTAGGCTTT302500.40533748398916936No Hit
TGTAAACATCCCCGACTGGAAGCT298810.40039303666381393No Hit
CCCATAAAGTAGAAAGCACTAC294090.3940684319549581No Hit
CAACGGAATCCCAAAAGCAGCTGT289640.38810561607478683No Hit
TCGTACGACTCTTAGCGG289100.38738203841741775No Hit
CATTGCACTTGTCTCGGTCTGA288280.38628327234511656No Hit
ACTGGACTTGGAGTCAGAAGGC256350.3434983934566069No Hit
ATCCCGGACGAGCCCCCA249420.3342124801870368No Hit
GGAATACCGGGTGCTGTAGGCTT239550.3209870885606794No Hit
TGAGGTAGTAGTTTGTACAGTT217430.2913472037810417No Hit
TGAGAACTGAATTCCATAGGCTGT208230.2790195844332719No Hit
CAACACCAGTCGATGGGCTGT204760.27436992800536303No Hit
TGATGTAGTAGGTTGTATAGTT198820.266410573774303No Hit
AACCGGGCGGAAACACCA189460.253868561046572No Hit
TGTAAACATCCTACACTCTCAGCT186850.25037126903595475No Hit
CTCCCACTGCTTCACTTGACTAG180800.24226451935617133No Hit
TTCTCACTACTGCACTTGACTAG180540.24191613011373433No Hit
AGCTCGGTCTGAGGCCCCTCAGT177970.23847243644810737No Hit
TATTGCACTTGTCCCGGCCTGTT174830.2342649663663685No Hit
CGCCTGTCTGAGCGTCGCT174660.23403717340015973No Hit
TATTGCACTTGTCCCGGCCTGTAG161280.21610852700090327No Hit
CACTAGATTGTGAGCTCCTGGA160200.21466137168616506No Hit
TGAATACCGGGTGCTGTAGGCTTT157410.2109228871230914No Hit
TGATGTAGTAGATTGTATAGTT149690.20057840653996284No Hit
TAGCTTATCAGACTGATGTTGACA147490.19763049756549617No Hit
CAACGGAATCCCAAAAGCAGCT147350.1974429033580301No Hit
TCAGTGCACTACAGAACTTTGT146710.19658532983818527No Hit
CACTAGATTGTGAGCTCCTGGAA146560.1963843360444716No Hit
CCCCCACTGCTAAATTTGACTGGCTT133870.17938026109629787No Hit
TTCAAGTAATCCAGGATAGGCTT131660.1764189525355836No Hit
TATTGCACTTGTCCCGGCCTGTTT131500.1762045591556224No Hit
TAGCAGCACGTAAATATTGGC130870.17536038522202513No Hit
AACATTCAACGCTGTCGGTGAGTT129740.17384623197604906No Hit
AACGGAATCCCAAAAGCAGCTG126600.1696387618943102No Hit
TTCAAGTAATCCAGGATAGGC126010.16884818630570325No Hit
CAAAGAATTCTCCTTTTGGGCT125180.16773602064715445No Hit
TGAGGTAGTAGTTTGTGCTGTT111240.14905699741803372No Hit
AGCAGCATTGTACAGGGCTATGT110860.14854781314062585No Hit
TGAGAACTGAATTCCATGGGTTG110650.14826642182942676No Hit
TGAGGTAGTAGGTTGTATAGT108380.14522471575122703No Hit
TTATAAAGCAATGAGACTGATT107680.14428674471389671No Hit
CACCCGGCTGTGTGCACATGTGC107570.14413934926517338No Hit
ACCCCCCACTGCTAAATTTGACTG104980.14066885642705124No Hit
CTTCTCACTACTGCACTTGACTAG103550.1387527155936479No Hit
AACATTCAACGCTGTCGGTGA99920.13388866578577788No Hit
GACCCGCGGGCGCTCTCCAGTCCT98830.1324281108847921No Hit
ACCTGGTGCTGTAGGCTTA97490.13063256632761694No Hit
TGATAACTGAATTCCATGGGTT95870.12846183335550965No Hit
TGAGGTAGTAGGTTGTGTGGTT94360.12643849583212569No Hit
TGAATACCGGGTGCTGTAGGCTT93940.1258757132097275No Hit
TATTGCACTTGTCCCGGCCTGA93870.12578191610599448No Hit
TATTGCACTTGTCCCGGCCTGTAAT90310.12101166340185748No Hit
TTCACAGTGGCTAAGTTCCG88750.11892132794723564No Hit
TGTAAACATCCCCGACTGGAAGC87540.11729997801127898No Hit
CGCGACCTCAGATCAGACGTGG84170.11278431744584591No Hit
CCCATAAAGTAGAAAGCACTA81910.10975601095389376No Hit
CAAAGTGCTGTTCGTGCAGGT81190.1087912407440683No Hit
CCCCCCACTGCTAAATTTGACTGGCT80740.10818825936292739No Hit
AACATTCAACGCTGTCGGTGAGA80440.10778627177550013No Hit
CAAAGAATTCTCCTTTTGGGCTT80270.10755847880929133No Hit
CCCCCCACTGCTAAATTTGACTGG79690.10678130280693193No Hit
AGCTACATCTGGCTACTGGGTCTC79620.1066875057031989No Hit
TCAGTGCACTACAGAACTTTGTC78730.10549494252716464No Hit
TTCACAGTGGCTAAGTTCTG77750.10418178307490221No Hit
TGTCAGTTTGTCAAATACCCCA77050.10324381203757191No Hit
TTAATGCTAATCGTGATAGGGGTTT76720.1028016256914019No Hit
TATTGCACTTGTCCCGGCCTGTAAA75450.10109987823795977No Hit
CAAAGTGCTGTTCGTGCAGGTAG75360.10097928196173159No Hit
ACCTGGTGCTGTAGGCTT75100.10063089271929461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)