FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350914
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4746206.456612062119079No Hit
CCCCCCACTGCTAAATTTGACTG4101735.579891153671503No Hit
AACATTCAACGCTGTCGGTGAGT2432333.3088810452686563No Hit
AACATTCAACGCTGTCGGTGAG2229253.0326160801228257No Hit
TTCAAGTAATCCAGGATAGGCT1792932.439057238324377No Hit
TAGCAGCACGTAAATATTGGCG1553882.1138595826314934No Hit
AAGCTGCCAGTTGAAGAACTGT1454711.9789511889269824No Hit
CCCCCACTGCTAAATTTGACTG1376361.872365803762634No Hit
TATTGCACTTGTCCCGGCCTGTAT1014321.3798556206751977No Hit
TATTGCACTTGTCCCGGCCTGT910531.2386622942398728No Hit
GGAATACCGGGTGCTGTAGGCTTT906401.233043945283539No Hit
GCCTGTCTGAGCGTCGCT855041.163175082717605No Hit
ACCGGGTGCTGTAGGCTTA811611.104093994297852No Hit
ACCGGGTGCTGTAGGCTT792751.0784373208556106No Hit
TAGCTTATCAGACTGATGTTGA781051.0625209327710814No Hit
TATTGCACTTGTCCCGGCCTGTAA778451.0589839576411857No Hit
TAGCTTATCAGACTGATGTTGACT715310.9730898769867257No Hit
GGAATACCGGGTGCTGTAGGCTT689040.9373528244242825No Hit
ACCGGGTGCTGTAGGCTTT680430.9256399952441288No Hit
TAGCTTATCAGACTGATGTTGAC675180.9184980262318401No Hit
TTAATGCTAATCGTGATAGGGGTT654860.8908552052166573No Hit
TCGTACGACTCTTAGCGG623050.847581674877437No Hit
TATTGCACTTGTCCCGGCCTGTA604450.8222786989481852No Hit
ATCCCGGACGAGCCCCCA581090.7905003377811248No Hit
TGAGGTAGTAGGTTGTATAGTT517030.7033547120807018No Hit
TGAATACCGGGTGCTGTAGGCTTT507100.6898461878346012No Hit
TTCAAGTAATTCAGGATAGGTT477760.6499327838687815No Hit
TCCTGTCTGAGCGTCGCT420990.5727042922825651No Hit
CATAAAGTAGAAAGCACTACT389730.5301789682208226No Hit
TGAATACCGGGTGCTGTAGGCTT385570.5245198080129899No Hit
AGCAGCATTGTACAGGGCTATGA347880.473247272380006No Hit
TGAGGTAGTAGATTGTATAGTT338290.46020127565089186No Hit
CGCCTGTCTGAGCGTCGCT334490.4550318504610447No Hit
TGTAAACATCCTTGACTGGAAGCT299460.40737791246095384No Hit
TGAGAACTGAATTCCATGGGTT269880.3671380184831437No Hit
CCCATAAAGTAGAAAGCACT245470.3339312635136257No Hit
CAACGGAATCCCAAAAGCAGCTGT243700.33152339967519684No Hit
CATTGCACTTGTCTCGGTCTGA237810.3235107906309338No Hit
ACCTGGTGCTGTAGGCTTA232330.3160559353571542No Hit
ACCTGGTGCTGTAGGCTT226420.308016118811892No Hit
ACTGGACTTGGAGTCAGAAGGC220940.3005612635381124No Hit
CAACACCAGTCGATGGGCTGT214640.2919909007233658No Hit
AACCGGGCGGAAACACCA210800.2867670605315203No Hit
TGTAAACATCCCCGACTGGAAGCT200340.2725375375089411No Hit
ACCTGGTGCTGTAGGCTTT194550.264660965969674No Hit
TATTGCACTTGTCCCGGCCTGTT176350.2399021400604061No Hit
CCCATAAAGTAGAAAGCACTAC167860.22835255588624762No Hit
TGATGTAGTAGGTTGTATAGTT166520.22652965331930153No Hit
AGCTCGGTCTGAGGCCCCTCAGT158150.2151433141511382No Hit
CAACGGAATCCCAAAAGCAGCT157650.21446312662615832No Hit
CTCCCACTGCTTCACTTGACTAG151850.20657295133639167No Hit
CACTAGATTGTGAGCTCCTGGA142730.1941663308807585No Hit
