FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6884088
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT140530220.413771584558475No Hit
CAACGGAATCCCAAAAGCAGCTG3591505.217103558234584No Hit
CCCCCCACTGCTAAATTTGACTG2254123.27439161149596No Hit
TTCAAGTAATCCAGGATAGGCT2153603.1283737221255743No Hit
TAGCAGCACGTAAATATTGGCG1788832.5984996124395856No Hit
AACATTCAACGCTGTCGGTGAGT1540262.2374205559254907No Hit
AAGCTGCCAGTTGAAGAACTGT1528352.2201197892879927No Hit
AACATTCAACGCTGTCGGTGAG1430732.0783145131206924No Hit
TAGCTTATCAGACTGATGTTGA1217801.7690070202472719No Hit
CCCCCACTGCTAAATTTGACTG956991.390147830765673No Hit
CATAAAGTAGAAAGCACTACT935531.358974493062843No Hit
TAGCTTATCAGACTGATGTTGACT849121.23345314586333No Hit
TAGCTTATCAGACTGATGTTGAC841741.2227327715740997No Hit
TATTGCACTTGTCCCGGCCTGT821481.1933025841622014No Hit
TATTGCACTTGTCCCGGCCTGTAT804441.16854985003097No Hit
TGAGGTAGTAGGTTGTATAGTT703741.0222704881169444No Hit
TATTGCACTTGTCCCGGCCTGTAA614570.8927398952482886No Hit
TGAGGTAGTAGATTGTATAGTT595730.8653724356806595No Hit
ATCCCGGACGAGCCCCCA583090.847011252616178No Hit
TTCAAGTAATTCAGGATAGGTT534140.7759052469985858No Hit
TATTGCACTTGTCCCGGCCTGTA489490.7110455299234989No Hit
TCGTACGACTCTTAGCGG458910.6666242500095872No Hit
ACCGGGTGCTGTAGGCTT401870.5837665061806299No Hit
TGTAAACATCCTTGACTGGAAGCT392490.5701408814065131No Hit
CCCATAAAGTAGAAAGCACT384340.5583019856806014No Hit
ACCGGGTGCTGTAGGCTTA378450.5497460230026113No Hit
AGCAGCATTGTACAGGGCTATGA315230.4579110551753551No Hit
ACCGGGTGCTGTAGGCTTT311160.4519988704386114No Hit
CATTGCACTTGTCTCGGTCTGA302200.4389833482663208No Hit
CAACACCAGTCGATGGGCTGT274270.3984115252448836No Hit
CGCCTGTCTGAGCGTCGCT238030.3457683864587437No Hit
AACCGGGCGGAAACACCA234840.34113451193535005No Hit
ACTGGACTTGGAGTCAGAAGGC227170.32999287632581104No Hit
TCAGTGCACTACAGAACTTTGT224600.3262596294527321No Hit
TGAGGTAGTAGTTTGTACAGTT223520.3246907941908936No Hit
TTCACAGTGGCTAAGTTCCG199880.2903507334595374No Hit
TGTAAACATCCCCGACTGGAAGCT195230.28359602608217677No Hit
AGAATTACTCTGAGACCT194770.2829278184706529No Hit
CCCATAAAGTAGAAAGCACTAC184340.2677769371919708No Hit
GGAATACCGGGTGCTGTAGGCTTT183530.2666003107455918No Hit
CAACGGAATCCCAAAAGCAGCTGT175630.25512457133029093No Hit
TATTGCACTTGTCCCGGCCTGTT155750.2262463815105211No Hit
TGTCAGTTTGTCAAATACC150690.2188960977837587No Hit
TTCTCACTACTGCACTTGACTAG149170.21668810741524513No Hit
CTCCCACTGCTTCACTTGACTAG145910.21195254912488043No Hit
TGTAAACATCCTACACTCTCAGCT144790.21032560885334414No Hit
GGAATACCGGGTGCTGTAGGCTT142960.20766730465967315No Hit
AACGGAATCCCAAAAGCAGCTG139770.2030334301362795No Hit
CAACGGAATCCCAAAAGCAGCT136440.1981961880789438No Hit
TTATAAAGCAATGAGACTGATT136160.19778945301105968No Hit
GCCTGTCTGAGCGTCGCA128120.18611034606181676No Hit
GCCTGTCTGAGCGTCGCTT127490.1851951921590776No Hit
TTCAAGTAATCCAGGATAGGC125380.18213015289752252No Hit
TGAGGTAGTAGGTTGTATAGT121130.1759564956171391No Hit
TTCACAGTGGCTAAGTTCTG121110.17592744311229025No Hit
ATCCCACCGCTGCCACCA118640.17233945876345566No Hit
TAGCAGCACGTAAATATTGGC115460.16772011049248645No Hit
TGAGGTAGTAGTTTGTGCTGTT114260.16597696020155464No Hit
CACTAGATTGTGAGCTCCTGGA109690.15933846284358943No Hit
AGCTCGGTCTGAGGCCCCTCAGT103080.1497366099910402No Hit
TGAGGTAGTAGATTGTATAGT100460.14593073185583916No Hit
TATTGCACTTGTCCCGGCCTGA98430.14298190261367955No Hit
AATGACACGATCACTCCCGTTG95450.13865307939119895No Hit
AACATTCAACGCTGTCGGTGAGTT94090.13667750906147627No Hit
TTCAAGTAATCCAGGATAGGCTT92050.13371415356689223No Hit
CCCATAAAGTAGAAAGCACTA90420.1313463744217099No Hit
TAGCTTATCAGACTGATGTTG89550.13008259046078435No Hit
CTTCTCACTACTGCACTTGACTAG89160.12951606661623152No Hit
TAGCTTATCAGACTGATGTTGACA86880.12620408106346115No Hit
TGTCAGTTTGTCAAATACCCCA86790.12607334479164126No Hit
GCCTGTCTGAGCGTCGCG86760.12602976603436794No Hit
TGTCAGTTTGTCAAATACCCCAA86280.12533250591799525No Hit
CACTAGATTGTGAGCTCCTGGAA86190.12520176964617535No Hit
TATTGCACTTGTCCCGGCCTGTAG85870.12473692956859354No Hit
CAAAGAATTCTCCTTTTGGGCT85840.12469335081132026No Hit
TCAGTGCACTACAGAACTTTGTC83750.12165736405461405No Hit
ACTGGACTTGGAGTCAGAAGG83270.12096010393824133No Hit
AGCAGCATTGTACAGGGCTATGT78500.11403108153178751No Hit
TGAGGTAGTAGGTTGTGTGGTT77490.11256393003691992No Hit
TTCACAGTGGCTAAGTTCCGC72730.10564943388289051No Hit
AGCAGCATTGTACAGGGCTATG72030.10463259621318032No Hit
AACATTCAACGCTGTCGGTGA71640.10406607236862749No Hit
TATTGCACTTGTCCCGGCCTGTTT71410.10373196856286555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)