GGAATACCGGGTGCTGTAGGCTTA139450.18970430071689046No Hit
AACATTCAACGCTGTCGGTGAGTT138440.18833032191643106No Hit
TGAGAACTGAATTCCATAGGCTGT135180.18389549925356222No Hit
ATACCGGGTGCTGTAGGCTT131390.17873967781421468No Hit
TGAGGTAGTAGTTTGTACAGTT131090.17833156529922675No Hit
TATTGCACTTGTCCCGGCCTGTAG128360.17461774141283656No Hit
GGAATACCGGGTGCTGTAGGCT123630.1681831674265268No Hit
TTCTCACTACTGCACTTGACTAG120330.16369392976165956No Hit
CGCGACCTCAGATCAGACGTGG116960.15910946584329513No Hit
AACGGAATCCCAAAAGCAGCTG116510.15849729707081323No Hit
CACTAGATTGTGAGCTCCTGGAA114860.1562526782383796No Hit
TTCAAGTAATCCAGGATAGGC112780.15342309813446328No Hit
TGATGTAGTAGATTGTATAGTT112390.15289255186497896No Hit
TGTAAACATCCTACACTCTCAGCT108120.14708375040165073No Hit
CCCCCACTGCTAAATTTGACTGGCTT107270.14592743160918492No Hit
ATACCGGGTGCTGTAGGCTTA106410.1447575090662195No Hit
ATACCGGGTGCTGTAGGCTTT106240.14452624530772634No Hit
TTCACAGTGGCTAAGTTCCG106180.14444462280472878No Hit
TAGCAGCACGTAAATATTGGC105450.14345154901825813No Hit
TTAATGCTAATCGTGATAGGGGT101010.13741148379643675No Hit
ACCCCCCACTGCTAAATTTGACTG99870.1358606562394826No Hit
TGAGGTAGTAGGTTGTGTGGTT98970.1346363186945188No Hit
AGCTACATCTGGCTACTGGGTCTC98700.13426901743102965No Hit
CTACGCCTGTCTGAGCGTCGCT96730.13158907858260893No Hit
ATCCCACCGCTGCCACCA93640.12738551967823322No Hit
TATTGCACTTGTCCCGGCCTGA93330.12696380341274568No Hit
TCGTTTCCCGGGCGGCGCACCA92420.1257258621172823No Hit
CACCCGGCTGTGTGCACATGTGC92290.12554901336078753No Hit
GACCCGCGGGCGCTCTCCAGTCCT91670.12470558082981245No Hit
AACATTCAACGCTGTCGGTGA91310.12421584581182694No Hit
TATTGCACTTGTCCCGGCCTGTTT89080.12118220945041665No Hit
CAAAGAATTCTCCTTTTGGGCT88710.12067887068193152No Hit
CAAAGTGCTGTTCGTGCAGGT86400.11753640431652446No Hit
TAGCTTATCAGACTGATGTTGACA84770.11531899298509003No Hit
TGATAACTGAATTCCATGGGTT84760.11530538923459042No Hit
TTCACAGTGGCTAAGTTCTG84740.11527818173359121No Hit
TTCAAGTAATCCAGGATAGGCTT84590.11507412547609726No Hit
TGAGGTAGTAGGTTGTATAGT83060.11299275164965882No Hit
TATTGCACTTGTCCCGGCCTG81940.11146913159370386No Hit
GAATACCGGGTGCTGTAGGCTTT81720.11116984908271271No Hit
TTCACCACCTTCTCCACCCAGC81400.11073452906672557No Hit
AGCAGCATTGTACAGGGCTATGT80970.11014956779524289No Hit
TGAGGTAGTAGTTTGTGCTGTT79870.10865315524028712No Hit
ACCGGGTGCTGTAGGCTA79820.10858513648778914No Hit
TGAATACCGGGTGCTGTAGGCTTA79190.10772810020631449No Hit
TGAGAACTGAATTCCATGGGTTG79160.10768728895481569No Hit
CCACCCTGAACGCGCCCGA78800.10719755393683017No Hit
CCCCCCACTGCTAAATTTGACTGG78470.10674863017034344No Hit
ACTTGACTTGGAGTCAGAAGGC78180.10635412140585511No Hit
AGCTACATTGTCTGCTGGGTTT76780.10444959633591142No Hit
AACATTCAACGCTGTCGGTGAGA75020.10205533624798221No Hit
TTAATGCTAATCGTGATAGGGGTTT74660.10156560122999671No Hit
CCCCCCACTGCTAAATTTGACTGGCT74280.101048658711012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)